Restriction map for
pFN21AE1759
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MfeI MflI MluI MroI
MslI MunI NaeI NarI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 529 CGCG CG^CG
AfaI 30 GTAC GT^AC
ApaI 784 GGGCCC GGGCC^C
Asp700I 36 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 97 CCCGAG C^[CT]CG[GA]G
BanII 784 GGGCCC G[GA]GC[CT]^C
BglI 149 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI 131 GTCTC GTCTC(1/5)
BsoBI 97 CCCGAG C^[CT]CG[GA]G
BstAPI 39 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 579 GGTGACC G^GT[ACGT]ACC
BstF5I 678 GGATG GGATG(2/0)
BstPI 579 GGTGACC G^GT[ACGT]ACC
BstUI 529 CGCG CG^CG
CfoI 530 GCGC GCG^C
DdeI 189 CTCAG C^T[ACGT]AG
DraIII 574 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 494 CCATGG C^C[GA][CT]GG
EagI 363 CGGCCG C^GGCCG
EclXI 363 CGGCCG C^GGCCG
Eco52I 363 CGGCCG C^GGCCG
EcoO65I 579 GGTGACC G^GT[ACGT]ACC
FokI 678 GGATG GGATG(9/13)
HhaI 530 GCGC GCG^C
HinP1I 530 GCGC G^CGC
HinfI 226 GATTC G^A[ACGT]TC
MboII 294 GAAGA GAAGA(8/7)
MseI 119 TTAA T^TAA
MvnI 529 CGCG CG^CG
NcoI 494 CCATGG C^CATGG
PpuMI 192 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 582 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 30 GTAC GT^AC
SexAI 575 ACCTGGT A^CC[AT]GGT
SfiI 148 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 156 AGGCCT AGG^CCT
TaqI 80 TCGA T^CGA
TfiI 226 GATTC G^A[AT]TC
Tru9I 119 TTAA T^TAA
TthHB8I 80 TCGA T^CGA
XcmI 50 CCAGGGACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 36 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 334 AAATTC [GA]^AATT[CT]
628 GAATTT
ApoI 334 AAATTC [GA]^AATT[CT]
628 GAATTT
AspHI 412 GTGCTC G[AT]GC[AT]^C
478 GTGCTC
AvaII 193 GGTCC G^G[AT]CC
320 GGACC
BalI 491 TGGCCA TGG^CCA
570 TGGCCA
BpmI 152 CTGGAG CTGGAG(16/14)
646 CTGGAG
BsiEI 261 CGACCG CG[GA][CT]^CG
363 CGGCCG
BsiHKAI 412 GTGCTC G[AT]GC[AT]^C
478 GTGCTC
BsrI 16 ACTGG ACTGG(1/-1)
317 ACTGG
DraII 192 AGGTCCT [GA]G^G[ACGT]CC[CT]
307 GGGGCCT
EcoO109I 192 AGGTCCT [GA]G^G[ACGT]CC[CT]
307 GGGGCCT
EcoT14I 162 CCAAGG C^C[AT][AT]GG
494 CCATGG
MluNI 491 TGGCCA TGG^CCA
570 TGGCCA
MscI 491 TGGCCA TGG^CCA
570 TGGCCA
NlaIII 495 CATG CATG^
612 CATG
SfcI 72 CTACAG C^T[GA][CT]AG
510 CTGTAG
SmlI 415 CTCAAG C^T[CT][GA]AG
559 CTCAAG
StyI 162 CCAAGG C^C[AT][AT]GG
494 CCATGG
Tsp509I 335 AATT ^AATT
629 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI SfaNI
TseI Tsp45I TspRI