Restriction map for
pFN21AE5176
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BmyI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BsrI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI TspRI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AciI 219 GCGG CCGC(-3/-1)
AcsI 103 GAATTT [GA]^AATT[CT]
AlwI 189 GATCC GGATC(4/5)
ApoI 103 GAATTT [GA]^AATT[CT]
Asp700I 241 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 336 GGACC G^G[AT]CC
BanI 77 GGCACC G^G[CT][GA]CC
BbvI 156 GCTGC GCAGC(8/12)
BlpI 289 GCTGAGC GC^T[ACGT]AGC
BpmI 151 CTCCAG CTGGAG(16/14)
Bpu1102I 289 GCTGAGC GC^T[ACGT]AGC
BseRI 229 GAGGAG GAGGAG(10/8)
BsiYI 192 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 192 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 43 GAGAC GTCTC(1/5)
BsmBI 43 GAGACG CGTCTC(1/5)
BsmI 266 GCATTC GAATGC(1/-1)
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BsrFI 167 ACCGGC [GA]^CCGG[CT]
BstF5I 12 GGATG GGATG(2/0)
BstYI 188 AGATCC [GA]^GATC[CT]
CelII 289 GCTGAGC GC^T[ACGT]AGC
CfoI 25 GCGC GCG^C
Cfr10I 167 ACCGGC [GA]^CCGG[CT]
Cfr13I 336 GGACC G^G[ACGT]CC
DpnII 189 GATC ^GATC
DraII 335 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 335 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 12 GGATG GGATG(9/13)
HaeIII 170 GGCC GG^CC
HapII 168 CCGG C^CGG
HhaI 25 GCGC GCG^C
HinP1I 25 GCGC G^CGC
HincII 132 GTTGAC GT[CT]^[GA]AC
HindII 132 GTTGAC GT[CT]^[GA]AC
HinfI 84 GAGTC G^A[ACGT]TC
HpaII 168 CCGG C^CGG
HphI 260 TCACC GGTGA(8/7)
MaeII 46 ACGT A^CGT
MboI 189 GATC ^GATC
MflI 188 AGATCC [GA]^GATC[CT]
MseI 49 TTAA T^TAA
MspI 168 CCGG C^CGG
NdeII 189 GATC ^GATC
NlaIII 71 CATG CATG^
PflMI 192 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 84 GAGTC GAGTC(4/5)
PpuMI 335 AGGACCC [GA]G^G[AT]CC[CT]
PstI 275 CTGCAG CTGCA^G
Sau3AI 189 GATC ^GATC
Sau96I 336 GGACC G^G[ACGT]CC
SfaNI 13 GATGC GCATC(5/9)
SfcI 275 CTGCAG C^T[GA][CT]AG
Tru9I 49 TTAA T^TAA
TseI 156 GCTGC G^C[AT]GC
Van91I 192 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 188 AGATCC [GA]^GATC[CT]
XmnI 241 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwNI 154 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
284 CAGAGGCTG
BsrDI 314 GCAATG GCAATG(2/0)
322 GCAATG
Cac8I 74 GCAGGC GC[ACGT]^[ACGT]GC
273 GCCTGC
Fnu4HI 156 GCTGC GC^[ACGT]GC
219 GCGGC
ItaI 156 GCTGC GC^[ACGT]GC
219 GCGGC
MspA1I 154 CAGCTG C[AC]G^C[GT]G
193 CAGCTG
NlaIV 77 GGCACC GG[ACGT]^[ACGT]CC
336 GGACCC
PvuII 154 CAGCTG CAG^CTG
193 CAGCTG
SapI 214 GAAGAGC GCTCTTC(1/4)
244 GCTCTTC
Tsp45I 175 GTGAC ^GT[GC]AC
259 GTCAC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI DdeI EarI Ksp632I MaeIII
MboII MnlI MwoI Tsp509I