Restriction map for
pFN21AE3047
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI Fnu4HI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
ItaI KasI KpnI KspI MaeII
MamI MfeI MluI MslI MspA1I
MunI MvnI MwoI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TseI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 470 TCCGGA T^CCGGA
AciI 136 CCGC CCGC(-3/-1)
Alw44I 436 GTGCAC G^TGCAC
AlwNI 374 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 460 CCTGAGG CC^T[ACGT]AGG
ApaLI 436 GTGCAC G^TGCAC
AspHI 436 GTGCAC G[AT]GC[AT]^C
BalI 56 TGGCCA TGG^CCA
BamHI 116 GGATCC G^GATCC
BbsI 259 GTCTTC GAAGAC(2/6)
BfaI 121 CTAG C^TAG
BmyI 436 GTGCAC G[AGT]GC[ACT]^C
BpmI 380 CTGGAG CTGGAG(16/14)
BpuAI 259 GTCTTC GAAGAC(2/6)
BsaJI 105 CCAAGG C^C[ACGT][ACGT]GG
BseAI 470 TCCGGA T^CCGGA
BsiHKAI 436 GTGCAC G[AT]GC[AT]^C
BsmAI 527 GAGAC GTCTC(1/5)
BsmFI 185 GGGAC GGGAC(10/14)
Bsp1286I 436 GTGCAC G[AGT]GC[ACT]^C
BspEI 470 TCCGGA T^CCGGA
BsrFI 222 GCCGGC [GA]^CCGG[CT]
Bsu36I 460 CCTGAGG CC^T[ACGT]AGG
Cfr10I 222 GCCGGC [GA]^CCGG[CT]
DraI 492 TTTAAA TTT^AAA
DraII 27 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 342 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 19 CTCTTC CTCTTC(1/4)
Eco81I 460 CCTGAGG CC^T[ACGT]AGG
EcoNI 228 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 27 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 105 CCAAGG C^C[AT][AT]GG
FauI 135 CCCGC CCCGC(4/6)
FseI 221 GGCCGGCC GGCCGG^CC
HphI 252 GGTGA GGTGA(8/7)
Ksp632I 19 CTCTTC CTCTTC(1/4)
MaeI 121 CTAG C^TAG
MaeIII 142 GTTAC ^GT[ACGT]AC
MluNI 56 TGGCCA TGG^CCA
MroI 470 TCCGGA T^CCGGA
MscI 56 TGGCCA TGG^CCA
MseI 493 TTAA T^TAA
NaeI 222 GCCGGC GCC^GGC
NgoMIV 222 GCCGGC G^CCGGC
NspI 138 GCATGT [GA]CATG^[CT]
PflMI 373 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 211 GAGTC GAGTC(4/5)
StyI 105 CCAAGG C^C[AT][AT]GG
TaqI 176 TCGA T^CGA
TfiI 73 GAATC G^A[AT]TC
Tru9I 493 TTAA T^TAA
TthHB8I 176 TCGA T^CGA
Van91I 373 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BsaWI 284 ACCGGA [AT]^CCGG[AT]
470 TCCGGA
BseRI 463 GAGGAG GAGGAG(10/8)
535 GAGGAG
BssKI 373 CCAGG ^CC[ACGT]GG
447 CCTGG
BstNI 373 CCAGG CC^[AT]GG
447 CCTGG
Cac8I 218 GCTGGC GC[ACGT]^[ACGT]GC
222 GCCGGC
Cfr13I 28 GGGCC G^G[ACGT]CC
225 GGCCC
EaeI 56 TGGCCA [CT]^GGCC[GA]
220 TGGCCG
EcoRI 172 GAATTC G^AATTC
478 GAATTC
EcoRII 373 CCAGG ^CC[AT]GG
447 CCTGG
HinfI 73 GAATC G^A[ACGT]TC
211 GAGTC
MvaI 373 CCAGG CC^[AT]GG
447 CCTGG
NlaIV 27 GGGGCC GG[ACGT]^[ACGT]CC
116 GGATCC
Sau96I 28 GGGCC G^G[ACGT]CC
225 GGCCC
ScrFI 373 CCAGG CC^[ACGT]GG
447 CCTGG
SfaNI 199 GATGC GCATC(5/9)
238 GATGC
TspRI 87 CACTG CA[GC]TG[ACGT][ACGT]^
346 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI AlwI ApoI BsiYI
BslI BstF5I BstYI DdeI DpnII
FokI HaeIII HapII HpaII MboI
MboII MflI MnlI MspI NdeII
NlaIII Sau3AI Tsp509I XhoII