Restriction map for
pFN21AE5241
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MroI MslI MspA1I MunI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI SexAI SfaNI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 742 AAATTT [GA]^AATT[CT]
Aor51HI 74 AGCGCT AGC^GCT
ApoI 742 AAATTT [GA]^AATT[CT]
Asp700I 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 506 GTGCTC G[AT]GC[AT]^C
AvaI 639 CCCGGG C^[CT]CG[GA]G
BalI 606 TGGCCA TGG^CCA
BbvI 644 GCTGC GCAGC(8/12)
BclI 578 TGATCA T^GATCA
BglII 207 AGATCT A^GATCT
BmyI 506 GTGCTC G[AGT]GC[ACT]^C
BpmI 310 CTGGAG CTGGAG(16/14)
BsaI 375 GAGACC GGTCTC(1/5)
BsiHKAI 506 GTGCTC G[AT]GC[AT]^C
BsmAI 375 GAGAC GTCTC(1/5)
BsmFI 326 GGGAC GGGAC(10/14)
BsoBI 639 CCCGGG C^[CT]CG[GA]G
Bsp1286I 506 GTGCTC G[AGT]GC[ACT]^C
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BsrDI 330 CATTGC GCAATG(2/0)
BstBI 79 TTCGAA TT^CGAA
CfoI 75 GCGC GCG^C
DdeI 162 CTCAG C^T[ACGT]AG
DraIII 160 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 47 CCGTGG C^C[GA][CT]GG
Eco47III 74 AGCGCT AGC^GCT
FbaI 578 TGATCA T^GATCA
HaeII 74 AGCGCT [GA]GCGC^[CT]
HhaI 75 GCGC GCG^C
HinP1I 75 GCGC G^CGC
HinfI 360 GAATC G^A[ACGT]TC
MaeII 504 ACGT A^CGT
MluNI 606 TGGCCA TGG^CCA
MscI 606 TGGCCA TGG^CCA
MseI 481 TTAA T^TAA
MwoI 113 GCTGGCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 79 TTCGAA TT^CGAA
PstI 151 CTGCAG CTGCA^G
ScaI 705 AGTACT AGT^ACT
SfcI 151 CTGCAG C^T[GA][CT]AG
SfuI 79 TTCGAA TT^CGAA
SmaI 639 CCCGGG CCC^GGG
SmlI 568 CTCAAG C^T[CT][GA]AG
TfiI 360 GAATC G^A[AT]TC
Tru9I 481 TTAA T^TAA
TseI 644 GCTGC G^C[AT]GC
XcmI 38 CCAATGTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 639 CCCGGG C^CCGGG
XmaI 639 CCCGGG C^CCGGG
XmnI 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 27 GATCC GGATC(4/5)
390 GATCC
BsaJI 47 CCGTGG C^C[ACGT][ACGT]GG
639 CCCGGG
BsiYI 47 CCGTGGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCACTATTCGG
BslI 47 CCGTGGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCACTATTCGG
BsrI 309 ACTGG ACTGG(1/-1)
535 CCAGT
BstYI 26 AGATCC [GA]^GATC[CT]
207 AGATCT
EaeI 44 TGGCCG [CT]^GGCC[GA]
606 TGGCCA
Fnu4HI 122 GCGGC GC^[ACGT]GC
644 GCTGC
HaeIII 45 GGCC GG^CC
607 GGCC
HapII 68 CCGG C^CGG
640 CCGG
HpaII 68 CCGG C^CGG
640 CCGG
HphI 429 GGTGA GGTGA(8/7)
11 TCACC
ItaI 122 GCGGC GC^[ACGT]GC
644 GCTGC
MflI 26 AGATCC [GA]^GATC[CT]
207 AGATCT
MnlI 138 CCTC CCTC(7/6)
516 GAGG
MspI 68 CCGG C^CGG
640 CCGG
NlaIII 408 CATG CATG^
513 CATG
Tsp509I 657 AATT ^AATT
743 AATT
XhoII 26 AGATCC [GA]^GATC[CT]
207 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AfaI AluI BcnI BssKI
BstF5I Cac8I DpnII FokI MboI
MboII NciI NdeII RsaI Sau3AI
ScrFI TaqI TspRI TthHB8I