Restriction map for
pFN21AE5679
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI ItaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TseI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 519 ACACGT A^C[GA][CT]GT
ApaI 118 GGGCCC GGGCC^C
AspHI 880 GAGCTC G[AT]GC[AT]^C
BalI 962 TGGCCA TGG^CCA
BanI 1026 GGCACC G^G[CT][GA]CC
BbsI 301 GTCTTC GAAGAC(2/6)
BbvCI 361 GCTGAGG CCTCAGC(-5/-2)
BglI 318 GCCTTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 877 CTGGAG CTGGAG(16/14)
BpuAI 301 GTCTTC GAAGAC(2/6)
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BseRI 61 CTCCTC GAGGAG(10/8)
BsgI 991 GTGCAG GTGCAG(16/14)
BsiHKAI 880 GAGCTC G[AT]GC[AT]^C
BsmFI 436 GTCCC GGGAC(10/14)
BspHI 1206 TCATGA T^CATGA
BstAPI 984 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 799 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 23 CCGTGG C^C[GA][CT]GG
EarI 378 GAAGAG CTCTTC(1/4)
FauI 483 GCGGG CCCGC(4/6)
HapII 312 CCGG C^CGG
HincII 230 GTCAAC GT[CT]^[GA]AC
HindII 230 GTCAAC GT[CT]^[GA]AC
HpaII 312 CCGG C^CGG
Ksp632I 378 GAAGAG CTCTTC(1/4)
MaeIII 508 GTCAC ^GT[ACGT]AC
MluNI 962 TGGCCA TGG^CCA
MscI 962 TGGCCA TGG^CCA
MspI 312 CCGG C^CGG
PvuII 817 CAGCTG CAG^CTG
RcaI 1206 TCATGA T^CATGA
SacI 880 GAGCTC GAGCT^C
ScaI 1217 AGTACT AGT^ACT
SfaNI 575 GCATC GCATC(5/9)
SfiI 317 GGCCTTTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 316 AGGCCT AGG^CCT
TaqI 578 TCGA T^CGA
TfiI 1185 GATTC G^A[AT]TC
Tsp45I 508 GTCAC ^GT[GC]AC
TthHB8I 578 TCGA T^CGA
XcmI 1166 CCAAAGGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 416 GCGG CCGC(-3/-1)
483 GCGG
AcsI 110 GAATTC [GA]^AATT[CT]
837 GAATTC
AfaI 83 GTAC GT^AC
1218 GTAC
AlwNI 1226 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1317 CAGAAACTG
ApoI 110 GAATTC [GA]^AATT[CT]
837 GAATTC
AvaII 435 GGTCC G^G[AT]CC
1312 GGACC
BanII 118 GGGCCC G[GA]GC[CT]^C
880 GAGCTC
BclI 266 TGATCA T^GATCA
548 TGATCA
BmyI 118 GGGCCC G[AGT]GC[ACT]^C
880 GAGCTC
BsaI 853 GAGACC GGTCTC(1/5)
1492 GAGACC
BsaJI 23 CCGTGG C^C[ACGT][ACGT]GG
115 CCAGGG
Bsp1286I 118 GGGCCC G[AGT]GC[ACT]^C
880 GAGCTC
BsrDI 392 CATTGC GCAATG(2/0)
444 CATTGC
BssSI 927 CACGAG CACGAG(-5/-1)
1345 CTCGTG
BstXI 1166 CCAAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1213 CCAAAGTACTGG
DraII 117 AGGGCCC [GA]G^G[ACGT]CC[CT]
529 GGGGCCT
EaeI 20 TGGCCG [CT]^GGCC[GA]
962 TGGCCA
EcoO109I 117 AGGGCCC [GA]G^G[ACGT]CC[CT]
529 GGGGCCT
EcoRI 110 GAATTC G^AATTC
837 GAATTC
FbaI 266 TGATCA T^GATCA
548 TGATCA
HindIII 103 AAGCTT A^AGCTT
625 AAGCTT
MaeII 175 ACGT A^CGT
521 ACGT
MspA1I 414 CAGCGG C[AC]G^C[GT]G
817 CAGCTG
MwoI 318 GCCTTTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
984 GCAGAAAGTGC
RsaI 83 GTAC GT^AC
1218 GTAC
SmlI 424 CTCAAG C^T[CT][GA]AG
1117 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BfaI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MaeI MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI Tru9I Tsp509I TspRI XhoII