Restriction map for
pFN21AE3158
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaHI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 391 AACGTT AA^CGTT
AcsI 485 AAATTT [GA]^AATT[CT]
AlwNI 398 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 485 AAATTT [GA]^AATT[CT]
Asp700I 1108 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 380 TGATCA T^GATCA
BfaI 992 CTAG C^TAG
BglII 684 AGATCT A^GATCT
BpmI 176 CTGGAG CTGGAG(16/14)
BsaBI 1111 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 659 ACCGGA [AT]^CCGG[AT]
BsmFI 871 GGGAC GGGAC(10/14)
BstYI 684 AGATCT [GA]^GATC[CT]
Cac8I 303 GCTTGC GC[ACGT]^[ACGT]GC
DraIII 989 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 867 CCATGG C^C[AT][AT]GG
FbaI 380 TGATCA T^GATCA
HapII 660 CCGG C^CGG
HpaII 660 CCGG C^CGG
MaeI 992 CTAG C^TAG
MaeII 392 ACGT A^CGT
MamI 1111 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 684 AGATCT [GA]^GATC[CT]
MslI 552 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 660 CCGG C^CGG
NcoI 867 CCATGG C^CATGG
PacI 496 TTAATTAA TTAAT^TAA
PflMI 397 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 179 GAGTC GAGTC(4/5)
PshAI 174 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 391 AACGTT AA^CGTT
PstI 1025 CTGCAG CTGCA^G
SbfI 1024 CCTGCAGG CCTGCA^GG
ScaI 287 AGTACT AGT^ACT
SexAI 410 ACCAGGT A^CC[AT]GGT
SfaNI 3 GCATC GCATC(5/9)
SfcI 1025 CTGCAG C^T[GA][CT]AG
SmlI 10 CTCAAG C^T[CT][GA]AG
Sse8387I 1024 CCTGCAGG CCTGCA^GG
StyI 867 CCATGG C^C[AT][AT]GG
TaqI 717 TCGA T^CGA
Tsp45I 589 GTCAC ^GT[GC]AC
TthHB8I 717 TCGA T^CGA
Van91I 397 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 684 AGATCT [GA]^GATC[CT]
XmnI 1108 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 193 AGCT AG^CT
763 AGCT
BbvI 669 GCAGC GCAGC(8/12)
764 GCTGC
BsaI 295 GAGACC GGTCTC(1/5)
573 GGTCTC
BseRI 1000 GAGGAG GAGGAG(10/8)
1057 GAGGAG
DsaI 524 CCGTGG C^C[GA][CT]GG
867 CCATGG
EcoT22I 1 ATGCAT ATGCA^T
251 ATGCAT
Fnu4HI 669 GCAGC GC^[ACGT]GC
764 GCTGC
HphI 988 TCACC GGTGA(8/7)
1123 TCACC
ItaI 669 GCAGC GC^[ACGT]GC
764 GCTGC
MaeIII 467 GTAAC ^GT[ACGT]AC
589 GTCAC
NlaIV 367 GGAGCC GG[ACGT]^[ACGT]CC
1030 GGAACC
NsiI 1 ATGCAT ATGCA^T
251 ATGCAT
NspI 253 GCATGT [GA]CATG^[CT]
874 ACATGC
TseI 669 GCAGC G^C[AT]GC
764 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI DdeI
DpnII EarI EcoRII FokI HinfI
Ksp632I MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI TfiI Tru9I Tsp509I
TspRI