Restriction map for
pFN21AE4341
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstXI CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI Tsp45I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 457 GTATAC GT^[AC][GT]AC
AclI 446 AACGTT AA^CGTT
AocI 883 CCTGAGG CC^T[ACGT]AGG
Asp700I 904 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 92 CTCGAG C^[CT]CG[GA]G
BalI 644 TGGCCA TGG^CCA
BanII 1057 GAGCTC G[GA]GC[CT]^C
BbvI 718 GCAGC GCAGC(8/12)
BsaI 542 GAGACC GGTCTC(1/5)
BsmI 84 GAATGC GAATGC(1/-1)
BsoBI 92 CTCGAG C^[CT]CG[GA]G
BssSI 10 CTCGTG CACGAG(-5/-1)
Bst1107I 457 GTATAC GTA^TAC
BstZ17I 457 GTATAC GTA^TAC
Bsu36I 883 CCTGAGG CC^T[ACGT]AGG
DraI 427 TTTAAA TTT^AAA
DraII 887 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1008 CCATGG C^C[GA][CT]GG
EaeI 644 TGGCCA [CT]^GGCC[GA]
Eco81I 883 CCTGAGG CC^T[ACGT]AGG
EcoO109I 887 AGGACCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 718 GCAGC GC^[ACGT]GC
HaeII 15 GGCGCT [GA]GCGC^[CT]
HinfI 393 GAATC G^A[ACGT]TC
ItaI 718 GCAGC GC^[ACGT]GC
MaeII 447 ACGT A^CGT
MluNI 644 TGGCCA TGG^CCA
MscI 644 TGGCCA TGG^CCA
NcoI 1008 CCATGG C^CATGG
NspI 218 GCATGC [GA]CATG^[CT]
PaeR7I 92 CTCGAG C^TCGAG
PpuMI 887 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 446 AACGTT AA^CGTT
SacI 1057 GAGCTC GAGCT^C
ScaI 906 AGTACT AGT^ACT
SfcI 618 CTACAG C^T[GA][CT]AG
SmlI 92 CTCGAG C^T[CT][GA]AG
SphI 218 GCATGC GCATG^C
SwaI 426 ATTTAAAT ATTT^AAAT
TfiI 393 GAATC G^A[AT]TC
TseI 718 GCAGC G^C[AT]GC
XhoI 92 CTCGAG C^TCGAG
XmnI 904 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 430 AAATTT [GA]^AATT[CT]
1016 AAATTC
ApoI 430 AAATTT [GA]^AATT[CT]
1016 AAATTC
AspHI 948 GAGCAC G[AT]GC[AT]^C
1057 GAGCTC
BfaI 176 CTAG C^TAG
298 CTAG
BpmI 195 CTCCAG CTGGAG(16/14)
893 CTCCAG
BsiHKAI 948 GAGCAC G[AT]GC[AT]^C
1057 GAGCTC
BstYI 264 GGATCT [GA]^GATC[CT]
591 GGATCT
Cac8I 183 GCTGGC GC[ACGT]^[ACGT]GC
218 GCATGC
CfoI 16 GCGC GCG^C
232 GCGC
EcoNI 129 CCTTATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
820 CCTGCCACAGG
HaeIII 37 GGCC GG^CC
645 GGCC
HhaI 16 GCGC GCG^C
232 GCGC
HinP1I 16 GCGC G^CGC
232 GCGC
MaeI 176 CTAG C^TAG
298 CTAG
MflI 264 GGATCT [GA]^GATC[CT]
591 GGATCT
MslI 792 CACCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
987 CACCTCTGTG
SfaNI 845 GATGC GCATC(5/9)
914 GCATC
XhoII 264 GGATCT [GA]^GATC[CT]
591 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI AvaII BanI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
FokI HphI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StyI TaqI Tru9I Tsp509I TspRI
TthHB8I