Restriction map for
pFN21AE5533
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MflI MluI
MroI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 124 GACGCC G[GA]^CG[CT]C
AhdI 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 664 CCTGAGG CC^T[ACGT]AGG
Asp700I 553 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1230 TGGCCA TGG^CCA
BsaHI 124 GACGCC G[GA]^CG[CT]C
BsaWI 699 ACCGGA [AT]^CCGG[AT]
BsmAI 973 GAGAC GTCTC(1/5)
BsmI 450 GAATGC GAATGC(1/-1)
BspHI 405 TCATGA T^CATGA
BsrBI 565 CCGCTC CCGCTC(-3/-3)
BsrDI 626 CATTGC GCAATG(2/0)
Bsu36I 664 CCTGAGG CC^T[ACGT]AGG
DsaI 1278 CCATGG C^C[GA][CT]GG
EaeI 1230 TGGCCA [CT]^GGCC[GA]
Eam1105I 892 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 568 CTCTTC CTCTTC(1/4)
Eco81I 664 CCTGAGG CC^T[ACGT]AGG
HaeII 88 AGCGCC [GA]GCGC^[CT]
HapII 700 CCGG C^CGG
HgaI 124 GACGC GACGC(5/10)
Hin1I 124 GACGCC G[GA]^CG[CT]C
HpaII 700 CCGG C^CGG
Ksp632I 568 CTCTTC CTCTTC(1/4)
MfeI 944 CAATTG C^AATTG
MluNI 1230 TGGCCA TGG^CCA
MscI 1230 TGGCCA TGG^CCA
MslI 863 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 700 CCGG C^CGG
MunI 944 CAATTG C^AATTG
NcoI 1278 CCATGG C^CATGG
PflMI 341 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 115 GAGTC GAGTC(4/5)
PpuMI 1069 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 828 CTGCAG CTGCA^G
RcaI 405 TCATGA T^CATGA
SapI 567 GCTCTTC GCTCTTC(1/4)
StuI 365 AGGCCT AGG^CCT
TaqI 101 TCGA T^CGA
TthHB8I 101 TCGA T^CGA
Van91I 341 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1238 CCAAAGCTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 553 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcsI 262 GAATTT [GA]^AATT[CT]
328 AAATTT
AfaI 537 GTAC GT^AC
1155 GTAC
AlwI 1121 GATCC GGATC(4/5)
702 GGATC
ApaI 466 GGGCCC GGGCC^C
1012 GGGCCC
ApoI 262 GAATTT [GA]^AATT[CT]
328 AAATTT
AspI 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1132 GACACTGTC
AvaII 310 GGACC G^G[AT]CC
1070 GGTCC
BanI 373 GGTGCC G^G[CT][GA]CC
1027 GGCACC
BseRI 692 GAGGAG GAGGAG(10/8)
1048 GAGGAG
BsmFI 476 GGGAC GGGAC(10/14)
117 GTCCC
BspMI 521 ACCTGC ACCTGC(4/8)
826 ACCTGC
CfoI 89 GCGC GCG^C
96 GCGC
DraII 465 GGGGCCC [GA]G^G[ACGT]CC[CT]
1069 GGGTCCT
EcoO109I 465 GGGGCCC [GA]G^G[ACGT]CC[CT]
1069 GGGTCCT
EcoT14I 1227 CCTTGG C^C[AT][AT]GG
1278 CCATGG
FauI 128 CCCGC CCCGC(4/6)
139 CCCGC
HhaI 89 GCGC GCG^C
96 GCGC
HinP1I 89 GCGC G^CGC
96 GCGC
MspA1I 133 CAGCTG C[AC]G^C[GT]G
1219 CAGCTG
PflFI 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1132 GACACTGTC
PvuII 133 CAGCTG CAG^CTG
1219 CAGCTG
RsaI 537 GTAC GT^AC
1155 GTAC
SacI 13 GAGCTC GAGCT^C
634 GAGCTC
SmlI 493 CTTGAG C^T[CT][GA]AG
574 CTTGAG
SspI 434 AATATT AAT^ATT
1006 AATATT
StyI 1227 CCTTGG C^C[AT][AT]GG
1278 CCATGG
TfiI 583 GAATC G^A[AT]TC
926 GAATC
Tth111I 478 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1132 GACACTGTC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI