Restriction map for
pFN21AE9415
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AvaI BamHI
BanII BbvCI BcgI BciVI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
Bst1107I BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FokI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp45I
Tsp509I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 925 GTCTAC GT^[AC][GT]AC
AhdI 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 553 GTGCAC G^TGCAC
AlwI 1005 GATCC GGATC(4/5)
ApaLI 553 GTGCAC G^TGCAC
AspEI 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 588 CCTAGG C^CTAGG
BbrPI 676 CACGTG CAC^GTG
BbsI 709 GTCTTC GAAGAC(2/6)
BclI 968 TGATCA T^GATCA
BlnI 588 CCTAGG C^CTAGG
BpuAI 709 GTCTTC GAAGAC(2/6)
BsgI 724 CTGCAC GTGCAG(16/14)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BsrBI 59 GAGCGG CCGCTC(-3/-3)
BsrFI 136 GCCGGC [GA]^CCGG[CT]
BssSI 442 CTCGTG CACGAG(-5/-1)
BstAPI 555 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 482 CCATTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1004 AGATCC [GA]^GATC[CT]
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
DraIII 382 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 916 GACCCGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 220 CCGTGG C^C[GA][CT]GG
EagI 50 CGGCCG C^GGCCG
Eam1105I 414 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
EcoT14I 588 CCTAGG C^C[AT][AT]GG
FbaI 968 TGATCA T^GATCA
HgaI 292 GCGTC GACGC(5/10)
MflI 1004 AGATCC [GA]^GATC[CT]
NaeI 136 GCCGGC GCC^GGC
NgoMIV 136 GCCGGC G^CCGGC
PflFI 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 676 CACGTG CAC^GTG
PmlI 676 CACGTG CAC^GTG
ScaI 395 AGTACT AGT^ACT
StuI 591 AGGCCT AGG^CCT
StyI 588 CCTAGG C^C[AT][AT]GG
TfiI 216 GAATC G^A[AT]TC
Tth111I 579 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1004 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 229 GTGCTC G[AT]GC[AT]^C
553 GTGCAC
AviII 308 TGCGCA TGC^GCA
737 TGCGCA
BalI 341 TGGCCA TGG^CCA
479 TGGCCA
BcnI 193 CCGGG CC^[GC]GG
918 CCCGG
BfaI 130 CTAG C^TAG
589 CTAG
BpmI 160 CTGGAG CTGGAG(16/14)
260 CTGGAG
BsaAI 676 CACGTG [CT]AC^GT[GA]
733 TACGTG
BsaI 155 GGTCTC GGTCTC(1/5)
1070 GGTCTC
BsiHKAI 229 GTGCTC G[AT]GC[AT]^C
553 GTGCAC
DraII 108 GGGACCC [GA]G^G[ACGT]CC[CT]
914 AGGACCC
EcoO109I 108 GGGACCC [GA]G^G[ACGT]CC[CT]
914 AGGACCC
FspI 308 TGCGCA TGC^GCA
737 TGCGCA
HphI 213 GGTGA GGTGA(8/7)
521 TCACC
MaeI 130 CTAG C^TAG
589 CTAG
MboII 701 TCTTC GAAGA(8/7)
710 TCTTC
MluNI 341 TGGCCA TGG^CCA
479 TGGCCA
MscI 341 TGGCCA TGG^CCA
479 TGGCCA
NciI 193 CCGGG CC^[GC]GG
918 CCCGG
PflMI 33 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
393 CCAGTACTTGG
PpuMI 108 GGGACCC [GA]G^G[AT]CC[CT]
914 AGGACCC
PstI 645 CTGCAG CTGCA^G
781 CTGCAG
PvuII 175 CAGCTG CAG^CTG
870 CAGCTG
SfaNI 716 GCATC GCATC(5/9)
740 GCATC
SfcI 645 CTGCAG C^T[GA][CT]AG
781 CTGCAG
Van91I 33 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
393 CCAGTACTTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbeI BbvI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfoI TseI
TspRI XcmI