Restriction map for
pFN21AE2614
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI Eam1105I Eco47III EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 871 GACGTC GACGT^C
AccI 1303 GTCTAC GT^[AC][GT]AC
Alw44I 847 GTGCAC G^TGCAC
AlwNI 450 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 132 CCTTAGG CC^T[ACGT]AGG
ApaI 432 GGGCCC GGGCC^C
ApaLI 847 GTGCAC G^TGCAC
BbsI 1276 GTCTTC GAAGAC(2/6)
BciVI 162 GGATAC GTATCC(6/5)
BglI 803 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1276 GTCTTC GAAGAC(2/6)
BsaAI 1039 TACGTG [CT]AC^GT[GA]
BsiEI 790 CGGCCG CG[GA][CT]^CG
BsmAI 384 GAGAC GTCTC(1/5)
BspLU11I 1472 ACATGT A^CATGT
BsrFI 946 ACCGGC [GA]^CCGG[CT]
BssHII 681 GCGCGC G^CGCGC
BssSI 937 CACGAG CACGAG(-5/-1)
BstEII 942 GGTGACC G^GT[ACGT]ACC
BstPI 942 GGTGACC G^GT[ACGT]ACC
Bsu36I 132 CCTTAGG CC^T[ACGT]AGG
Cfr10I 946 ACCGGC [GA]^CCGG[CT]
DrdI 1485 GACGCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 790 CGGCCG C^GGCCG
EclXI 790 CGGCCG C^GGCCG
Eco52I 790 CGGCCG C^GGCCG
Eco81I 132 CCTTAGG CC^T[ACGT]AGG
EcoO65I 942 GGTGACC G^GT[ACGT]ACC
KspI 982 CCGCGG CCGC^GG
PaeR7I 1120 CTCGAG C^TCGAG
PflMI 1245 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 301 GGGACCT [GA]G^G[AT]CC[CT]
PstI 469 CTGCAG CTGCA^G
SacII 982 CCGCGG CCGC^GG
SmaI 57 CCCGGG CCC^GGG
SphI 1316 GCATGC GCATG^C
Van91I 1245 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1163 CCATCCTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1120 CTCGAG C^TCGAG
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 145 GGCGTC G[GA]^CG[CT]C
871 GACGTC
AflIII 461 ACGTGT A^C[GA][CT]GT
1472 ACATGT
AspI 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCGTGTC
BanI 520 GGCACC G^G[CT][GA]CC
1240 GGCACC
BcgI 659 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
761 GCAGCCGCTTCG
BpmI 97 CTGGAG CTGGAG(16/14)
721 CTGGAG
BsaHI 145 GGCGTC G[GA]^CG[CT]C
871 GACGTC
BseRI 28 GAGGAG GAGGAG(10/8)
100 GAGGAG
BspMI 281 ACCTGC ACCTGC(4/8)
1247 ACCTGC
BstXI 500 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1400 CCATGATCCTGG
BstYI 1130 AGATCC [GA]^GATC[CT]
1208 AGATCC
DraIII 937 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1098 CACGCTGTG
EcoT14I 428 CCTTGG C^C[AT][AT]GG
988 CCAAGG
HaeII 20 AGCGCC [GA]GCGC^[CT]
717 GGCGCT
Hin1I 145 GGCGTC G[GA]^CG[CT]C
871 GACGTC
HinfI 913 GAGTC G^A[ACGT]TC
1123 GAGTC
MflI 1130 AGATCC [GA]^GATC[CT]
1208 AGATCC
PflFI 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCGTGTC
PleI 913 GAGTC GAGTC(4/5)
1123 GAGTC
SapI 474 GCTCTTC GCTCTTC(1/4)
774 GCTCTTC
SfcI 469 CTGCAG C^T[GA][CT]AG
1515 CTACAG
SmlI 25 CTTGAG C^T[CT][GA]AG
1120 CTCGAG
StyI 428 CCTTGG C^C[AT][AT]GG
988 CCAAGG
Tth111I 856 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1461 GACCGTGTC
XhoII 1130 AGATCC [GA]^GATC[CT]
1208 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI Tsp45I TspRI
TthHB8I