Restriction map for
pFN21AE5741
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BglI BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 919 CGCG CG^CG
AciI 1720 CCGC CCGC(-3/-1)
AfaI 1378 GTAC GT^AC
AflII 504 CTTAAG C^TTAAG
AhdI 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 415 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 922 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1102 GGACC G^G[AT]CC
BanII 967 GAGCCC G[GA]GC[CT]^C
BclI 17 TGATCA T^GATCA
BcnI 1702 CCGGG CC^[GC]GG
BfrI 504 CTTAAG C^TTAAG
BglII 1860 AGATCT A^GATCT
BlpI 423 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC
BsgI 1396 CTGCAC GTGCAG(16/14)
BsmI 1371 GAATGC GAATGC(1/-1)
BspMI 879 GCAGGT ACCTGC(4/8)
BstUI 919 CGCG CG^CG
CelII 423 GCTCAGC GC^T[ACGT]AGC
DraI 813 TTTAAA TTT^AAA
DsaI 756 CCATGG C^C[GA][CT]GG
Eam1105I 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1632 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1619 ATGCAT ATGCA^T
FbaI 17 TGATCA T^GATCA
HapII 1702 CCGG C^CGG
HgaI 435 GCGTC GACGC(5/10)
HincII 1296 GTTAAC GT[CT]^[GA]AC
HindII 1296 GTTAAC GT[CT]^[GA]AC
HpaI 1296 GTTAAC GTT^AAC
HpaII 1702 CCGG C^CGG
MfeI 349 CAATTG C^AATTG
MslI 1176 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1317 CAGCTG C[AC]G^C[GT]G
MspI 1702 CCGG C^CGG
MunI 349 CAATTG C^AATTG
MvnI 919 CGCG CG^CG
NciI 1702 CCGGG CC^[GC]GG
NcoI 756 CCATGG C^CATGG
NsiI 1619 ATGCAT ATGCA^T
NspI 953 ACATGC [GA]CATG^[CT]
PpuMI 1101 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1317 CAGCTG CAG^CTG
RsaI 1378 GTAC GT^AC
TfiI 519 GATTC G^A[AT]TC
XmnI 922 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 1210 GAATTT [GA]^AATT[CT]
1310 AAATTT
AlwI 42 GATCC GGATC(4/5)
1671 GGATC
ApoI 1210 GAATTT [GA]^AATT[CT]
1310 AAATTT
BfaI 589 CTAG C^TAG
1004 CTAG
BmyI 967 GAGCCC G[AGT]GC[ACT]^C
1129 GTGCCC
BsmFI 441 GGGAC GGGAC(10/14)
1101 GGGAC
Bsp1286I 967 GAGCCC G[AGT]GC[ACT]^C
1129 GTGCCC
BsrDI 934 GCAATG GCAATG(2/0)
1685 GCAATG
BstAPI 414 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
879 GCAGGTGGTGC
BstYI 41 AGATCC [GA]^GATC[CT]
1860 AGATCT
DraII 733 GGGGCCT [GA]G^G[ACGT]CC[CT]
1101 GGGACCC
EarI 1747 GAAGAG CTCTTC(1/4)
1843 GAAGAG
EcoO109I 733 GGGGCCT [GA]G^G[ACGT]CC[CT]
1101 GGGACCC
EcoT14I 756 CCATGG C^C[AT][AT]GG
1568 CCTTGG
Ksp632I 1747 GAAGAG CTCTTC(1/4)
1843 GAAGAG
MaeI 589 CTAG C^TAG
1004 CTAG
MflI 41 AGATCC [GA]^GATC[CT]
1860 AGATCT
PstI 112 CTGCAG CTGCA^G
1636 CTGCAG
SapI 1747 GAAGAGC GCTCTTC(1/4)
1843 GAAGAGC
SfcI 112 CTGCAG C^T[GA][CT]AG
1636 CTGCAG
SmlI 494 CTCAAG C^T[CT][GA]AG
504 CTTAAG
StyI 756 CCATGG C^C[AT][AT]GG
1568 CCTTGG
XhoII 41 AGATCC [GA]^GATC[CT]
1860 AGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AluI BalI BbsI BbvCI BbvI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I