Restriction map for
pFN21AE9410
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflIII AhdI Alw44I AlwI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfaI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
BstBI BstYI ClaI CpoI DpnII
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI Ksp632I KspI
MaeI MaeII MboI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I Tth111I TthHB8I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 335 GGTACC G^GTACC
AccII 96 CGCG CG^CG
AflII 361 CTTAAG C^TTAAG
AgeI 332 ACCGGT A^CCGGT
AocI 382 CCTCAGG CC^T[ACGT]AGG
Asp718I 335 GGTACC G^GTACC
BbsI 886 GTCTTC GAAGAC(2/6)
BbvCI 285 CCTCAGC CCTCAGC(-5/-2)
BfrI 361 CTTAAG C^TTAAG
BlpI 17 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 17 GCTCAGC GC^T[ACGT]AGC
BpuAI 886 GTCTTC GAAGAC(2/6)
BsaBI 732 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 399 GGTCTC GGTCTC(1/5)
BsaWI 332 ACCGGT [AT]^CCGG[AT]
BseRI 380 CTCCTC GAGGAG(10/8)
BsiWI 143 CGTACG C^GTACG
BsrDI 587 GCAATG GCAATG(2/0)
BsrFI 332 ACCGGT [GA]^CCGG[CT]
BsrI 666 ACTGG ACTGG(1/-1)
Bst1107I 841 GTATAC GTA^TAC
BstAPI 819 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 669 GGTCACC G^GT[ACGT]ACC
BstPI 669 GGTCACC G^GT[ACGT]ACC
BstUI 96 CGCG CG^CG
BstZ17I 841 GTATAC GTA^TAC
Bsu36I 382 CCTCAGG CC^T[ACGT]AGG
CelII 17 GCTCAGC GC^T[ACGT]AGC
Cfr10I 332 ACCGGT [GA]^CCGG[CT]
Eco81I 382 CCTCAGG CC^T[ACGT]AGG
EcoNI 875 CCTATCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 669 GGTCACC G^GT[ACGT]ACC
EcoT14I 674 CCTTGG C^C[AT][AT]GG
FauI 654 GCGGG CCCGC(4/6)
HaeII 102 GGCGCT [GA]GCGC^[CT]
HincII 367 GTCAAC GT[CT]^[GA]AC
HindII 367 GTCAAC GT[CT]^[GA]AC
HindIII 646 AAGCTT A^AGCTT
KpnI 335 GGTACC GGTAC^C
MamI 732 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 362 TTAA T^TAA
MvnI 96 CGCG CG^CG
PinAI 332 ACCGGT A^CCGGT
PstI 759 CTGCAG CTGCA^G
PvuII 44 CAGCTG CAG^CTG
SacI 870 GAGCTC GAGCT^C
SexAI 478 ACCAGGT A^CC[AT]GGT
SfcI 759 CTGCAG C^T[GA][CT]AG
SfiI 306 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 441 CCCGGG CCC^GGG
SmlI 361 CTTAAG C^T[CT][GA]AG
StyI 674 CCTTGG C^C[AT][AT]GG
TfiI 747 GAATC G^A[AT]TC
Tru9I 362 TTAA T^TAA
XcmI 26 CCAGCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 441 CCCGGG C^CCGGG
XmaI 441 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 151 GTCTAC GT^[AC][GT]AC
841 GTATAC
AfaI 144 GTAC GT^AC
336 GTAC
AlwNI 661 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
745 CAGAATCTG
AspHI 826 GTGCTC G[AT]GC[AT]^C
870 GAGCTC
AvaI 402 CTCGGG C^[CT]CG[GA]G
441 CCCGGG
BalI 38 TGGCCA TGG^CCA
719 TGGCCA
BanI 335 GGTACC G^G[CT][GA]CC
482 GGTGCC
BsgI 817 CTGCAC GTGCAG(16/14)
773 GTGCAG
BsiHKAI 826 GTGCTC G[AT]GC[AT]^C
870 GAGCTC
BsoBI 402 CTCGGG C^[CT]CG[GA]G
441 CCCGGG
BstF5I 302 GGATG GGATG(2/0)
792 GGATG
BstXI 26 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
41 CCACAGCTGTGG
CfoI 103 GCGC GCG^C
270 GCGC
DraIII 477 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
820 CACAGTGTG
DsaI 320 CCGTGG C^C[GA][CT]GG
836 CCGTGG
FokI 302 GGATG GGATG(9/13)
792 GGATG
HhaI 103 GCGC GCG^C
270 GCGC
HinP1I 103 GCGC G^CGC
270 GCGC
MaeIII 670 GTCAC ^GT[ACGT]AC
796 GTCAC
MboII 740 TCTTC GAAGA(8/7)
887 TCTTC
MluNI 38 TGGCCA TGG^CCA
719 TGGCCA
MscI 38 TGGCCA TGG^CCA
719 TGGCCA
MspA1I 44 CAGCTG C[AC]G^C[GT]G
652 CAGCGG
PflMI 660 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
744 CCAGAATCTGG
RsaI 144 GTAC GT^AC
336 GTAC
Tsp45I 670 GTCAC ^GT[GC]AC
796 GTCAC
Van91I 660 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
744 CCAGAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI ApaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspMI BssKI BstNI Cac8I Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MnlI MslI MspI
MvaI MwoI NciI NlaIV PleI
Sau96I ScrFI SfaNI TseI TspRI