Restriction map for
pFN21AE6643
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII Hin1I HincII HindII
HpaI HpaII KasI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 943 CGCG CG^CG
AocI 97 CCTAAGG CC^T[ACGT]AGG
Asp700I 844 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 529 GAGCTC G[AT]GC[AT]^C
AspI 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 529 GAGCTC G[GA]GC[CT]^C
BglII 854 AGATCT A^GATCT
BmyI 529 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 529 GAGCTC G[AT]GC[AT]^C
BsmI 50 GCATTC GAATGC(1/-1)
Bsp1286I 529 GAGCTC G[AGT]GC[ACT]^C
BspHI 966 TCATGA T^CATGA
BstUI 943 CGCG CG^CG
Bsu36I 97 CCTAAGG CC^T[ACGT]AGG
CfoI 942 GCGC GCG^C
DsaI 365 CCATGG C^C[GA][CT]GG
Eco81I 97 CCTAAGG CC^T[ACGT]AGG
EcoRV 241 GATATC GAT^ATC
EcoT14I 365 CCATGG C^C[AT][AT]GG
HgaI 475 GACGC GACGC(5/10)
HhaI 942 GCGC GCG^C
HinP1I 942 GCGC G^CGC
MaeIII 20 GTCAC ^GT[ACGT]AC
MslI 151 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 523 CAGCTG C[AC]G^C[GT]G
MvnI 943 CGCG CG^CG
NcoI 365 CCATGG C^CATGG
PflFI 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 551 GACTC GAGTC(4/5)
PpuMI 320 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 523 CAGCTG CAG^CTG
RcaI 966 TCATGA T^CATGA
SacI 529 GAGCTC GAGCT^C
SexAI 575 ACCAGGT A^CC[AT]GGT
SfaNI 721 GATGC GCATC(5/9)
StyI 365 CCATGG C^C[AT][AT]GG
TaqI 91 TCGA T^CGA
TfiI 678 GAATC G^A[AT]TC
Tsp45I 20 GTCAC ^GT[GC]AC
Tth111I 637 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 91 TCGA T^CGA
XmnI 844 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Acc65I 70 GGTACC G^GTACC
143 GGTACC
AcsI 619 AAATTT [GA]^AATT[CT]
846 AAATTT
ApoI 619 AAATTT [GA]^AATT[CT]
846 AAATTT
Asp718I 70 GGTACC G^GTACC
143 GGTACC
BanI 70 GGTACC G^G[CT][GA]CC
143 GGTACC
BfaI 331 CTAG C^TAG
398 CTAG
BsmFI 667 GGGAC GGGAC(10/14)
280 GTCCC
BsrI 565 CCAGT ACTGG(1/-1)
671 CCAGT
DraII 160 GGGGCCT [GA]G^G[ACGT]CC[CT]
320 GGGTCCT
EcoO109I 160 GGGGCCT [GA]G^G[ACGT]CC[CT]
320 GGGTCCT
HindIII 464 AAGCTT A^AGCTT
819 AAGCTT
HinfI 551 GACTC G^A[ACGT]TC
678 GAATC
KpnI 70 GGTACC GGTAC^C
143 GGTACC
MaeI 331 CTAG C^TAG
398 CTAG
TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^
873 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BpmI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HphI ItaI MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I XhoII