Restriction map for
pFN21AB3407
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BstEII BstPI BstUI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I FauI FseI
FspI HincII HindII HpaI KasI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1786 GACGTC GACGT^C
Acc65I 2775 GGTACC G^GTACC
AcyI 1786 GACGTC G[GA]^CG[CT]C
AflIII 625 ACACGT A^C[GA][CT]GT
Alw44I 493 GTGCAC G^TGCAC
ApaI 1583 GGGCCC GGGCC^C
ApaLI 493 GTGCAC G^TGCAC
Asp718I 2775 GGTACC G^GTACC
BciVI 3618 GGATAC GTATCC(6/5)
BclI 3029 TGATCA T^GATCA
BcnI 3411 CCGGG CC^[GC]GG
BsaHI 1786 GACGTC G[GA]^CG[CT]C
BsgI 1249 GTGCAG GTGCAG(16/14)
BsiEI 3139 CGACCG CG[GA][CT]^CG
BsmI 3714 GAATGC GAATGC(1/-1)
BsrBI 3250 GAGCGG CCGCTC(-3/-3)
BssSI 3560 CTCGTG CACGAG(-5/-1)
Bst1107I 2072 GTATAC GTA^TAC
BstAPI 922 GCATCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1134 TTCGAA TT^CGAA
BstZ17I 2072 GTATAC GTA^TAC
DraI 49 TTTAAA TTT^AAA
EcoNI 2087 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1177 GAATTC G^AATTC
EcoT22I 920 ATGCAT ATGCA^T
FbaI 3029 TGATCA T^GATCA
HgaI 3192 GACGC GACGC(5/10)
Hin1I 1786 GACGTC G[GA]^CG[CT]C
HindIII 2041 AAGCTT A^AGCTT
KpnI 2775 GGTACC GGTAC^C
MfeI 2588 CAATTG C^AATTG
MunI 2588 CAATTG C^AATTG
NciI 3411 CCGGG CC^[GC]GG
NdeI 2752 CATATG CA^TATG
NsiI 920 ATGCAT ATGCA^T
NspI 3462 ACATGC [GA]CATG^[CT]
NspV 1134 TTCGAA TT^CGAA
PstI 3372 CTGCAG CTGCA^G
ScaI 990 AGTACT AGT^ACT
SexAI 334 ACCAGGT A^CC[AT]GGT
SfuI 1134 TTCGAA TT^CGAA
SmlI 2468 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1767 GTCTAC GT^[AC][GT]AC
2072 GTATAC
AseI 544 ATTAAT AT^TAAT
1014 ATTAAT
AsnI 544 ATTAAT AT^TAAT
1014 ATTAAT
Asp700I 1456 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2288 GAATTATTTC
BalI 656 TGGCCA TGG^CCA
3534 TGGCCA
BanII 1583 GGGCCC G[GA]GC[CT]^C
3355 GAGCCC
BbrPI 626 CACGTG CAC^GTG
1625 CACGTG
BfaI 2278 CTAG C^TAG
2668 CTAG
BglII 258 AGATCT A^GATCT
1223 AGATCT
BsaAI 626 CACGTG [CT]AC^GT[GA]
1625 CACGTG
BsaWI 2870 TCCGGT [AT]^CCGG[AT]
3141 ACCGGA
BseRI 26 GAGGAG GAGGAG(10/8)
1076 GAGGAG
BspMI 3676 ACCTGC ACCTGC(4/8)
2772 GCAGGT
CfoI 2675 GCGC GCG^C
3834 GCGC
DraII 1232 AGGGCCT [GA]G^G[ACGT]CC[CT]
1582 GGGGCCC
EcoO109I 1232 AGGGCCT [GA]G^G[ACGT]CC[CT]
1582 GGGGCCC
EcoRV 229 GATATC GAT^ATC
289 GATATC
HaeII 2674 GGCGCT [GA]GCGC^[CT]
3833 AGCGCC
HhaI 2675 GCGC GCG^C
3834 GCGC
HinP1I 2675 GCGC G^CGC
3834 GCGC
MaeI 2278 CTAG C^TAG
2668 CTAG
MluNI 656 TGGCCA TGG^CCA
3534 TGGCCA
MscI 656 TGGCCA TGG^CCA
3534 TGGCCA
PflMI 3189 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3240 CCAGCTCCTGG
PmaCI 626 CACGTG CAC^GTG
1625 CACGTG
PmlI 626 CACGTG CAC^GTG
1625 CACGTG
PshBI 544 ATTAAT AT^TAAT
1014 ATTAAT
SapI 1296 GAAGAGC GCTCTTC(1/4)
3225 GAAGAGC
StuI 2166 AGGCCT AGG^CCT
3072 AGGCCT
Van91I 3189 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3240 CCAGCTCCTGG
XmnI 1456 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2288 GAATTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII