Restriction map for
pFN21AE5537
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MroI MseI
MspA1I MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1144 GTCGAC GT^[AC][GT]AC
AccII 26 CGCG CG^CG
ApaI 880 GGGCCC GGGCC^C
Asp700I 839 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 554 GGCACC G^G[CT][GA]CC
BsaBI 957 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 427 GAGGAG GAGGAG(10/8)
BsiEI 999 CGGCCG CG[GA][CT]^CG
BsmBI 1255 GAGACG CGTCTC(1/5)
BspHI 314 TCATGA T^CATGA
BstAPI 854 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 26 CGCG CG^CG
BstXI 559 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 999 CGGCCG C^GGCCG
EclXI 999 CGGCCG C^GGCCG
Eco52I 999 CGGCCG C^GGCCG
EcoRI 581 GAATTC G^AATTC
HaeII 1266 GGCGCT [GA]GCGC^[CT]
HgaI 24 GACGC GACGC(5/10)
HincII 1144 GTCGAC GT[CT]^[GA]AC
HindII 1144 GTCGAC GT[CT]^[GA]AC
HindIII 278 AAGCTT A^AGCTT
MaeII 1258 ACGT A^CGT
MamI 957 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 26 CGCG CG^CG
PflFI 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 314 TCATGA T^CATGA
SalI 1144 GTCGAC G^TCGAC
SexAI 119 ACCAGGT A^CC[AT]GGT
SmaI 590 CCCGGG CCC^GGG
StuI 1064 AGGCCT AGG^CCT
Tsp45I 709 GTCAC ^GT[GC]AC
Tth111I 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
XmnI 839 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 331 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
813 CAGGAACTG
AvaI 166 CTCGGG C^[CT]CG[GA]G
590 CCCGGG
BalI 137 TGGCCA TGG^CCA
1127 TGGCCA
BbsI 350 GAAGAC GAAGAC(2/6)
67 GTCTTC
BpuAI 350 GAAGAC GAAGAC(2/6)
67 GTCTTC
BsmI 681 GAATGC GAATGC(1/-1)
465 GCATTC
BsoBI 166 CTCGGG C^[CT]CG[GA]G
590 CCCGGG
BstYI 171 GGATCT [GA]^GATC[CT]
804 GGATCT
Cac8I 374 GCCGGC GC[ACGT]^[ACGT]GC
1224 GCCGGC
DraII 179 AGGGCCT [GA]G^G[ACGT]CC[CT]
880 GGGCCCT
EcoO109I 179 AGGGCCT [GA]G^G[ACGT]CC[CT]
880 GGGCCCT
FauI 371 CCCGC CCCGC(4/6)
20 GCGGG
MaeIII 404 GTTAC ^GT[ACGT]AC
709 GTCAC
MflI 171 GGATCT [GA]^GATC[CT]
804 GGATCT
MluNI 137 TGGCCA TGG^CCA
1127 TGGCCA
MscI 137 TGGCCA TGG^CCA
1127 TGGCCA
MslI 327 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
795 CATCGGCGTG
NaeI 374 GCCGGC GCC^GGC
1224 GCCGGC
NgoMIV 374 GCCGGC G^CCGGC
1224 GCCGGC
PflMI 192 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
417 CCACACCCTGG
PleI 131 GAGTC GAGTC(4/5)
289 GAGTC
SacI 703 GAGCTC GAGCT^C
1189 GAGCTC
TfiI 785 GAATC G^A[AT]TC
1296 GAATC
Van91I 192 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
417 CCACACCCTGG
XhoII 171 GGATCT [GA]^GATC[CT]
804 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XcmI