Restriction map for
pFN21AE6179
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
TseI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 204 GTAC GT^AC
AlwI 270 GATCC GGATC(4/5)
AspHI 283 GAGCAC G[AT]GC[AT]^C
AviII 317 TGCGCA TGC^GCA
BciVI 123 GGATAC GTATCC(6/5)
BcnI 171 CCGGG CC^[GC]GG
BmyI 283 GAGCAC G[AGT]GC[ACT]^C
BpmI 265 CTGGAG CTGGAG(16/14)
BsiHKAI 283 GAGCAC G[AT]GC[AT]^C
BsmAI 35 GAGAC GTCTC(1/5)
BsmBI 35 GAGACG CGTCTC(1/5)
Bsp1286I 283 GAGCAC G[AGT]GC[ACT]^C
BspMI 350 ACCTGC ACCTGC(4/8)
BstXI 101 CCAATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 269 AGATCC [GA]^GATC[CT]
Cac8I 225 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 323 GGGCC G^G[ACGT]CC
FspI 317 TGCGCA TGC^GCA
HaeIII 324 GGCC GG^CC
HinfI 307 GACTC G^A[ACGT]TC
HphI 48 GGTGA GGTGA(8/7)
MaeIII 49 GTGAC ^GT[ACGT]AC
MflI 269 AGATCC [GA]^GATC[CT]
MseI 83 TTAA T^TAA
MspA1I 257 CCGCTG C[AC]G^C[GT]G
MwoI 229 GCCCTGGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 171 CCGGG CC^[GC]GG
NdeI 290 CATATG CA^TATG
PleI 307 GACTC GAGTC(4/5)
RsaI 204 GTAC GT^AC
Sau96I 323 GGGCC G^G[ACGT]CC
SfaNI 185 GCATC GCATC(5/9)
SphI 225 GCATGC GCATG^C
TaqI 143 TCGA T^CGA
Tru9I 83 TTAA T^TAA
Tsp45I 49 GTGAC ^GT[GC]AC
TthHB8I 143 TCGA T^CGA
XcmI 101 CCAATTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 269 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 235 GACGCC G[GA]^CG[CT]C
295 GACGCC
AluI 17 AGCT AG^CT
80 AGCT
BbsI 145 GAAGAC GAAGAC(2/6)
346 GAAGAC
BpuAI 145 GAAGAC GAAGAC(2/6)
346 GAAGAC
BsaHI 235 GACGCC G[GA]^CG[CT]C
295 GACGCC
BsrI 52 ACTGG ACTGG(1/-1)
201 CCAGT
CfoI 318 GCGC GCG^C
354 GCGC
EcoT14I 278 CCAAGG C^C[AT][AT]GG
302 CCAAGG
FauI 256 CCCGC CCCGC(4/6)
4 GCGGG
HapII 156 CCGG C^CGG
171 CCGG
HgaI 235 GACGC GACGC(5/10)
295 GACGC
HhaI 318 GCGC GCG^C
354 GCGC
Hin1I 235 GACGCC G[GA]^CG[CT]C
295 GACGCC
HinP1I 318 GCGC G^CGC
354 GCGC
HpaII 156 CCGG C^CGG
171 CCGG
MboII 145 GAAGA GAAGA(8/7)
346 GAAGA
MslI 21 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
286 CACCCATATG
MspI 156 CCGG C^CGG
171 CCGG
NlaIII 226 CATG CATG^
333 CATG
NspI 225 GCATGC [GA]CATG^[CT]
332 GCATGT
StyI 278 CCAAGG C^C[AT][AT]GG
302 CCAAGG
Tsp509I 103 AATT ^AATT
161 AATT
TspRI 26 CAGTG CA[GC]TG[ACGT][ACGT]^
339 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
BstF5I BstNI DpnII EcoRII Fnu4HI
FokI ItaI MboI MnlI MvaI
NdeII Sau3AI ScrFI