Restriction map for
pFN21AE1741
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BmyI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 180 GAATTT [GA]^AATT[CT]
AfaI 51 GTAC GT^AC
AlwNI 684 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 180 GAATTT [GA]^AATT[CT]
AspI 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 400 GGATAC GTATCC(6/5)
BcnI 71 CCGGG CC^[GC]GG
BfaI 232 CTAG C^TAG
BlpI 650 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 650 GCTTAGC GC^T[ACGT]AGC
BsaI 555 GGTCTC GGTCTC(1/5)
BseRI 448 CTCCTC GAGGAG(10/8)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BstEII 226 GGTCACC G^GT[ACGT]ACC
BstPI 226 GGTCACC G^GT[ACGT]ACC
BstYI 412 GGATCT [GA]^GATC[CT]
CelII 650 GCTTAGC GC^T[ACGT]AGC
CfoI 33 GCGC GCG^C
DraIII 344 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoO65I 226 GGTCACC G^GT[ACGT]ACC
EcoT14I 638 CCATGG C^C[AT][AT]GG
HapII 71 CCGG C^CGG
HgaI 88 GACGC GACGC(5/10)
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
HpaII 71 CCGG C^CGG
MaeI 232 CTAG C^TAG
MflI 412 GGATCT [GA]^GATC[CT]
MspI 71 CCGG C^CGG
NciI 71 CCGGG CC^[GC]GG
NcoI 638 CCATGG C^CATGG
NotI 7 GCGGCCGC GC^GGCCGC
NruI 38 TCGCGA TCG^CGA
PflFI 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 457 GACTC GAGTC(4/5)
PstI 364 CTGCAG CTGCA^G
PvuII 241 CAGCTG CAG^CTG
RsaI 51 GTAC GT^AC
SapI 235 GAAGAGC GCTCTTC(1/4)
SfcI 364 CTGCAG C^T[GA][CT]AG
StyI 638 CCATGG C^C[AT][AT]GG
TfiI 42 GATTC G^A[AT]TC
Tsp509I 181 AATT ^AATT
Tth111I 208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 412 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsmAI 566 GAGAC GTCTC(1/5)
556 GTCTC
BspMI 53 ACCTGC ACCTGC(4/8)
366 GCAGGT
DsaI 408 CCGTGG C^C[GA][CT]GG
638 CCATGG
EarI 235 GAAGAG CTCTTC(1/4)
609 GAAGAG
FauI 19 CCCGC CCCGC(4/6)
81 GCGGG
HinfI 42 GATTC G^A[ACGT]TC
457 GACTC
HphI 113 TCACC GGTGA(8/7)
228 TCACC
Ksp632I 235 GAAGAG CTCTTC(1/4)
609 GAAGAG
MaeIII 206 GTGAC ^GT[ACGT]AC
227 GTCAC
MspA1I 5 CAGCGG C[AC]G^C[GT]G
241 CAGCTG
NlaIII 136 CATG CATG^
639 CATG
TaqI 62 TCGA T^CGA
431 TCGA
Tsp45I 206 GTGAC ^GT[GC]AC
227 GTCAC
TthHB8I 62 TCGA T^CGA
431 TCGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MvaI MvnI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI