Restriction map for
pFN21AE5720
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflIII AgeI AhdI AlwNI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 460 GTCTAC GT^[AC][GT]AC
AclI 1423 AACGTT AA^CGTT
AfaI 1271 GTAC GT^AC
Alw44I 45 GTGCAC G^TGCAC
Aor51HI 276 AGCGCT AGC^GCT
ApaLI 45 GTGCAC G^TGCAC
AspHI 45 GTGCAC G[AT]GC[AT]^C
AvaII 99 GGACC G^G[AT]CC
BalI 759 TGGCCA TGG^CCA
BamHI 1122 GGATCC G^GATCC
BanII 232 GAGCCC G[GA]GC[CT]^C
BbsI 4 GAAGAC GAAGAC(2/6)
BciVI 357 GTATCC GTATCC(6/5)
BclI 1218 TGATCA T^GATCA
BcnI 1094 CCGGG CC^[GC]GG
BglII 942 AGATCT A^GATCT
BpuAI 4 GAAGAC GAAGAC(2/6)
BsiHKAI 45 GTGCAC G[AT]GC[AT]^C
BsmAI 210 GTCTC GTCTC(1/5)
BstXI 80 CCAGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 277 GCGC GCG^C
DraI 18 TTTAAA TTT^AAA
DraII 98 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 201 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1130 CCATGG C^C[GA][CT]GG
EaeI 759 TGGCCA [CT]^GGCC[GA]
Eco47III 276 AGCGCT AGC^GCT
EcoNI 1781 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 98 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 75 GAATTC G^AATTC
EcoRV 1791 GATATC GAT^ATC
FauI 1014 CCCGC CCCGC(4/6)
FbaI 1218 TGATCA T^GATCA
HaeII 276 AGCGCT [GA]GCGC^[CT]
HhaI 277 GCGC GCG^C
HinP1I 277 GCGC G^CGC
HincII 1354 GTTGAC GT[CT]^[GA]AC
HindII 1354 GTTGAC GT[CT]^[GA]AC
MluNI 759 TGGCCA TGG^CCA
MscI 759 TGGCCA TGG^CCA
MslI 1228 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1094 CCGGG CC^[GC]GG
NcoI 1130 CCATGG C^CATGG
NlaIV 1122 GGATCC GG[ACGT]^[ACGT]CC
NspI 1775 ACATGC [GA]CATG^[CT]
PflMI 1824 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 98 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1423 AACGTT AA^CGTT
RsaI 1271 GTAC GT^AC
SspI 1645 AATATT AAT^ATT
SwaI 17 ATTTAAAT ATTT^AAAT
Van91I 1824 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 503 CCACTTACGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 1015 CCGC CCGC(-3/-1)
1152 CCGC
AcsI 75 GAATTC [GA]^AATT[CT]
714 AAATTT
AflII 1266 CTTAAG C^TTAAG
1519 CTTAAG
ApoI 75 GAATTC [GA]^AATT[CT]
714 AAATTT
BfrI 1266 CTTAAG C^TTAAG
1519 CTTAAG
BmyI 45 GTGCAC G[AGT]GC[ACT]^C
232 GAGCCC
BseRI 1210 GAGGAG GAGGAG(10/8)
1346 GAGGAG
Bsp1286I 45 GTGCAC G[AGT]GC[ACT]^C
232 GAGCCC
BstAPI 1309 GCAAGTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1770 GCACAACATGC
BstYI 942 AGATCT [GA]^GATC[CT]
1122 GGATCC
Cfr13I 99 GGACC G^G[ACGT]CC
1441 GGCCC
HapII 996 CCGG C^CGG
1094 CCGG
HpaII 996 CCGG C^CGG
1094 CCGG
HphI 133 GGTGA GGTGA(8/7)
1091 TCACC
MflI 942 AGATCT [GA]^GATC[CT]
1122 GGATCC
MspA1I 352 CAGCTG C[AC]G^C[GT]G
1304 CAGCTG
MspI 996 CCGG C^CGG
1094 CCGG
MwoI 1309 GCAAGTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1770 GCACAACATGC
PvuII 352 CAGCTG CAG^CTG
1304 CAGCTG
Sau96I 99 GGACC G^G[ACGT]CC
1441 GGCCC
SmlI 1266 CTTAAG C^T[CT][GA]AG
1519 CTTAAG
TaqI 817 TCGA T^CGA
908 TCGA
Tsp45I 1028 GTGAC ^GT[GC]AC
1799 GTCAC
TthHB8I 817 TCGA T^CGA
908 TCGA
XhoII 942 AGATCT [GA]^GATC[CT]
1122 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AlwI BbvI BfaI BpmI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
PleI Sau3AI ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI