Restriction map for
pFN21AE1774
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BanII
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BseRI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MluNI MscI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 565 GTAGAC GT^[AC][GT]AC
AccII 10 CGCG CG^CG
AccIII 21 TCCGGA T^CCGGA
AcsI 595 AAATTC [GA]^AATT[CT]
ApoI 595 AAATTC [GA]^AATT[CT]
AvaI 470 CCCGAG C^[CT]CG[GA]G
BamHI 466 GGATCC G^GATCC
BanI 779 GGCGCC G^G[CT][GA]CC
BbeI 779 GGCGCC GGCGC^C
BclI 518 TGATCA T^GATCA
BglI 532 GCCACAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 223 GGGCAC G[AGT]GC[ACT]^C
BpmI 757 CTGGAG CTGGAG(16/14)
BsaBI 96 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 293 GAGACC GGTCTC(1/5)
BseAI 21 TCCGGA T^CCGGA
BsgI 373 GTGCAG GTGCAG(16/14)
BsmFI 6 GTCCC GGGAC(10/14)
BsoBI 470 CCCGAG C^[CT]CG[GA]G
Bsp1286I 223 GGGCAC G[AGT]GC[ACT]^C
BspEI 21 TCCGGA T^CCGGA
BsrBI 769 GAGCGG CCGCTC(-3/-3)
BstUI 10 CGCG CG^CG
BstYI 466 GGATCC [GA]^GATC[CT]
DsaI 9 CCGCGG C^C[GA][CT]GG
EarI 738 GAAGAG CTCTTC(1/4)
FbaI 518 TGATCA T^GATCA
KasI 779 GGCGCC G^GCGCC
Ksp632I 738 GAAGAG CTCTTC(1/4)
KspI 9 CCGCGG CCGC^GG
MaeII 77 ACGT A^CGT
MaeIII 164 GTTAC ^GT[ACGT]AC
MamI 96 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 466 GGATCC [GA]^GATC[CT]
MroI 21 TCCGGA T^CCGGA
MseI 709 TTAA T^TAA
MvnI 10 CGCG CG^CG
NaeI 14 GCCGGC GCC^GGC
NarI 779 GGCGCC GG^CGCC
NgoMIV 14 GCCGGC G^CCGGC
PstI 481 CTGCAG CTGCA^G
PvuII 484 CAGCTG CAG^CTG
SacII 9 CCGCGG CCGC^GG
SfcI 481 CTGCAG C^T[GA][CT]AG
SfoI 779 GGCGCC GGC^GCC
Tru9I 709 TTAA T^TAA
Tsp509I 596 AATT ^AATT
XhoII 466 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 467 GATCC GGATC(4/5)
466 GGATC
AvaII 34 GGACC G^G[AT]CC
276 GGACC
BbvI 483 GCAGC GCAGC(8/12)
300 GCTGC
BcnI 68 CCCGG CC^[GC]GG
462 CCGGG
BsaWI 21 TCCGGA [AT]^CCGG[AT]
550 TCCGGT
BsiEI 12 CGGCCG CG[GA][CT]^CG
819 CGGCCG
BsmAI 58 GAGAC GTCTC(1/5)
293 GAGAC
BspMI 167 ACCTGC ACCTGC(4/8)
296 ACCTGC
BsrFI 14 GCCGGC [GA]^CCGG[CT]
817 ACCGGC
BsrI 190 ACTGG ACTGG(1/-1)
810 CCAGT
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
817 ACCGGC
DpnII 467 GATC ^GATC
519 GATC
EaeI 12 CGGCCG [CT]^GGCC[GA]
819 CGGCCG
EagI 12 CGGCCG C^GGCCG
819 CGGCCG
EclXI 12 CGGCCG C^GGCCG
819 CGGCCG
Eco52I 12 CGGCCG C^GGCCG
819 CGGCCG
EcoRV 100 GATATC GAT^ATC
244 GATATC
HaeII 419 AGCGCC [GA]GCGC^[CT]
779 GGCGCC
HgaI 4 GCGTC GACGC(5/10)
18 GCGTC
HinfI 679 GAGTC G^A[ACGT]TC
741 GAGTC
HphI 732 GGTGA GGTGA(8/7)
689 TCACC
MboI 467 GATC ^GATC
519 GATC
MboII 738 GAAGA GAAGA(8/7)
762 GAAGA
MspA1I 9 CCGCGG C[AC]G^C[GT]G
484 CAGCTG
NciI 68 CCCGG CC^[GC]GG
462 CCGGG
NdeII 467 GATC ^GATC
519 GATC
PleI 679 GAGTC GAGTC(4/5)
741 GAGTC
Sau3AI 467 GATC ^GATC
519 GATC
SfaNI 323 GCATC GCATC(5/9)
356 GCATC
TaqI 625 TCGA T^CGA
718 TCGA
TseI 300 GCTGC G^C[AT]GC
483 GCAGC
TthHB8I 625 TCGA T^CGA
718 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcyI AfaI AluI BsaHI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI MnlI MspI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
TspRI