Restriction map for
pFN21AE2197
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraII
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI MaeII MamI MluI MluNI
MroI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Alw44I 23 GTGCAC G^TGCAC
AlwNI 1027 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 23 GTGCAC G^TGCAC
AspHI 23 GTGCAC G[AT]GC[AT]^C
BamHI 529 GGATCC G^GATCC
BanI 309 GGCACC G^G[CT][GA]CC
BbsI 91 GTCTTC GAAGAC(2/6)
BbvCI 533 CCTCAGC CCTCAGC(-5/-2)
BbvI 51 GCAGC GCAGC(8/12)
BciVI 173 GTATCC GTATCC(6/5)
BcnI 1083 CCGGG CC^[GC]GG
BfaI 314 CTAG C^TAG
BglI 139 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 553 CTGGAG CTGGAG(16/14)
BpuAI 91 GTCTTC GAAGAC(2/6)
BseRI 472 GAGGAG GAGGAG(10/8)
BsiEI 879 CGGCCG CG[GA][CT]^CG
BsiHKAI 23 GTGCAC G[AT]GC[AT]^C
BsmFI 110 GGGAC GGGAC(10/14)
BssHII 85 GCGCGC G^CGCGC
BstAPI 1100 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 432 TTCGAA TT^CGAA
BstXI 252 CCACGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 529 GGATCC [GA]^GATC[CT]
DraI 907 TTTAAA TTT^AAA
DsaI 140 CCGCGG C^C[GA][CT]GG
EagI 879 CGGCCG C^GGCCG
EclXI 879 CGGCCG C^GGCCG
Eco52I 879 CGGCCG C^GGCCG
EcoRI 295 GAATTC G^AATTC
EcoT14I 749 CCAAGG C^C[AT][AT]GG
FauI 180 GCGGG CCCGC(4/6)
HgaI 89 GCGTC GACGC(5/10)
KspI 140 CCGCGG CCGC^GG
MaeI 314 CTAG C^TAG
MfeI 794 CAATTG C^AATTG
MflI 529 GGATCC [GA]^GATC[CT]
MunI 794 CAATTG C^AATTG
NaeI 4 GCCGGC GCC^GGC
NciI 1083 CCGGG CC^[GC]GG
NgoMIV 4 GCCGGC G^CCGGC
NlaIII 891 CATG CATG^
NspV 432 TTCGAA TT^CGAA
PleI 617 GACTC GAGTC(4/5)
PstI 1048 CTGCAG CTGCA^G
SacII 140 CCGCGG CCGC^GG
SfaNI 810 GATGC GCATC(5/9)
SfuI 432 TTCGAA TT^CGAA
StyI 749 CCAAGG C^C[AT][AT]GG
TaqI 433 TCGA T^CGA
TfiI 1092 GATTC G^A[AT]TC
TseI 51 GCAGC G^C[AT]GC
Tsp45I 497 GTGAC ^GT[GC]AC
TthHB8I 433 TCGA T^CGA
XcmI 1004 CCACTGCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 529 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 862 GTAC GT^AC
1080 GTAC
Asp700I 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAAAAGTTC
BlpI 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
BmyI 23 GTGCAC G[AGT]GC[ACT]^C
745 GTGCCC
Bpu1102I 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
Bsp1286I 23 GTGCAC G[AGT]GC[ACT]^C
745 GTGCCC
BspMI 27 ACCTGC ACCTGC(4/8)
1050 GCAGGT
BsrFI 4 GCCGGC [GA]^CCGG[CT]
65 GCCGGT
BstF5I 390 GGATG GGATG(2/0)
857 GGATG
CelII 831 GCTGAGC GC^T[ACGT]AGC
895 GCTCAGC
CfoI 85 GCGC GCG^C
87 GCGC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
65 GCCGGT
EaeI 2 TGGCCG [CT]^GGCC[GA]
879 CGGCCG
FokI 390 GGATG GGATG(9/13)
857 GGATG
HaeIII 3 GGCC GG^CC
880 GGCC
HhaI 85 GCGC GCG^C
87 GCGC
HinP1I 85 GCGC G^CGC
87 GCGC
HinfI 617 GACTC G^A[ACGT]TC
1092 GATTC
MseI 403 TTAA T^TAA
908 TTAA
MslI 97 CACCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
253 CACGAAAATG
MspA1I 140 CCGCGG C[AC]G^C[GT]G
178 CAGCGG
RsaI 862 GTAC GT^AC
1080 GTAC
SapI 130 GAAGAGC GCTCTTC(1/4)
435 GAAGAGC
SexAI 396 ACCTGGT A^CC[AT]GGT
1031 ACCTGGT
SfcI 653 CTATAG C^T[GA][CT]AG
1048 CTGCAG
Tru9I 403 TTAA T^TAA
908 TTAA
XmnI 234 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1018 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BsaJI BsiYI BslI
BsrI BssKI BstNI BstUI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HapII HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI Tsp509I
TspRI