Restriction map for
pFN21AB9721
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BpuAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DraIII DrdI EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MamI
MboI MfeI MflI MluI MseI
MunI NcoI NdeI NdeII NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfuI SmlI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 301 GTCGAC GT^[AC][GT]AC
AccIII 313 TCCGGA T^CCGGA
AfaI 411 GTAC GT^AC
AflIII 923 ACACGT A^C[GA][CT]GT
AhdI 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 721 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1000 GGGCCC GGGCC^C
Asp700I 597 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1162 GTGCTC G[AT]GC[AT]^C
BalI 728 TGGCCA TGG^CCA
BciVI 622 GTATCC GTATCC(6/5)
BfaI 1217 CTAG C^TAG
BlpI 1164 GCTCAGC GC^T[ACGT]AGC
BpmI 592 CTGGAG CTGGAG(16/14)
Bpu1102I 1164 GCTCAGC GC^T[ACGT]AGC
BsaAI 619 TACGTA [CT]AC^GT[GA]
BsaI 472 GAGACC GGTCTC(1/5)
BsaWI 313 TCCGGA [AT]^CCGG[AT]
BseAI 313 TCCGGA T^CCGGA
BsiHKAI 1162 GTGCTC G[AT]GC[AT]^C
BsmFI 157 GTCCC GGGAC(10/14)
BspEI 313 TCCGGA T^CCGGA
CelII 1164 GCTCAGC GC^T[ACGT]AGC
DraII 282 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 340 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 282 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 601 GAATTC G^AATTC
HgaI 549 GCGTC GACGC(5/10)
HincII 301 GTCGAC GT[CT]^[GA]AC
HindII 301 GTCGAC GT[CT]^[GA]AC
MaeI 1217 CTAG C^TAG
MboII 361 GAAGA GAAGA(8/7)
MluNI 728 TGGCCA TGG^CCA
MroI 313 TCCGGA T^CCGGA
MscI 728 TGGCCA TGG^CCA
NaeI 1027 GCCGGC GCC^GGC
NgoMIV 1027 GCCGGC G^CCGGC
PflMI 731 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 282 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 411 GTAC GT^AC
SalI 301 GTCGAC G^TCGAC
SgrAI 848 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 619 TACGTA TAC^GTA
StuI 1172 AGGCCT AGG^CCT
Van91I 731 CCATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 597 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 75 AAATTT [GA]^AATT[CT]
601 GAATTC
ApoI 75 AAATTT [GA]^AATT[CT]
601 GAATTC
BsgI 378 CTGCAC GTGCAG(16/14)
528 GTGCAG
BsiEI 650 CGGCCG CG[GA][CT]^CG
791 CGGCCG
BsmAI 472 GAGAC GTCTC(1/5)
70 GTCTC
BspMI 662 ACCTGC ACCTGC(4/8)
827 ACCTGC
BstF5I 869 CATCC GGATG(2/0)
33 GGATG
BstXI 252 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCAACGGGCTGG
EagI 650 CGGCCG C^GGCCG
791 CGGCCG
EclXI 650 CGGCCG C^GGCCG
791 CGGCCG
Eco52I 650 CGGCCG C^GGCCG
791 CGGCCG
FokI 869 CATCC GGATG(9/13)
33 GGATG
NotI 649 GCGGCCGC GC^GGCCGC
790 GCGGCCGC
SfaNI 546 GATGC GCATC(5/9)
868 GCATC
Tsp45I 539 GTGAC ^GT[GC]AC
1055 GTCAC
Tsp509I 76 AATT ^AATT
602 AATT
TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^
537 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DsaI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeII MaeIII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PleI SacII Sau96I ScrFI SfoI
SmaI TaqI TseI TthHB8I XmaCI
XmaI