Restriction map for
pFN21AE4060
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I FbaI FokI FseI
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI MaeI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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ApaI 9 GGGCCC GGGCC^C
AspHI 256 GAGCTC G[AT]GC[AT]^C
AvaII 225 GGACC G^G[AT]CC
AviII 308 TGCGCA TGC^GCA
BamHI 240 GGATCC G^GATCC
BpmI 157 CTGGAG CTGGAG(16/14)
BsaBI 235 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 256 GAGCTC G[AT]GC[AT]^C
BsmBI 955 CGTCTC CGTCTC(1/5)
Bsp1407I 701 TGTACA T^GTACA
BsrDI 781 GCAATG GCAATG(2/0)
BsrGI 701 TGTACA T^GTACA
BssHII 92 GCGCGC G^CGCGC
DraII 204 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 352 CTCTTC CTCTTC(1/4)
EcoNI 183 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 204 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 762 ATGCAT ATGCA^T
FauI 397 GCGGG CCCGC(4/6)
FspI 308 TGCGCA TGC^GCA
HaeII 313 AGCGCC [GA]GCGC^[CT]
Ksp632I 352 CTCTTC CTCTTC(1/4)
KspI 395 CCGCGG CCGC^GG
MaeII 954 ACGT A^CGT
MaeIII 839 GTGAC ^GT[ACGT]AC
MamI 235 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NlaIII 494 CATG CATG^
NsiI 762 ATGCAT ATGCA^T
PstI 187 CTGCAG CTGCA^G
SacI 256 GAGCTC GAGCT^C
SacII 395 CCGCGG CCGC^GG
SbfI 186 CCTGCAGG CCTGCA^GG
SfaNI 778 GATGC GCATC(5/9)
SfcI 187 CTGCAG C^T[GA][CT]AG
Sse8387I 186 CCTGCAGG CCTGCA^GG
SspBI 701 TGTACA T^GTACA
SspI 982 AATATT AAT^ATT
TfiI 468 GATTC G^A[AT]TC
Tsp45I 839 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 478 GAATTT [GA]^AATT[CT]
993 AAATTC
AfaI 702 GTAC GT^AC
968 GTAC
AhdI 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
294 GACAGGGTGTC
AlwNI 151 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
845 CAGTGCCTG
ApoI 478 GAATTT [GA]^AATT[CT]
993 AAATTC
AspEI 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
294 GACAGGGTGTC
BanII 9 GGGCCC G[GA]GC[CT]^C
256 GAGCTC
BbsI 124 GTCTTC GAAGAC(2/6)
892 GTCTTC
BglI 42 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
54 GCCGCTGGGGC
BmyI 9 GGGCCC G[AGT]GC[ACT]^C
256 GAGCTC
BpuAI 124 GTCTTC GAAGAC(2/6)
892 GTCTTC
BsmAI 434 GTCTC GTCTC(1/5)
956 GTCTC
BsmI 452 GAATGC GAATGC(1/-1)
618 GAATGC
Bsp1286I 9 GGGCCC G[AGT]GC[ACT]^C
256 GAGCTC
BsrI 965 CCAGT ACTGG(1/-1)
971 CCAGT
BstNI 87 CCTGG CC^[AT]GG
748 CCTGG
BstYI 234 GGATCT [GA]^GATC[CT]
240 GGATCC
Cac8I 81 GCTGGC GC[ACGT]^[ACGT]GC
92 GCGCGC
DpnII 235 GATC ^GATC
241 GATC
DsaI 46 CCGTGG C^C[GA][CT]GG
395 CCGCGG
Eam1105I 133 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
294 GACAGGGTGTC
EcoRII 87 CCTGG ^CC[AT]GG
748 CCTGG
MboI 235 GATC ^GATC
241 GATC
MflI 234 GGATCT [GA]^GATC[CT]
240 GGATCC
MslI 755 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
765 CATTGATGTG
MvaI 87 CCTGG CC^[AT]GG
748 CCTGG
NdeII 235 GATC ^GATC
241 GATC
PleI 414 GAGTC GAGTC(4/5)
807 GAGTC
RsaI 702 GTAC GT^AC
968 GTAC
Sau3AI 235 GATC ^GATC
241 GATC
SmlI 730 CTTGAG C^T[CT][GA]AG
958 CTCAAG
TaqI 795 TCGA T^CGA
810 TCGA
TthHB8I 795 TCGA T^CGA
810 TCGA
XhoII 234 GGATCT [GA]^GATC[CT]
240 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BsaJI BsiYI BslI BsmFI
BspMI BssKI BstAPI BstUI CfoI
Cfr13I DdeI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboII MnlI MseI MspA1I MspI
MvnI MwoI NciI NlaIV Sau96I
ScrFI Tru9I TseI Tsp509I TspRI