################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MluI MroI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI TfiI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 327 GAATTT [GA]^AATT[CT] AcyI 636 GGCGTC G[GA]^CG[CT]C AflIII 173 ACACGT A^C[GA][CT]GT AhdI 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 327 GAATTT [GA]^AATT[CT] AspEI 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 708 CTCGGG C^[CT]CG[GA]G BanII 1240 GAGCTC G[GA]GC[CT]^C BciVI 261 GGATAC GTATCC(6/5) BcnI 1344 CCGGG CC^[GC]GG BglII 197 AGATCT A^GATCT BsaBI 660 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 636 GGCGTC G[GA]^CG[CT]C BsaJI 249 CCTCGG C^C[ACGT][ACGT]GG BseRI 524 GAGGAG GAGGAG(10/8) BsoBI 708 CTCGGG C^[CT]CG[GA]G Bsp1407I 1399 TGTACA T^GTACA BspHI 1388 TCATGA T^CATGA BsrDI 125 GCAATG GCAATG(2/0) BsrFI 1071 GCCGGT [GA]^CCGG[CT] BsrGI 1399 TGTACA T^GTACA BssSI 1447 CTCGTG CACGAG(-5/-1) BstYI 197 AGATCT [GA]^GATC[CT] Cfr10I 1071 GCCGGT [GA]^CCGG[CT] DraII 1105 GGGTCCC [GA]G^G[ACGT]CC[CT] Eam1105I 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1105 GGGTCCC [GA]G^G[ACGT]CC[CT] HaeII 1068 AGCGCC [GA]GCGC^[CT] HgaI 637 GCGTC GACGC(5/10) Hin1I 636 GGCGTC G[GA]^CG[CT]C MamI 660 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1403 CAATTG C^AATTG MflI 197 AGATCT [GA]^GATC[CT] MunI 1403 CAATTG C^AATTG NciI 1344 CCGGG CC^[GC]GG PacI 757 TTAATTAA TTAAT^TAA PflFI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 467 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1105 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 1225 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1388 TCATGA T^CATGA SacI 1240 GAGCTC GAGCT^C SapI 359 GCTCTTC GCTCTTC(1/4) SfaNI 308 GCATC GCATC(5/9) SspBI 1399 TGTACA T^GTACA SspI 1437 AATATT AAT^ATT Tth111I 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 467 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 197 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 976 GCGG CCGC(-3/-1) 1222 GCGG AspHI 357 GTGCTC G[AT]GC[AT]^C 1240 GAGCTC BalI 7 TGGCCA TGG^CCA 1268 TGGCCA BbsI 739 GAAGAC GAAGAC(2/6) 778 GAAGAC BfaI 586 CTAG C^TAG 1354 CTAG BmyI 357 GTGCTC G[AGT]GC[ACT]^C 1240 GAGCTC BpuAI 739 GAAGAC GAAGAC(2/6) 778 GAAGAC BsgI 227 GTGCAG GTGCAG(16/14) 643 GTGCAG BsiHKAI 357 GTGCTC G[AT]GC[AT]^C 1240 GAGCTC Bsp1286I 357 GTGCTC G[AGT]GC[ACT]^C 1240 GAGCTC CfoI 391 GCGC GCG^C 1069 GCGC EaeI 7 TGGCCA [CT]^GGCC[GA] 1268 TGGCCA HhaI 391 GCGC GCG^C 1069 GCGC HinP1I 391 GCGC G^CGC 1069 GCGC HindIII 843 AAGCTT A^AGCTT 1262 AAGCTT MaeI 586 CTAG C^TAG 1354 CTAG MaeII 175 ACGT A^CGT 1007 ACGT MluNI 7 TGGCCA TGG^CCA 1268 TGGCCA MscI 7 TGGCCA TGG^CCA 1268 TGGCCA MslI 52 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1383 CATTCTCATG NspI 353 GCATGT [GA]CATG^[CT] 856 GCATGT PstI 460 CTGCAG CTGCA^G 1313 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AfaI AluI AlwI AseI AsnI AvaII BbvI BpmI BsaI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PshBI RsaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I TspRI