Restriction map for
pFN21AE3261
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BbvCI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BsmI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI MamI MfeI MflI
MluI MunI NcoI NheI NotI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XhoII
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1130 TCCGGA T^CCGGA
AfaI 642 GTAC GT^AC
AlwNI 111 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 548 AGCGCT AGC^GCT
Asp700I 324 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 6 GGCGCC GGCGC^C
BbsI 277 GTCTTC GAAGAC(2/6)
BcgI 802 GCAGACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 763 GGATAC GTATCC(6/5)
BpuAI 277 GTCTTC GAAGAC(2/6)
BsaI 415 GAGACC GGTCTC(1/5)
BsaWI 1130 TCCGGA [AT]^CCGG[AT]
BseAI 1130 TCCGGA T^CCGGA
BsgI 1605 GTGCAG GTGCAG(16/14)
Bsp1407I 641 TGTACA T^GTACA
BspEI 1130 TCCGGA T^CCGGA
BsrFI 307 GCCGGC [GA]^CCGG[CT]
BsrGI 641 TGTACA T^GTACA
BstEII 1647 GGTGACC G^GT[ACGT]ACC
BstPI 1647 GGTGACC G^GT[ACGT]ACC
Cfr10I 307 GCCGGC [GA]^CCGG[CT]
EarI 1207 GAAGAG CTCTTC(1/4)
Eco47III 548 AGCGCT AGC^GCT
EcoO65I 1647 GGTGACC G^GT[ACGT]ACC
EcoRI 1244 GAATTC G^AATTC
HincII 1123 GTCAAC GT[CT]^[GA]AC
HindII 1123 GTCAAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
Ksp632I 1207 GAAGAG CTCTTC(1/4)
KspI 239 CCGCGG CCGC^GG
MroI 1130 TCCGGA T^CCGGA
MseI 1718 TTAA T^TAA
NaeI 307 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NdeI 964 CATATG CA^TATG
NgoMIV 307 GCCGGC G^CCGGC
NruI 102 TCGCGA TCG^CGA
PaeR7I 1380 CTCGAG C^TCGAG
PflFI 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1396 CAGCTG CAG^CTG
RsaI 642 GTAC GT^AC
SacII 239 CCGCGG CCGC^GG
SexAI 845 ACCTGGT A^CC[AT]GGT
SfoI 6 GGCGCC GGC^GCC
SgrAI 306 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1592 CCCGGG CCC^GGG
SspBI 641 TGTACA T^GTACA
TfiI 1104 GAATC G^A[AT]TC
Tru9I 1718 TTAA T^TAA
Tth111I 679 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1380 CTCGAG C^TCGAG
XmaCI 1592 CCCGGG C^CCGGG
XmaI 1592 CCCGGG C^CCGGG
XmnI 324 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 58 GAGCTC G[AT]GC[AT]^C
754 GAGCTC
BalI 888 TGGCCA TGG^CCA
1637 TGGCCA
BanI 6 GGCGCC G^G[CT][GA]CC
1363 GGTGCC
BlpI 1029 GCTTAGC GC^T[ACGT]AGC
1570 GCTGAGC
Bpu1102I 1029 GCTTAGC GC^T[ACGT]AGC
1570 GCTGAGC
BsiHKAI 58 GAGCTC G[AT]GC[AT]^C
754 GAGCTC
BsrBI 1803 CCGCTC CCGCTC(-3/-3)
257 GAGCGG
BstXI 687 CCAGAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCATCACCCTGG
CelII 1029 GCTTAGC GC^T[ACGT]AGC
1570 GCTGAGC
EcoNI 555 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1353 CCTGGTTCAGG
EcoT14I 143 CCAAGG C^C[AT][AT]GG
768 CCTTGG
HindIII 1684 AAGCTT A^AGCTT
1744 AAGCTT
MluNI 888 TGGCCA TGG^CCA
1637 TGGCCA
MscI 888 TGGCCA TGG^CCA
1637 TGGCCA
NspI 510 GCATGT [GA]CATG^[CT]
1289 ACATGC
PflMI 380 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1560 CCAGAGAGTGG
PpuMI 261 GGGACCT [GA]G^G[AT]CC[CT]
881 AGGTCCC
SacI 58 GAGCTC GAGCT^C
754 GAGCTC
SmlI 1380 CTCGAG C^T[CT][GA]AG
1747 CTTGAG
StyI 143 CCAAGG C^C[AT][AT]GG
768 CCTTGG
TaqI 56 TCGA T^CGA
1381 TCGA
TthHB8I 56 TCGA T^CGA
1381 TCGA
Van91I 380 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1560 CCAGAGAGTGG
XbaI 816 TCTAGA T^CTAGA
1107 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AocI ApaI ApoI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I Tsp509I
TspRI XcmI