Restriction map for
pFN21AE4234
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AocI Aor51HI
ApaI AscI Asp718I AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BglI BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
AflII 1324 CTTAAG C^TTAAG
AhdI 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 394 GTGCAC G^TGCAC
AlwNI 660 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 394 GTGCAC G^TGCAC
AseI 1674 ATTAAT AT^TAAT
AsnI 1674 ATTAAT AT^TAAT
AspEI 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 620 CCCGGG C^[CT]CG[GA]G
BalI 1067 TGGCCA TGG^CCA
BciVI 651 GTATCC GTATCC(6/5)
BfrI 1324 CTTAAG C^TTAAG
BlpI 2410 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2410 GCTAAGC GC^T[ACGT]AGC
BsaWI 436 ACCGGA [AT]^CCGG[AT]
BsoBI 620 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1550 TGTACA T^GTACA
BspMI 324 GCAGGT ACCTGC(4/8)
BsrFI 2441 GCCGGC [GA]^CCGG[CT]
BsrGI 1550 TGTACA T^GTACA
BssSI 358 CACGAG CACGAG(-5/-1)
BstXI 560 CCAATAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2410 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2441 GCCGGC [GA]^CCGG[CT]
DraI 1306 TTTAAA TTT^AAA
DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1210 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2526 CCGTGG C^C[GA][CT]GG
EaeI 1067 TGGCCA [CT]^GGCC[GA]
Eam1105I 2299 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 802 GAATTC G^AATTC
HindIII 1798 AAGCTT A^AGCTT
MaeII 1613 ACGT A^CGT
MluNI 1067 TGGCCA TGG^CCA
MscI 1067 TGGCCA TGG^CCA
MspA1I 1142 CAGCTG C[AC]G^C[GT]G
NaeI 2441 GCCGGC GCC^GGC
NgoMIV 2441 GCCGGC G^CCGGC
NheI 142 GCTAGC G^CTAGC
PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 705 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1674 ATTAAT AT^TAAT
PvuII 1142 CAGCTG CAG^CTG
SexAI 1211 ACCTGGT A^CC[AT]GGT
SmaI 620 CCCGGG CCC^GGG
SphI 738 GCATGC GCATG^C
SspBI 1550 TGTACA T^GTACA
SspI 1162 AATATT AAT^ATT
StuI 901 AGGCCT AGG^CCT
XmaCI 620 CCCGGG C^CCGGG
XmaI 620 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 121 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
349 GAAGAATTTC
AvaII 29 GGTCC G^G[AT]CC
2530 GGACC
BclI 834 TGATCA T^GATCA
1089 TGATCA
BcnI 620 CCCGG CC^[GC]GG
621 CCGGG
BfaI 143 CTAG C^TAG
905 CTAG
BpmI 344 CTGGAG CTGGAG(16/14)
1451 CTGGAG
BsaI 1079 GAGACC GGTCTC(1/5)
1811 GAGACC
BsgI 2492 CTGCAC GTGCAG(16/14)
322 GTGCAG
BsiYI 1815 CCACCATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2184 CCGTATTAAGG
BslI 1815 CCACCATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2184 CCGTATTAAGG
FbaI 834 TGATCA T^GATCA
1089 TGATCA
MaeI 143 CTAG C^TAG
905 CTAG
NciI 620 CCCGG CC^[GC]GG
621 CCGGG
PstI 657 CTGCAG CTGCA^G
2511 CTGCAG
SmlI 1324 CTTAAG C^T[CT][GA]AG
1930 CTTGAG
Tsp45I 433 GTGAC ^GT[GC]AC
682 GTGAC
XcmI 899 CCAGGCCTAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1254 CCAGAAAATTCTTGG
XmnI 121 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
349 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AspHI BbsI BbvI BglII
BmyI BpuAI BsaJI BseRI BsiHKAI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII