Restriction map for
pFN21AE6462
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BspMI BsrBI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MluI MroI MspA1I MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TaqI TfiI TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 327 GAATTT [GA]^AATT[CT]
AcyI 636 GGCGTC G[GA]^CG[CT]C
AflIII 173 ACACGT A^C[GA][CT]GT
AhdI 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 327 GAATTT [GA]^AATT[CT]
AspEI 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 708 CTCGGG C^[CT]CG[GA]G
BanII 1240 GAGCTC G[GA]GC[CT]^C
BciVI 261 GGATAC GTATCC(6/5)
BcnI 1344 CCGGG CC^[GC]GG
BglII 197 AGATCT A^GATCT
BsaBI 660 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 636 GGCGTC G[GA]^CG[CT]C
BsaJI 249 CCTCGG C^C[ACGT][ACGT]GG
BseRI 524 GAGGAG GAGGAG(10/8)
BsoBI 708 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1399 TGTACA T^GTACA
BspHI 1388 TCATGA T^CATGA
BsrDI 125 GCAATG GCAATG(2/0)
BsrFI 1071 GCCGGT [GA]^CCGG[CT]
BsrGI 1399 TGTACA T^GTACA
BssSI 1447 CTCGTG CACGAG(-5/-1)
BstYI 197 AGATCT [GA]^GATC[CT]
Cfr10I 1071 GCCGGT [GA]^CCGG[CT]
DraII 1105 GGGTCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 985 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1105 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 1068 AGCGCC [GA]GCGC^[CT]
HgaI 637 GCGTC GACGC(5/10)
Hin1I 636 GGCGTC G[GA]^CG[CT]C
MamI 660 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1403 CAATTG C^AATTG
MflI 197 AGATCT [GA]^GATC[CT]
MunI 1403 CAATTG C^AATTG
NciI 1344 CCGGG CC^[GC]GG
PacI 757 TTAATTAA TTAAT^TAA
PflFI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 467 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1105 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1225 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1388 TCATGA T^CATGA
SacI 1240 GAGCTC GAGCT^C
SapI 359 GCTCTTC GCTCTTC(1/4)
SfaNI 308 GCATC GCATC(5/9)
SspBI 1399 TGTACA T^GTACA
SspI 1437 AATATT AAT^ATT
Tth111I 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 467 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 197 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 976 GCGG CCGC(-3/-1)
1222 GCGG
AspHI 357 GTGCTC G[AT]GC[AT]^C
1240 GAGCTC
BalI 7 TGGCCA TGG^CCA
1268 TGGCCA
BbsI 739 GAAGAC GAAGAC(2/6)
778 GAAGAC
BfaI 586 CTAG C^TAG
1354 CTAG
BmyI 357 GTGCTC G[AGT]GC[ACT]^C
1240 GAGCTC
BpuAI 739 GAAGAC GAAGAC(2/6)
778 GAAGAC
BsgI 227 GTGCAG GTGCAG(16/14)
643 GTGCAG
BsiHKAI 357 GTGCTC G[AT]GC[AT]^C
1240 GAGCTC
Bsp1286I 357 GTGCTC G[AGT]GC[ACT]^C
1240 GAGCTC
CfoI 391 GCGC GCG^C
1069 GCGC
EaeI 7 TGGCCA [CT]^GGCC[GA]
1268 TGGCCA
HhaI 391 GCGC GCG^C
1069 GCGC
HinP1I 391 GCGC G^CGC
1069 GCGC
HindIII 843 AAGCTT A^AGCTT
1262 AAGCTT
MaeI 586 CTAG C^TAG
1354 CTAG
MaeII 175 ACGT A^CGT
1007 ACGT
MluNI 7 TGGCCA TGG^CCA
1268 TGGCCA
MscI 7 TGGCCA TGG^CCA
1268 TGGCCA
MslI 52 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1383 CATTCTCATG
NspI 353 GCATGT [GA]CATG^[CT]
856 GCATGT
PstI 460 CTGCAG CTGCA^G
1313 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AfaI AluI AlwI AseI AsnI
AvaII BbvI BpmI BsaI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI