Restriction map for
pFN21AE3371
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AccI AccIII AclI AgeI
AhdI AocI ApaI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbvCI BciVI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoT22I FspI
KpnI KspI MamI MluI MroI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflII 1747 CTTAAG C^TTAAG
AflIII 1448 ACATGT A^C[GA][CT]GT
Alw44I 502 GTGCAC G^TGCAC
ApaLI 502 GTGCAC G^TGCAC
AseI 2932 ATTAAT AT^TAAT
AsnI 2932 ATTAAT AT^TAAT
AvaI 393 CCCGGG C^[CT]CG[GA]G
BfrI 1747 CTTAAG C^TTAAG
BsaWI 386 ACCGGA [AT]^CCGG[AT]
BsiEI 1071 CGACCG CG[GA][CT]^CG
BsmI 3113 GAATGC GAATGC(1/-1)
BsoBI 393 CCCGGG C^[CT]CG[GA]G
BspLU11I 1448 ACATGT A^CATGT
BsrDI 1926 CATTGC GCAATG(2/0)
BssSI 1036 CACGAG CACGAG(-5/-1)
BstEII 540 GGTGACC G^GT[ACGT]ACC
BstPI 540 GGTGACC G^GT[ACGT]ACC
BstXI 1550 CCATTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 406 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 540 GGTGACC G^GT[ACGT]ACC
EcoRI 2325 GAATTC G^AATTC
FseI 183 GGCCGGCC GGCCGG^CC
NaeI 184 GCCGGC GCC^GGC
NcoI 101 CCATGG C^CATGG
NgoMIV 184 GCCGGC G^CCGGC
PshBI 2932 ATTAAT AT^TAAT
SacI 106 GAGCTC GAGCT^C
SmaI 393 CCCGGG CCC^GGG
SspI 2851 AATATT AAT^ATT
StuI 1331 AGGCCT AGG^CCT
XmaCI 393 CCCGGG C^CCGGG
XmaI 393 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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Aor51HI 231 AGCGCT AGC^GCT
2382 AGCGCT
Asp700I 901 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2755 GAAAAGTTTC
AspHI 106 GAGCTC G[AT]GC[AT]^C
502 GTGCAC
BbeI 56 GGCGCC GGCGC^C
724 GGCGCC
BbrPI 178 CACGTG CAC^GTG
910 CACGTG
BcgI 1577 CGATAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
656 GCATCTTCATCG
BfaI 943 CTAG C^TAG
3052 CTAG
BglII 323 AGATCT A^GATCT
1295 AGATCT
BsaAI 178 CACGTG [CT]AC^GT[GA]
910 CACGTG
BsaI 238 GAGACC GGTCTC(1/5)
702 GGTCTC
BsiHKAI 106 GAGCTC G[AT]GC[AT]^C
502 GTGCAC
BspMI 1394 ACCTGC ACCTGC(4/8)
2318 ACCTGC
EarI 901 GAAGAG CTCTTC(1/4)
961 GAAGAG
Eco47III 231 AGCGCT AGC^GCT
2382 AGCGCT
EcoNI 942 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2156 CCTATCTGAGG
EcoRV 1105 GATATC GAT^ATC
1458 GATATC
FauI 423 CCCGC CCCGC(4/6)
546 CCCGC
HgaI 2650 GACGC GACGC(5/10)
818 GCGTC
HincII 1875 GTTAAC GT[CT]^[GA]AC
3123 GTTAAC
HindII 1875 GTTAAC GT[CT]^[GA]AC
3123 GTTAAC
HpaI 1875 GTTAAC GTT^AAC
3123 GTTAAC
KasI 56 GGCGCC G^GCGCC
724 GGCGCC
Ksp632I 901 GAAGAG CTCTTC(1/4)
961 GAAGAG
MaeI 943 CTAG C^TAG
3052 CTAG
NarI 56 GGCGCC GG^CGCC
724 GGCGCC
NdeI 2357 CATATG CA^TATG
2597 CATATG
NspI 1448 ACATGT [GA]CATG^[CT]
2522 ACATGC
PflMI 264 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1824 CCAATTGTTGG
PmaCI 178 CACGTG CAC^GTG
910 CACGTG
PmlI 178 CACGTG CAC^GTG
910 CACGTG
SfoI 56 GGCGCC GGC^GCC
724 GGCGCC
Van91I 264 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1824 CCAATTGTTGG
XmnI 901 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2755 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII