Restriction map for
pFN21AE5165
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI HphI KasI KpnI
Ksp632I MaeI MaeII MamI MfeI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 191 ACGCGT A^C[GA][CT]GT
AlwNI 157 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 63 CCTTAGG CC^T[ACGT]AGG
ApaI 176 GGGCCC GGGCC^C
AspHI 195 GTGCTC G[AT]GC[AT]^C
AvaI 179 CCCGGG C^[CT]CG[GA]G
AvaII 281 GGTCC G^G[AT]CC
BalI 227 TGGCCA TGG^CCA
BglI 139 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 203 AGATCT A^GATCT
BpmI 331 CTGGAG CTGGAG(16/14)
BsiHKAI 195 GTGCTC G[AT]GC[AT]^C
BsiYI 344 CCGACACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 344 CCGACACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 179 CCCGGG C^[CT]CG[GA]G
BspMI 288 GCAGGT ACCTGC(4/8)
BstNI 225 CCTGG CC^[AT]GG
Bsu36I 63 CCTTAGG CC^T[ACGT]AGG
DrdI 106 GACATTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 227 TGGCCA [CT]^GGCC[GA]
Eco81I 63 CCTTAGG CC^T[ACGT]AGG
EcoRII 225 CCTGG ^CC[AT]GG
HincII 115 GTCAAC GT[CT]^[GA]AC
HindII 115 GTCAAC GT[CT]^[GA]AC
KspI 21 CCGCGG CCGC^GG
MaeIII 409 GTCAC ^GT[ACGT]AC
MluI 191 ACGCGT A^CGCGT
MluNI 227 TGGCCA TGG^CCA
MscI 227 TGGCCA TGG^CCA
MseI 134 TTAA T^TAA
MvaI 225 CCTGG CC^[AT]GG
NlaIII 120 CATG CATG^
PpuMI 280 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 21 CCGCGG CCGC^GG
SfiI 138 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 179 CCCGGG CCC^GGG
Tru9I 134 TTAA T^TAA
Tsp45I 409 GTCAC ^GT[GC]AC
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BamHI 45 GGATCC G^GATCC
368 GGATCC
BanII 16 GAGCCC G[GA]GC[CT]^C
176 GGGCCC
BbsI 123 GAAGAC GAAGAC(2/6)
400 GTCTTC
BpuAI 123 GAAGAC GAAGAC(2/6)
400 GTCTTC
BsaI 96 GAGACC GGTCTC(1/5)
340 GAGACC
BsmAI 96 GAGAC GTCTC(1/5)
340 GAGAC
BsrI 42 ACTGG ACTGG(1/-1)
49 CCAGT
BstF5I 387 CATCC GGATG(2/0)
393 CATCC
CfoI 239 GCGC GCG^C
253 GCGC
DraII 175 GGGGCCC [GA]G^G[ACGT]CC[CT]
280 GGGTCCT
DsaI 21 CCGCGG C^C[GA][CT]GG
311 CCGTGG
EcoO109I 175 GGGGCCC [GA]G^G[ACGT]CC[CT]
280 GGGTCCT
FokI 387 CATCC GGATG(9/13)
393 CATCC
HapII 93 CCGG C^CGG
180 CCGG
HhaI 239 GCGC GCG^C
253 GCGC
HinP1I 239 GCGC G^CGC
253 GCGC
HinfI 363 GACTC G^A[ACGT]TC
398 GAGTC
HpaII 93 CCGG C^CGG
180 CCGG
MboII 123 GAAGA GAAGA(8/7)
401 TCTTC
MspI 93 CCGG C^CGG
180 CCGG
PleI 363 GACTC GAGTC(4/5)
398 GAGTC
PstI 256 CTGCAG CTGCA^G
262 CTGCAG
PvuII 142 CAGCTG CAG^CTG
259 CAGCTG
SfcI 256 CTGCAG C^T[GA][CT]AG
262 CTGCAG
TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^
359 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BmyI BsaJI Bsp1286I BssKI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII FauI Fnu4HI HaeIII ItaI
MboI MflI MnlI MspA1I MvnI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TseI XhoII