Restriction map for
pFN21AE6459
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbeI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
BstAPI BstBI BstEII BstPI ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV FbaI FseI
HaeII HincII HindII HindIII HpaI
KasI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NspI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI PstI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1141 GACGTC GACGT^C
Acc65I 848 GGTACC G^GTACC
AccI 325 GTATAC GT^[AC][GT]AC
AccII 61 CGCG CG^CG
AclI 217 AACGTT AA^CGTT
AflIII 1241 ACACGT A^C[GA][CT]GT
AlwNI 1033 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 848 GGTACC G^GTACC
AviII 677 TGCGCA TGC^GCA
BalI 257 TGGCCA TGG^CCA
BamHI 607 GGATCC G^GATCC
BanI 848 GGTACC G^G[CT][GA]CC
BbrPI 1242 CACGTG CAC^GTG
BsaAI 1242 CACGTG [CT]AC^GT[GA]
BsaWI 101 ACCGGA [AT]^CCGG[AT]
BseRI 1193 CTCCTC GAGGAG(10/8)
BsiEI 75 CGGCCG CG[GA][CT]^CG
BsmBI 125 GAGACG CGTCTC(1/5)
Bsp1407I 363 TGTACA T^GTACA
BsrFI 926 GCCGGC [GA]^CCGG[CT]
BsrGI 363 TGTACA T^GTACA
Bst1107I 325 GTATAC GTA^TAC
BstUI 61 CGCG CG^CG
BstXI 260 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 607 GGATCC [GA]^GATC[CT]
BstZ17I 325 GTATAC GTA^TAC
CfoI 678 GCGC GCG^C
Cfr10I 926 GCCGGC [GA]^CCGG[CT]
DrdI 1339 GACGCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 75 CGGCCG C^GGCCG
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
EcoT22I 145 ATGCAT ATGCA^T
FspI 677 TGCGCA TGC^GCA
HgaI 1339 GACGC GACGC(5/10)
HhaI 678 GCGC GCG^C
HinP1I 678 GCGC G^CGC
KpnI 848 GGTACC GGTAC^C
KspI 60 CCGCGG CCGC^GG
MflI 607 GGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MvnI 61 CGCG CG^CG
NaeI 926 GCCGGC GCC^GGC
NgoMIV 926 GCCGGC G^CCGGC
NsiI 145 ATGCAT ATGCA^T
PaeR7I 1010 CTCGAG C^TCGAG
PmaCI 1242 CACGTG CAC^GTG
PmlI 1242 CACGTG CAC^GTG
Psp1406I 217 AACGTT AA^CGTT
SacI 91 GAGCTC GAGCT^C
SacII 60 CCGCGG CCGC^GG
SspBI 363 TGTACA T^GTACA
StuI 1125 AGGCCT AGG^CCT
XhoI 1010 CTCGAG C^TCGAG
XhoII 607 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 393 GAATTT [GA]^AATT[CT]
787 GAATTT
AcyI 1141 GACGTC G[GA]^CG[CT]C
1339 GACGCC
AocI 823 CCTCAGG CC^T[ACGT]AGG
1269 CCTCAGG
ApaI 1150 GGGCCC GGGCC^C
1206 GGGCCC
ApoI 393 GAATTT [GA]^AATT[CT]
787 GAATTT
AspHI 91 GAGCTC G[AT]GC[AT]^C
1245 GTGCTC
AvaI 1010 CTCGAG C^[CT]CG[GA]G
1097 CTCGGG
BbvCI 444 CCTCAGC CCTCAGC(-5/-2)
387 GCTGAGG
BfaI 238 CTAG C^TAG
662 CTAG
BlpI 1038 GCTGAGC GC^T[ACGT]AGC
1060 GCTGAGC
Bpu1102I 1038 GCTGAGC GC^T[ACGT]AGC
1060 GCTGAGC
BsaHI 1141 GACGTC G[GA]^CG[CT]C
1339 GACGCC
BsiHKAI 91 GAGCTC G[AT]GC[AT]^C
1245 GTGCTC
BsoBI 1010 CTCGAG C^[CT]CG[GA]G
1097 CTCGGG
BspMI 959 ACCTGC ACCTGC(4/8)
1091 ACCTGC
BsrBI 40 CCGCTC CCGCTC(-3/-3)
877 CCGCTC
BsrDI 311 GCAATG GCAATG(2/0)
1295 GCAATG
BsrI 527 CCAGT ACTGG(1/-1)
1214 CCAGT
Bsu36I 823 CCTCAGG CC^T[ACGT]AGG
1269 CCTCAGG
CelII 1038 GCTGAGC GC^T[ACGT]AGC
1060 GCTGAGC
DpnII 608 GATC ^GATC
1356 GATC
Eco81I 823 CCTCAGG CC^T[ACGT]AGG
1269 CCTCAGG
Hin1I 1141 GACGTC G[GA]^CG[CT]C
1339 GACGCC
HphI 150 TCACC GGTGA(8/7)
874 TCACC
MaeI 238 CTAG C^TAG
662 CTAG
MaeIII 202 GTCAC ^GT[ACGT]AC
956 GTGAC
MboI 608 GATC ^GATC
1356 GATC
MslI 148 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
378 CACGGAGATG
MspA1I 60 CCGCGG C[AC]G^C[GT]G
183 CAGCGG
NcoI 153 CCATGG C^CATGG
1170 CCATGG
NdeII 608 GATC ^GATC
1356 GATC
PleI 130 GAGTC GAGTC(4/5)
793 GAGTC
SapI 93 GCTCTTC GCTCTTC(1/4)
891 GCTCTTC
Sau3AI 608 GATC ^GATC
1356 GATC
SmlI 646 CTCAAG C^T[CT][GA]AG
1010 CTCGAG
Tsp45I 202 GTCAC ^GT[GC]AC
956 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I