##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssKI       BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MroI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
StyI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          138  GTATAC               GT^[AC][GT]AC
AfaI           81  GTAC                 GT^AC
AluI          164  AGCT                 AG^CT
BalI          340  TGGCCA               TGG^CCA
BbvI          124  GCAGC                GCAGC(8/12)
BseRI          56  CTCCTC               GAGGAG(10/8)
BsiYI         326  CCGATCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          326  CCGATCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I       80  TGTACA               T^GTACA
BsrGI          80  TGTACA               T^GTACA
BsrI           25  ACTGG                ACTGG(1/-1)
BssSI          61  CACGAG               CACGAG(-5/-1)
Bst1107I      138  GTATAC               GTA^TAC
BstZ17I       138  GTATAC               GTA^TAC
Cac8I         165  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI           70  CTAAG                C^T[ACGT]AG
DpnII         328  GATC                 ^GATC
EaeI          340  TGGCCA               [CT]^GGCC[GA]
FauI            4  GCGGG                CCCGC(4/6)
Fnu4HI        124  GCAGC                GC^[ACGT]GC
HphI          237  GGTGA                GGTGA(8/7)
ItaI          124  GCAGC                GC^[ACGT]GC
MboI          328  GATC                 ^GATC
MluNI         340  TGGCCA               TGG^CCA
MscI          340  TGGCCA               TGG^CCA
NdeII         328  GATC                 ^GATC
NlaIII        256  CATG                 CATG^
RsaI           81  GTAC                 GT^AC
SapI          313  GAAGAGC              GCTCTTC(1/4)
Sau3AI        328  GATC                 ^GATC
SmlI          103  CTTGAG               C^T[CT][GA]AG
SspBI          80  TGTACA               T^GTACA
SspI           75  AATATT               AAT^ATT
TseI          124  GCAGC                G^C[AT]GC
XcmI           23  CCACTGGCCTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbsI           18  GAAGAC               GAAGAC(2/6)
              205  GAAGAC              
BpuAI          18  GAAGAC               GAAGAC(2/6)
              205  GAAGAC              
EarI          232  GAAGAG               CTCTTC(1/4)
              313  GAAGAG              
HaeIII         28  GGCC                 GG^CC
              341  GGCC                
HinfI          90  GATTC                G^A[ACGT]TC
              240  GATTC               
Ksp632I       232  GAAGAG               CTCTTC(1/4)
              313  GAAGAG              
MseI          176  TTAA                 T^TAA
              194  TTAA                
NlaIV         183  GGAACC               GG[ACGT]^[ACGT]CC
              300  GGAGCC              
TfiI           90  GATTC                G^A[AT]TC
              240  GATTC               
Tru9I         176  TTAA                 T^TAA
              194  TTAA                
Tsp509I       200  AATT                 ^AATT
              227  AATT                
TspRI          24  CACTG                CA[GC]TG[ACGT][ACGT]^
              331  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AciI        MboII       MnlI