################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspHI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BstAPI BstBI BstEII BstPI Bsu36I CelII CfoI ClaI CpoI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MroI MslI NarI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI SspBI SspI StuI TfiI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 687 GTATAC GT^[AC][GT]AC AccII 722 CGCG CG^CG ApaI 23 GGGCCC GGGCC^C AseI 85 ATTAAT AT^TAAT AsnI 85 ATTAAT AT^TAAT AspI 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 545 CCCGAG C^[CT]CG[GA]G BamHI 612 GGATCC G^GATCC BanII 23 GGGCCC G[GA]GC[CT]^C BcnI 1157 CCGGG CC^[GC]GG BsoBI 545 CCCGAG C^[CT]CG[GA]G BsrDI 405 CATTGC GCAATG(2/0) BssSI 391 CACGAG CACGAG(-5/-1) Bst1107I 687 GTATAC GTA^TAC BstUI 722 CGCG CG^CG BstZ17I 687 GTATAC GTA^TAC DraI 226 TTTAAA TTT^AAA DsaI 831 CCATGG C^C[GA][CT]GG EcoRI 132 GAATTC G^AATTC EcoT14I 831 CCATGG C^C[AT][AT]GG FseI 512 GGCCGGCC GGCCGG^CC HgaI 720 GACGC GACGC(5/10) HincII 122 GTCAAC GT[CT]^[GA]AC HindII 122 GTCAAC GT[CT]^[GA]AC HinfI 120 GAGTC G^A[ACGT]TC MaeII 1213 ACGT A^CGT MaeIII 265 GTGAC ^GT[ACGT]AC MfeI 269 CAATTG C^AATTG MspA1I 934 CAGCTG C[AC]G^C[GT]G MunI 269 CAATTG C^AATTG MvnI 722 CGCG CG^CG NaeI 513 GCCGGC GCC^GGC NciI 1157 CCGGG CC^[GC]GG NcoI 831 CCATGG C^CATGG NdeI 950 CATATG CA^TATG NgoMIV 513 GCCGGC G^CCGGC NheI 1084 GCTAGC G^CTAGC NspI 1242 GCATGT [GA]CATG^[CT] PflFI 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 120 GAGTC GAGTC(4/5) PshBI 85 ATTAAT AT^TAAT PvuII 934 CAGCTG CAG^CTG SbfI 279 CCTGCAGG CCTGCA^GG SpeI 126 ACTAGT A^CTAGT Sse8387I 279 CCTGCAGG CCTGCA^GG StyI 831 CCATGG C^C[AT][AT]GG SwaI 225 ATTTAAAT ATTT^AAAT Tsp45I 265 GTGAC ^GT[GC]AC Tth111I 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 39 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 934 CAGCTGCTG BalI 48 TGGCCA TGG^CCA 665 TGGCCA BbsI 717 GAAGAC GAAGAC(2/6) 1227 GAAGAC BciVI 8 GGATAC GTATCC(6/5) 945 GTATCC BmyI 23 GGGCCC G[AGT]GC[ACT]^C 1038 GGGCAC BpuAI 717 GAAGAC GAAGAC(2/6) 1227 GAAGAC BsgI 919 CTGCAC GTGCAG(16/14) 1065 CTGCAC BsmAI 101 GAGAC GTCTC(1/5) 1210 GAGAC BsmBI 101 GAGACG CGTCTC(1/5) 1210 GAGACG BsmI 528 GAATGC GAATGC(1/-1) 1132 GCATTC Bsp1286I 23 GGGCCC G[AGT]GC[ACT]^C 1038 GGGCAC BsrFI 464 ACCGGC [GA]^CCGG[CT] 513 GCCGGC BstXI 885 CCAAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1016 CCAAAGCTCTGG Cfr10I 464 ACCGGC [GA]^CCGG[CT] 513 GCCGGC FauI 1088 GCGGG CCCGC(4/6) 1248 GCGGG MluNI 48 TGGCCA TGG^CCA 665 TGGCCA MscI 48 TGGCCA TGG^CCA 665 TGGCCA MseI 86 TTAA T^TAA 227 TTAA PflMI 369 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACGAGATGG Tru9I 86 TTAA T^TAA 227 TTAA Van91I 369 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACGAGATGG XcmI 763 CCAAATAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 845 CCAATTCGGAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BsaJI BseRI BsiEI BsiYI BslI BsmFI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeI MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I XhoII