Restriction map for
pFN21AB6269
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BciVI BclI BglI
BlnI BsaI BseAI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CpoI DraI
Eam1105I Eco47III EcoO65I EcoRV FbaI
FseI HpaI KpnI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 82 GTAGAC GT^[AC][GT]AC
AflII 1858 CTTAAG C^TTAAG
AflIII 1476 ACACGT A^C[GA][CT]GT
AgeI 1098 ACCGGT A^CCGGT
Alw44I 337 GTGCAC G^TGCAC
AocI 2042 CCTCAGG CC^T[ACGT]AGG
ApaLI 337 GTGCAC G^TGCAC
AspHI 337 GTGCAC G[AT]GC[AT]^C
AviII 1773 TGCGCA TGC^GCA
BalI 1190 TGGCCA TGG^CCA
BanII 73 GGGCTC G[GA]GC[CT]^C
BcgI 1776 GCAGAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1858 CTTAAG C^TTAAG
BsaAI 1477 CACGTA [CT]AC^GT[GA]
BsaBI 934 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsiHKAI 337 GTGCAC G[AT]GC[AT]^C
BspDI 1216 ATCGAT AT^CGAT
BspMI 98 ACCTGC ACCTGC(4/8)
BsrBI 473 GAGCGG CCGCTC(-3/-3)
BstXI 2027 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2042 CCTCAGG CC^T[ACGT]AGG
ClaI 1216 ATCGAT AT^CGAT
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 2042 CCTCAGG CC^T[ACGT]AGG
EcoRI 885 GAATTC G^AATTC
EcoT22I 326 ATGCAT ATGCA^T
FspI 1773 TGCGCA TGC^GCA
HincII 1102 GTTGAC GT[CT]^[GA]AC
HindII 1102 GTTGAC GT[CT]^[GA]AC
KspI 521 CCGCGG CCGC^GG
MaeII 1478 ACGT A^CGT
MamI 934 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1190 TGGCCA TGG^CCA
MscI 1190 TGGCCA TGG^CCA
NcoI 1455 CCATGG C^CATGG
NdeI 1111 CATATG CA^TATG
NsiI 326 ATGCAT ATGCA^T
PinAI 1098 ACCGGT A^CCGGT
PvuII 268 CAGCTG CAG^CTG
SacII 521 CCGCGG CCGC^GG
SphI 1667 GCATGC GCATG^C
TaqI 1217 TCGA T^CGA
TthHB8I 1217 TCGA T^CGA
XbaI 739 TCTAGA T^CTAGA
XcmI 2027 CCAAAGAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AlwNI 1244 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1906 CAGAAACTG
Asp700I 1018 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1534 GAACCTTTTC
BbeI 385 GGCGCC GGCGC^C
418 GGCGCC
BglII 90 AGATCT A^GATCT
1461 AGATCT
BlpI 60 GCTGAGC GC^T[ACGT]AGC
174 GCTCAGC
BmyI 73 GGGCTC G[AGT]GC[ACT]^C
337 GTGCAC
BpmI 1990 CTCCAG CTGGAG(16/14)
829 CTGGAG
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
174 GCTCAGC
BsaWI 406 ACCGGA [AT]^CCGG[AT]
1098 ACCGGT
BsmAI 750 GAGAC GTCTC(1/5)
1726 GTCTC
Bsp1286I 73 GGGCTC G[AGT]GC[ACT]^C
337 GTGCAC
CelII 60 GCTGAGC GC^T[ACGT]AGC
174 GCTCAGC
DraII 296 GGGTCCC [GA]G^G[ACGT]CC[CT]
464 GGGACCC
DraIII 340 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1315 CACTCTGTG
DrdI 1002 GACTTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1717 GACCTACAAGTC
EcoNI 120 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
856 CCTCTTCCAGG
EcoO109I 296 GGGTCCC [GA]G^G[ACGT]CC[CT]
464 GGGACCC
HaeII 385 GGCGCC [GA]GCGC^[CT]
418 GGCGCC
HgaI 496 GACGC GACGC(5/10)
547 GACGC
HindIII 1420 AAGCTT A^AGCTT
1628 AAGCTT
HphI 50 TCACC GGTGA(8/7)
1096 TCACC
KasI 385 GGCGCC G^GCGCC
418 GGCGCC
MspA1I 268 CAGCTG C[AC]G^C[GT]G
521 CCGCGG
NaeI 28 GCCGGC GCC^GGC
415 GCCGGC
NarI 385 GGCGCC GG^CGCC
418 GGCGCC
NgoMIV 28 GCCGGC G^CCGGC
415 GCCGGC
NspI 1667 GCATGC [GA]CATG^[CT]
1691 GCATGT
PpuMI 296 GGGTCCC [GA]G^G[AT]CC[CT]
464 GGGACCC
PstI 124 CTGCAG CTGCA^G
1027 CTGCAG
SfaNI 325 GATGC GCATC(5/9)
836 GCATC
SfoI 385 GGCGCC GGC^GCC
418 GGCGCC
SmlI 1602 CTTGAG C^T[CT][GA]AG
1858 CTTAAG
XmnI 1018 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1534 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BbsI BbvI BcnI BfaI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI BspHI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RcaI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII