Restriction map for
pFN21AE6756
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI HaeII HapII
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MflI MluI
MroI MslI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SpeI Sse8387I StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 1105 GGCGTC G[GA]^CG[CT]C
AflII 1273 CTTAAG C^TTAAG
AflIII 1693 ACATGT A^C[GA][CT]GT
AlwI 260 GGATC GGATC(4/5)
AlwNI 1818 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 89 GGACC G^G[AT]CC
AviII 1620 TGCGCA TGC^GCA
BalI 1562 TGGCCA TGG^CCA
BanI 2022 GGTGCC G^G[CT][GA]CC
BbsI 680 GTCTTC GAAGAC(2/6)
BbvCI 649 GCTGAGG CCTCAGC(-5/-2)
BfrI 1273 CTTAAG C^TTAAG
BpuAI 680 GTCTTC GAAGAC(2/6)
BsaAI 1316 TACGTA [CT]AC^GT[GA]
BsaHI 1105 GGCGTC G[GA]^CG[CT]C
Bsp1407I 1920 TGTACA T^GTACA
BspLU11I 1693 ACATGT A^CATGT
BsrGI 1920 TGTACA T^GTACA
BssSI 272 CTCGTG CACGAG(-5/-1)
CfoI 1621 GCGC GCG^C
Cfr13I 89 GGACC G^G[ACGT]CC
DraIII 610 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1764 CCGTGG C^C[GA][CT]GG
EcoT22I 1430 ATGCAT ATGCA^T
FspI 1620 TGCGCA TGC^GCA
HgaI 1106 GCGTC GACGC(5/10)
HhaI 1621 GCGC GCG^C
Hin1I 1105 GGCGTC G[GA]^CG[CT]C
HinP1I 1621 GCGC G^CGC
HindIII 1301 AAGCTT A^AGCTT
HphI 2005 GGTGA GGTGA(8/7)
MluNI 1562 TGGCCA TGG^CCA
MscI 1562 TGGCCA TGG^CCA
NdeI 1550 CATATG CA^TATG
NsiI 1430 ATGCAT ATGCA^T
PvuII 347 CAGCTG CAG^CTG
Sau96I 89 GGACC G^G[ACGT]CC
ScaI 1203 AGTACT AGT^ACT
SnaBI 1316 TACGTA TAC^GTA
SspBI 1920 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 28 enzymes) #
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BfaI 2155 CTAG C^TAG
2287 CTAG
BglI 723 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1459 GCCTATGCGGC
BpmI 1199 CTCCAG CTGGAG(16/14)
364 CTGGAG
BsaI 47 GAGACC GGTCTC(1/5)
2083 GGTCTC
BsaJI 326 CCCTGG C^C[ACGT][ACGT]GG
1764 CCGTGG
BsiYI 1607 CCAAATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1870 CCCGTGCAAGG
BslI 1607 CCAAATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1870 CCCGTGCAAGG
BsrDI 1983 CATTGC GCAATG(2/0)
1237 GCAATG
BstBI 284 TTCGAA TT^CGAA
422 TTCGAA
DpnII 261 GATC ^GATC
2037 GATC
DraI 27 TTTAAA TTT^AAA
708 TTTAAA
EaeI 1562 TGGCCA [CT]^GGCC[GA]
2139 CGGCCA
MaeI 2155 CTAG C^TAG
2287 CTAG
MaeII 319 ACGT A^CGT
1317 ACGT
MboI 261 GATC ^GATC
2037 GATC
MfeI 266 CAATTG C^AATTG
1544 CAATTG
MspA1I 347 CAGCTG C[AC]G^C[GT]G
1818 CAGCGG
MunI 266 CAATTG C^AATTG
1544 CAATTG
NdeII 261 GATC ^GATC
2037 GATC
NspV 284 TTCGAA TT^CGAA
422 TTCGAA
PleI 1380 GACTC GAGTC(4/5)
562 GAGTC
PstI 1852 CTGCAG CTGCA^G
1977 CTGCAG
SacI 230 GAGCTC GAGCT^C
2380 GAGCTC
Sau3AI 261 GATC ^GATC
2037 GATC
SfuI 284 TTCGAA TT^CGAA
422 TTCGAA
SmlI 1273 CTTAAG C^T[CT][GA]AG
1955 CTCAAG
SphI 1278 GCATGC GCATG^C
2250 GCATGC
SspI 1053 AATATT AAT^ATT
1595 AATATT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BmyI BsgI
BsiHKAI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII NlaIV NspI RsaI SapI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I