Restriction map for
pFN21AE1892
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspHI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstPI Bsu36I CelII
CfoI ClaI CpoI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MluI MroI MslI NarI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI SspBI
SspI StuI TfiI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 687 GTATAC GT^[AC][GT]AC
AccII 722 CGCG CG^CG
ApaI 23 GGGCCC GGGCC^C
AseI 85 ATTAAT AT^TAAT
AsnI 85 ATTAAT AT^TAAT
AspI 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 545 CCCGAG C^[CT]CG[GA]G
BamHI 612 GGATCC G^GATCC
BanII 23 GGGCCC G[GA]GC[CT]^C
BcnI 1157 CCGGG CC^[GC]GG
BsoBI 545 CCCGAG C^[CT]CG[GA]G
BsrDI 405 CATTGC GCAATG(2/0)
BssSI 391 CACGAG CACGAG(-5/-1)
Bst1107I 687 GTATAC GTA^TAC
BstUI 722 CGCG CG^CG
BstZ17I 687 GTATAC GTA^TAC
DraI 226 TTTAAA TTT^AAA
DsaI 831 CCATGG C^C[GA][CT]GG
EcoRI 132 GAATTC G^AATTC
EcoT14I 831 CCATGG C^C[AT][AT]GG
FseI 512 GGCCGGCC GGCCGG^CC
HgaI 720 GACGC GACGC(5/10)
HincII 122 GTCAAC GT[CT]^[GA]AC
HindII 122 GTCAAC GT[CT]^[GA]AC
HinfI 120 GAGTC G^A[ACGT]TC
MaeII 1213 ACGT A^CGT
MaeIII 265 GTGAC ^GT[ACGT]AC
MfeI 269 CAATTG C^AATTG
MspA1I 934 CAGCTG C[AC]G^C[GT]G
MunI 269 CAATTG C^AATTG
MvnI 722 CGCG CG^CG
NaeI 513 GCCGGC GCC^GGC
NciI 1157 CCGGG CC^[GC]GG
NcoI 831 CCATGG C^CATGG
NdeI 950 CATATG CA^TATG
NgoMIV 513 GCCGGC G^CCGGC
NheI 1084 GCTAGC G^CTAGC
NspI 1242 GCATGT [GA]CATG^[CT]
PflFI 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 120 GAGTC GAGTC(4/5)
PshBI 85 ATTAAT AT^TAAT
PvuII 934 CAGCTG CAG^CTG
SbfI 279 CCTGCAGG CCTGCA^GG
SpeI 126 ACTAGT A^CTAGT
Sse8387I 279 CCTGCAGG CCTGCA^GG
StyI 831 CCATGG C^C[AT][AT]GG
SwaI 225 ATTTAAAT ATTT^AAAT
Tsp45I 265 GTGAC ^GT[GC]AC
Tth111I 720 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 39 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
934 CAGCTGCTG
BalI 48 TGGCCA TGG^CCA
665 TGGCCA
BbsI 717 GAAGAC GAAGAC(2/6)
1227 GAAGAC
BciVI 8 GGATAC GTATCC(6/5)
945 GTATCC
BmyI 23 GGGCCC G[AGT]GC[ACT]^C
1038 GGGCAC
BpuAI 717 GAAGAC GAAGAC(2/6)
1227 GAAGAC
BsgI 919 CTGCAC GTGCAG(16/14)
1065 CTGCAC
BsmAI 101 GAGAC GTCTC(1/5)
1210 GAGAC
BsmBI 101 GAGACG CGTCTC(1/5)
1210 GAGACG
BsmI 528 GAATGC GAATGC(1/-1)
1132 GCATTC
Bsp1286I 23 GGGCCC G[AGT]GC[ACT]^C
1038 GGGCAC
BsrFI 464 ACCGGC [GA]^CCGG[CT]
513 GCCGGC
BstXI 885 CCAAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1016 CCAAAGCTCTGG
Cfr10I 464 ACCGGC [GA]^CCGG[CT]
513 GCCGGC
FauI 1088 GCGGG CCCGC(4/6)
1248 GCGGG
MluNI 48 TGGCCA TGG^CCA
665 TGGCCA
MscI 48 TGGCCA TGG^CCA
665 TGGCCA
MseI 86 TTAA T^TAA
227 TTAA
PflMI 369 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACGAGATGG
Tru9I 86 TTAA T^TAA
227 TTAA
Van91I 369 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACGAGATGG
XcmI 763 CCAAATAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
845 CCAATTCGGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EagI EclXI Eco52I
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeI
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp509I TspRI TthHB8I XhoII