Restriction map for
pFN21AE1175
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflIII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1810 GACGTC GACGT^C
AciI 222 CCGC CCGC(-3/-1)
AcyI 1810 GACGTC G[GA]^CG[CT]C
AflII 169 CTTAAG C^TTAAG
AgeI 543 ACCGGT A^CCGGT
AlwI 82 GATCC GGATC(4/5)
AlwNI 1238 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 631 CCTCAGG CC^T[ACGT]AGG
BalI 1401 TGGCCA TGG^CCA
BanII 574 GAGCCC G[GA]GC[CT]^C
BbsI 1041 GTCTTC GAAGAC(2/6)
BfrI 169 CTTAAG C^TTAAG
BglII 1975 AGATCT A^GATCT
BmyI 574 GAGCCC G[AGT]GC[ACT]^C
BpuAI 1041 GTCTTC GAAGAC(2/6)
BsaHI 1810 GACGTC G[GA]^CG[CT]C
BsaJI 976 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 543 ACCGGT [AT]^CCGG[AT]
BsiYI 1728 CCCTTCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1728 CCCTTCGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 11 GGGAC GGGAC(10/14)
Bsp1286I 574 GAGCCC G[AGT]GC[ACT]^C
BspMI 1396 GCAGGT ACCTGC(4/8)
BstBI 746 TTCGAA TT^CGAA
BstEII 679 GGTCACC G^GT[ACGT]ACC
BstPI 679 GGTCACC G^GT[ACGT]ACC
Bsu36I 631 CCTCAGG CC^T[ACGT]AGG
EaeI 1401 TGGCCA [CT]^GGCC[GA]
Eco81I 631 CCTCAGG CC^T[ACGT]AGG
EcoO65I 679 GGTCACC G^GT[ACGT]ACC
EcoRI 712 GAATTC G^AATTC
HgaI 827 GACGC GACGC(5/10)
Hin1I 1810 GACGTC G[GA]^CG[CT]C
MluNI 1401 TGGCCA TGG^CCA
MscI 1401 TGGCCA TGG^CCA
MspA1I 1433 CAGCTG C[AC]G^C[GT]G
NdeI 1129 CATATG CA^TATG
NlaIV 72 GGAACC GG[ACGT]^[ACGT]CC
NspI 1072 GCATGC [GA]CATG^[CT]
NspV 746 TTCGAA TT^CGAA
PinAI 543 ACCGGT A^CCGGT
PstI 1394 CTGCAG CTGCA^G
PvuII 1433 CAGCTG CAG^CTG
ScaI 481 AGTACT AGT^ACT
SfuI 746 TTCGAA TT^CGAA
SmlI 169 CTTAAG C^T[CT][GA]AG
SpeI 1381 ACTAGT A^CTAGT
SphI 1072 GCATGC GCATG^C
TspRI 522 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 721 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbvI 1333 GCAGC GCAGC(8/12)
1393 GCTGC
BpmI 286 CTCCAG CTGGAG(16/14)
77 CTGGAG
BsmAI 227 GTCTC GTCTC(1/5)
284 GTCTC
BsrFI 543 ACCGGT [GA]^CCGG[CT]
1329 ACCGGC
BstYI 81 AGATCC [GA]^GATC[CT]
1975 AGATCT
Cfr10I 543 ACCGGT [GA]^CCGG[CT]
1329 ACCGGC
Cfr13I 1186 GGCCC G^G[ACGT]CC
1726 GGCCC
DraII 1185 AGGCCCT [GA]G^G[ACGT]CC[CT]
1725 AGGCCCT
EarI 229 CTCTTC CTCTTC(1/4)
1847 CTCTTC
EcoO109I 1185 AGGCCCT [GA]G^G[ACGT]CC[CT]
1725 AGGCCCT
HapII 544 CCGG C^CGG
1330 CCGG
HpaII 544 CCGG C^CGG
1330 CCGG
Ksp632I 229 CTCTTC CTCTTC(1/4)
1847 CTCTTC
MflI 81 AGATCC [GA]^GATC[CT]
1975 AGATCT
MspI 544 CCGG C^CGG
1330 CCGG
MwoI 889 GCTACAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1082 GCCTCCTTTGC
NlaIII 1073 CATG CATG^
1765 CATG
Sau96I 1186 GGCCC G^G[ACGT]CC
1726 GGCCC
SfaNI 47 GATGC GCATC(5/9)
1856 GATGC
TseI 1333 GCAGC G^C[AT]GC
1393 GCTGC
XhoII 81 AGATCC [GA]^GATC[CT]
1975 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII PleI
RsaI Sau3AI ScrFI SfcI TaqI
TfiI Tru9I Tsp45I Tsp509I TthHB8I