Restriction map for
pFN21AE1701
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DdeI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 102 ACATGT A^C[GA][CT]GT
Alw44I 264 GTGCAC G^TGCAC
AlwI 195 GGATC GGATC(4/5)
ApaLI 264 GTGCAC G^TGCAC
AvaI 206 CTCGGG C^[CT]CG[GA]G
BalI 258 TGGCCA TGG^CCA
BanII 378 GAGCTC G[GA]GC[CT]^C
BglI 440 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 366 CTGGAG CTGGAG(16/14)
BsaJI 383 CCCAGG C^C[ACGT][ACGT]GG
BsoBI 206 CTCGGG C^[CT]CG[GA]G
BspLU11I 102 ACATGT A^CATGT
BspMI 549 ACCTGC ACCTGC(4/8)
BsrBI 577 GAGCGG CCGCTC(-3/-3)
BstF5I 45 CATCC GGATG(2/0)
BstNI 384 CCAGG CC^[AT]GG
DpnII 196 GATC ^GATC
DraI 70 TTTAAA TTT^AAA
EaeI 258 TGGCCA [CT]^GGCC[GA]
EcoRII 384 CCAGG ^CC[AT]GG
EcoT22I 42 ATGCAT ATGCA^T
FokI 45 CATCC GGATG(9/13)
HaeIII 259 GGCC GG^CC
HphI 547 TCACC GGTGA(8/7)
MaeII 145 ACGT A^CGT
MboI 196 GATC ^GATC
MluNI 258 TGGCCA TGG^CCA
MscI 258 TGGCCA TGG^CCA
MslI 45 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 430 CAGCTG C[AC]G^C[GT]G
MvaI 384 CCAGG CC^[AT]GG
NdeII 196 GATC ^GATC
NsiI 42 ATGCAT ATGCA^T
PleI 520 GAGTC GAGTC(4/5)
PstI 433 CTGCAG CTGCA^G
PvuII 430 CAGCTG CAG^CTG
SacI 378 GAGCTC GAGCT^C
Sau3AI 196 GATC ^GATC
ScaI 362 AGTACT AGT^ACT
SfcI 433 CTGCAG C^T[GA][CT]AG
SphI 40 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 393 GCGG CCGC(-3/-1)
579 GCGG
BcnI 225 CCGGG CC^[GC]GG
506 CCCGG
BseRI 344 GAGGAG GAGGAG(10/8)
375 GAGGAG
BsiYI 279 CCCTCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
383 CCCAGGAGTGG
BslI 279 CCCTCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
383 CCCAGGAGTGG
HapII 225 CCGG C^CGG
507 CCGG
HpaII 225 CCGG C^CGG
507 CCGG
MseI 71 TTAA T^TAA
110 TTAA
MspI 225 CCGG C^CGG
507 CCGG
NciI 225 CCGGG CC^[GC]GG
506 CCCGG
TfiI 150 GAATC G^A[AT]TC
425 GAATC
Tru9I 71 TTAA T^TAA
110 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI AspHI BbvI BmyI
BsiHKAI Bsp1286I BsrI BssKI Cac8I
Fnu4HI HinfI ItaI MaeIII MnlI
MwoI NlaIII NspI RsaI ScrFI
SfaNI TseI Tsp45I TspRI