Restriction map for
pFN21AE9446
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MflI MluI
MroI MslI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 451 GTCTAC GT^[AC][GT]AC
AciI 372 CCGC CCGC(-3/-1)
AlwI 699 GATCC GGATC(4/5)
ApaI 593 GGGCCC GGGCC^C
AseI 316 ATTAAT AT^TAAT
AsnI 316 ATTAAT AT^TAAT
AspI 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 345 GGCACC G^G[CT][GA]CC
BbsI 994 GTCTTC GAAGAC(2/6)
BbvCI 423 CCTCAGC CCTCAGC(-5/-2)
BcnI 44 CCGGG CC^[GC]GG
BpuAI 994 GTCTTC GAAGAC(2/6)
BseRI 225 GAGGAG GAGGAG(10/8)
BsmBI 1033 CGTCTC CGTCTC(1/5)
BstAPI 381 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1057 GCGC GCG^C
EcoT14I 1126 CCAAGG C^C[AT][AT]GG
HapII 44 CCGG C^CGG
HgaI 51 GACGC GACGC(5/10)
HhaI 1057 GCGC GCG^C
HinP1I 1057 GCGC G^CGC
HincII 298 GTCAAC GT[CT]^[GA]AC
HindII 298 GTCAAC GT[CT]^[GA]AC
HpaII 44 CCGG C^CGG
HphI 577 GGTGA GGTGA(8/7)
MaeII 410 ACGT A^CGT
MfeI 154 CAATTG C^AATTG
MspA1I 265 CAGCTG C[AC]G^C[GT]G
MspI 44 CCGG C^CGG
MunI 154 CAATTG C^AATTG
MwoI 381 GCAGGTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 44 CCGGG CC^[GC]GG
PflFI 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 316 ATTAAT AT^TAAT
PstI 1020 CTGCAG CTGCA^G
PvuII 265 CAGCTG CAG^CTG
SapI 1120 GCTCTTC GCTCTTC(1/4)
SbfI 1019 CCTGCAGG CCTGCA^GG
SexAI 353 ACCTGGT A^CC[AT]GGT
SfcI 1020 CTGCAG C^T[GA][CT]AG
Sse8387I 1019 CCTGCAGG CCTGCA^GG
StyI 1126 CCAAGG C^C[AT][AT]GG
TaqI 29 TCGA T^CGA
Tth111I 564 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 29 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 49 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
946 CAGAACCTG
BalI 269 TGGCCA TGG^CCA
1099 TGGCCA
BanII 593 GGGCCC G[GA]GC[CT]^C
1118 GGGCTC
BclI 545 TGATCA T^GATCA
938 TGATCA
BmyI 593 GGGCCC G[AGT]GC[ACT]^C
1118 GGGCTC
BsgI 583 GTGCAG GTGCAG(16/14)
768 GTGCAG
BsiYI 218 CCACATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1017 CCCCTGCAGGG
BslI 218 CCACATGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1017 CCCCTGCAGGG
BsmAI 335 GAGAC GTCTC(1/5)
1034 GTCTC
BsmFI 40 GGGAC GGGAC(10/14)
420 GTCCC
Bsp1286I 593 GGGCCC G[AGT]GC[ACT]^C
1118 GGGCTC
BspMI 950 ACCTGC ACCTGC(4/8)
381 GCAGGT
BstF5I 660 CATCC GGATG(2/0)
1029 CATCC
EaeI 269 TGGCCA [CT]^GGCC[GA]
1099 TGGCCA
EarI 1121 CTCTTC CTCTTC(1/4)
558 GAAGAG
FbaI 545 TGATCA T^GATCA
938 TGATCA
FokI 660 CATCC GGATG(9/13)
1029 CATCC
Ksp632I 1121 CTCTTC CTCTTC(1/4)
558 GAAGAG
MaeIII 88 GTGAC ^GT[ACGT]AC
259 GTGAC
MluNI 269 TGGCCA TGG^CCA
1099 TGGCCA
MscI 269 TGGCCA TGG^CCA
1099 TGGCCA
PpuMI 170 GGGTCCT [GA]G^G[AT]CC[CT]
491 GGGTCCT
SfaNI 680 GCATC GCATC(5/9)
827 GCATC
Tsp45I 88 GTGAC ^GT[GC]AC
259 GTGAC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AvaII BbvI BpmI
BsaJI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HinfI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI