Restriction map for
pFN21AE5757
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 460 CGCG CG^CG
AcsI 797 AAATTC [GA]^AATT[CT]
ApaI 179 GGGCCC GGGCC^C
ApoI 797 AAATTC [GA]^AATT[CT]
AseI 345 ATTAAT AT^TAAT
AsnI 345 ATTAAT AT^TAAT
Asp700I 166 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1328 CCCGAG C^[CT]CG[GA]G
AviII 1550 TGCGCA TGC^GCA
AvrII 436 CCTAGG C^CTAGG
BalI 1388 TGGCCA TGG^CCA
BamHI 1772 GGATCC G^GATCC
BbsI 390 GAAGAC GAAGAC(2/6)
BclI 843 TGATCA T^GATCA
BglI 319 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 436 CCTAGG C^CTAGG
BpuAI 390 GAAGAC GAAGAC(2/6)
BsgI 1471 CTGCAC GTGCAG(16/14)
BsmI 1119 GCATTC GAATGC(1/-1)
BsoBI 1328 CCCGAG C^[CT]CG[GA]G
BspHI 1148 TCATGA T^CATGA
BssSI 1648 CACGAG CACGAG(-5/-1)
BstAPI 2242 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 460 CGCG CG^CG
BstXI 1065 CCAACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 566 CCGTGG C^C[GA][CT]GG
EcoNI 2230 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 843 TGATCA T^GATCA
FseI 3 GGCCGGCC GGCCGG^CC
FspI 1550 TGCGCA TGC^GCA
HaeII 2013 GGCGCT [GA]GCGC^[CT]
HincII 1240 GTTGAC GT[CT]^[GA]AC
HindII 1240 GTTGAC GT[CT]^[GA]AC
HindIII 1273 AAGCTT A^AGCTT
MaeII 221 ACGT A^CGT
MluNI 1388 TGGCCA TGG^CCA
MscI 1388 TGGCCA TGG^CCA
MvnI 460 CGCG CG^CG
PshBI 345 ATTAAT AT^TAAT
PstI 1361 CTGCAG CTGCA^G
RcaI 1148 TCATGA T^CATGA
SpeI 1531 ACTAGT A^CTAGT
SphI 1653 GCATGC GCATG^C
XcmI 1955 CCAAGGCTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 166 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AspHI 1645 GAGCAC G[AT]GC[AT]^C
2240 GAGCAC
BanI 1306 GGCACC G^G[CT][GA]CC
1899 GGTGCC
BanII 179 GGGCCC G[GA]GC[CT]^C
1926 GGGCTC
BcnI 1310 CCCGG CC^[GC]GG
1943 CCGGG
BpmI 1190 CTCCAG CTGGAG(16/14)
58 CTGGAG
BsiHKAI 1645 GAGCAC G[AT]GC[AT]^C
2240 GAGCAC
BstEII 780 GGTGACC G^GT[ACGT]ACC
1251 GGTGACC
BstPI 780 GGTGACC G^GT[ACGT]ACC
1251 GGTGACC
BstYI 1772 GGATCC [GA]^GATC[CT]
1798 AGATCC
EcoO65I 780 GGTGACC G^GT[ACGT]ACC
1251 GGTGACC
EcoT14I 436 CCTAGG C^C[AT][AT]GG
1955 CCAAGG
MflI 1772 GGATCC [GA]^GATC[CT]
1798 AGATCC
MslI 519 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1648 CACGAGCATG
MspA1I 55 CAGCTG C[AC]G^C[GT]G
2194 CAGCTG
NaeI 4 GCCGGC GCC^GGC
1226 GCCGGC
NciI 1310 CCCGG CC^[GC]GG
1943 CCGGG
NgoMIV 4 GCCGGC G^CCGGC
1226 GCCGGC
NspI 127 GCATGT [GA]CATG^[CT]
1653 GCATGC
PleI 1033 GACTC GAGTC(4/5)
1152 GACTC
PpuMI 2006 AGGTCCT [GA]G^G[AT]CC[CT]
2226 GGGACCT
PvuII 55 CAGCTG CAG^CTG
2194 CAGCTG
StyI 436 CCTAGG C^C[AT][AT]GG
1955 CCAAGG
XhoII 1772 GGATCC [GA]^GATC[CT]
1798 AGATCC
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BfaI BmyI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I