Restriction map for
pFN21AB7922
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 296 GCGG CCGC(-3/-1)
AlwNI 380 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 463 ATTAAT AT^TAAT
AsnI 463 ATTAAT AT^TAAT
AspI 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 596 CCCGAG C^[CT]CG[GA]G
BsaJI 701 CCCTGG C^C[ACGT][ACGT]GG
BseRI 245 CTCCTC GAGGAG(10/8)
BsgI 76 GTGCAG GTGCAG(16/14)
BsoBI 596 CCCGAG C^[CT]CG[GA]G
BspMI 48 ACCTGC ACCTGC(4/8)
BssKI 702 CCTGG ^CC[ACGT]GG
BstNI 702 CCTGG CC^[AT]GG
BstYI 954 AGATCC [GA]^GATC[CT]
CfoI 522 GCGC GCG^C
DraI 223 TTTAAA TTT^AAA
EcoRI 768 GAATTC G^AATTC
EcoRII 702 CCTGG ^CC[AT]GG
HhaI 522 GCGC GCG^C
HinP1I 522 GCGC G^CGC
MaeII 188 ACGT A^CGT
MflI 954 AGATCC [GA]^GATC[CT]
MvaI 702 CCTGG CC^[AT]GG
MwoI 69 GCCTCTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 178 CATATG CA^TATG
PflFI 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 58 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 241 GACTC GAGTC(4/5)
PshBI 463 ATTAAT AT^TAAT
SacI 820 GAGCTC GAGCT^C
ScrFI 702 CCTGG CC^[ACGT]GG
SspI 460 AATATT AAT^ATT
Tth111I 795 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 58 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 28 CCAACTCTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 954 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 356 GTCTAC GT^[AC][GT]AC
775 GTAGAC
AfaI 561 GTAC GT^AC
683 GTAC
AspHI 731 GTGCTC G[AT]GC[AT]^C
820 GAGCTC
BanII 593 GAGCCC G[GA]GC[CT]^C
820 GAGCTC
BbvI 78 GCAGC GCAGC(8/12)
886 GCTGC
BclI 9 TGATCA T^GATCA
828 TGATCA
BsiHKAI 731 GTGCTC G[AT]GC[AT]^C
820 GAGCTC
BsmAI 411 GAGAC GTCTC(1/5)
801 GTCTC
Cfr13I 67 GGGCC G^G[ACGT]CC
808 GGGCC
FbaI 9 TGATCA T^GATCA
828 TGATCA
Fnu4HI 78 GCAGC GC^[ACGT]GC
886 GCTGC
ItaI 78 GCAGC GC^[ACGT]GC
886 GCTGC
NlaIV 535 GGAACC GG[ACGT]^[ACGT]CC
807 GGGGCC
NspI 81 GCATGC [GA]CATG^[CT]
917 GCATGC
PstI 50 CTGCAG CTGCA^G
980 CTGCAG
RsaI 561 GTAC GT^AC
683 GTAC
Sau96I 67 GGGCC G^G[ACGT]CC
808 GGGCC
SfaNI 106 GATGC GCATC(5/9)
550 GCATC
SphI 81 GCATGC GCATG^C
917 GCATGC
TseI 78 GCAGC G^C[AT]GC
886 GCTGC
Tsp45I 42 GTCAC ^GT[GC]AC
739 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BmyI BsiYI BslI Bsp1286I BstF5I
Cac8I DdeI DpnII FokI HaeIII
HinfI HphI MaeI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI SfcI TfiI Tru9I Tsp509I
TspRI