##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DraI        DraII       
DraIII      EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NarI        NciI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AfaI          891  GTAC                 GT^AC
AhdI          303  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         303  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI           62  CTAG                 C^TAG
BsaI          883  GAGACC               GGTCTC(1/5)
BsmBI         213  GAGACG               CGTCTC(1/5)
BspHI         626  TCATGA               T^CATGA
BspMI         341  ACCTGC               ACCTGC(4/8)
BsrBI         318  GAGCGG               CCGCTC(-3/-3)
BstF5I        789  GGATG                GGATG(2/0)
DrdI          793  GACGGCATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      303  GACAGAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          315  GAAGAG               CTCTTC(1/4)
FokI          789  GGATG                GGATG(9/13)
FseI            4  GGCCGGCC             GGCCGG^CC
HgaI          554  GACGC                GACGC(5/10)
Ksp632I       315  GAAGAG               CTCTTC(1/4)
MaeI           62  CTAG                 C^TAG
MaeII         197  ACGT                 A^CGT
MboII         315  GAAGA                GAAGA(8/7)
MseI          901  TTAA                 T^TAA
MspA1I        679  CAGCGG               C[AC]G^C[GT]G
NaeI            5  GCCGGC               GCC^GGC
NcoI          131  CCATGG               C^CATGG
NdeI          734  CATATG               CA^TATG
NgoMIV          5  GCCGGC               G^CCGGC
PleI          562  GACTC                GAGTC(4/5)
RcaI          626  TCATGA               T^CATGA
RsaI          891  GTAC                 GT^AC
SacI          396  GAGCTC               GAGCT^C
SapI          315  GAAGAGC              GCTCTTC(1/4)
SfaNI         797  GCATC                GCATC(5/9)
SmlI          865  CTCAAG               C^T[CT][GA]AG
TaqI          425  TCGA                 T^CGA
TfiI          623  GAATC                G^A[AT]TC
Tru9I         901  TTAA                 T^TAA
TthHB8I       425  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwNI          74  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              602  CAGTGGCTG           
BanII         396  GAGCTC               G[GA]GC[CT]^C
              870  GAGCCC              
BsrI          299  ACTGG                ACTGG(1/-1)
              357  CCAGT               
Cac8I           5  GCCGGC               GC[ACGT]^[ACGT]GC
               45  GCCAGC              
DsaI          131  CCATGG               C^C[GA][CT]GG
              228  CCACGG              
EcoT14I       131  CCATGG               C^C[AT][AT]GG
              785  CCAAGG              
HinfI         562  GACTC                G^A[ACGT]TC
              623  GAATC               
HphI          446  TCACC                GGTGA(8/7)
              835  TCACC               
PstI           55  CTGCAG               CTGCA^G
              343  CTGCAG              
StyI          131  CCATGG               C^C[AT][AT]GG
              785  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        AspHI       
AvaII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrFI       BssKI       BstNI       
BstYI       Bsu36I      Cfr10I      Cfr13I      DdeI        
DpnII       Eco81I      EcoRII      Fnu4HI      HaeIII      
HapII       HpaII       ItaI        MaeIII      MboI        
MflI        MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfcI        TseI        Tsp45I      TspRI       
XhoII