Restriction map for
pFN21AE1941
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HhaI HinP1I HindIII HpaII KasI
KpnI KspI MfeI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 256 GTCTAC GT^[AC][GT]AC
AciI 10 GCGG CCGC(-3/-1)
AcyI 1302 GACGCC G[GA]^CG[CT]C
AocI 358 CCTGAGG CC^T[ACGT]AGG
AseI 1072 ATTAAT AT^TAAT
AsnI 1072 ATTAAT AT^TAAT
BanI 915 GGCACC G^G[CT][GA]CC
BciVI 468 GTATCC GTATCC(6/5)
BfaI 494 CTAG C^TAG
BpmI 1327 CTGGAG CTGGAG(16/14)
BsaBI 325 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1302 GACGCC G[GA]^CG[CT]C
BsaI 1343 GAGACC GGTCTC(1/5)
BsaJI 1224 CCTTGG C^C[ACGT][ACGT]GG
BseRI 587 GAGGAG GAGGAG(10/8)
BsmI 112 GAATGC GAATGC(1/-1)
BstAPI 664 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 358 CCTGAGG CC^T[ACGT]AGG
DraII 1244 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 358 CCTGAGG CC^T[ACGT]AGG
EcoNI 706 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1244 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1224 CCTTGG C^C[AT][AT]GG
HgaI 1302 GACGC GACGC(5/10)
Hin1I 1302 GACGCC G[GA]^CG[CT]C
HpaI 637 GTTAAC GTT^AAC
MaeI 494 CTAG C^TAG
MaeII 1235 ACGT A^CGT
MamI 325 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1441 CAGCTG C[AC]G^C[GT]G
PpuMI 1244 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1072 ATTAAT AT^TAAT
PvuII 1441 CAGCTG CAG^CTG
ScaI 203 AGTACT AGT^ACT
StyI 1224 CCTTGG C^C[AT][AT]GG
Tsp45I 1399 GTGAC ^GT[GC]AC
XbaI 493 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 489 AAATTC [GA]^AATT[CT]
1314 GAATTT
AlwNI 812 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1250 CAGTTGCTG
ApoI 489 AAATTC [GA]^AATT[CT]
1314 GAATTT
AvaII 1245 GGACC G^G[AT]CC
1271 GGTCC
BglII 879 AGATCT A^GATCT
1176 AGATCT
Cac8I 610 GCTGGC GC[ACGT]^[ACGT]GC
1305 GCCAGC
EarI 391 CTCTTC CTCTTC(1/4)
594 CTCTTC
HincII 637 GTTAAC GT[CT]^[GA]AC
1167 GTTGAC
HindII 637 GTTAAC GT[CT]^[GA]AC
1167 GTTGAC
Ksp632I 391 CTCTTC CTCTTC(1/4)
594 CTCTTC
MwoI 10 GCGGCAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
664 GCAGGACTTGC
NlaIV 915 GGCACC GG[ACGT]^[ACGT]CC
1245 GGACCC
PleI 46 GACTC GAGTC(4/5)
590 GAGTC
SfcI 582 CTACAG C^T[GA][CT]AG
1159 CTACAG
SmlI 313 CTTGAG C^T[CT][GA]AG
1348 CTTGAG
TfiI 3 GAATC G^A[AT]TC
937 GATTC
XcmI 415 CCAGAGTGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
600 CCAAGAGACTGCTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI BalI BbsI
BbvI BpuAI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MvaI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI SspI Tru9I TseI
Tsp509I TspRI XhoII