##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MamI        MluI        MluNI       MroI        
MscI        MspA1I      MvaI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI           44  GAATTC               [GA]^AATT[CT]
AfaI          304  GTAC                 GT^AC
AlwI          682  GATCC                GGATC(4/5)
ApoI           44  GAATTC               [GA]^AATT[CT]
BbsI          187  GAAGAC               GAAGAC(2/6)
BbvI          619  GCTGC                GCAGC(8/12)
BcnI          780  CCCGG                CC^[GC]GG
BfaI          643  CTAG                 C^TAG
BglII         648  AGATCT               A^GATCT
BpuAI         187  GAAGAC               GAAGAC(2/6)
BsaJI         289  CCTTGG               C^C[ACGT][ACGT]GG
BseRI         652  CTCCTC               GAGGAG(10/8)
BsgI          337  CTGCAC               GTGCAG(16/14)
BsmAI         180  GAGAC                GTCTC(1/5)
BsrI          489  CCAGT                ACTGG(1/-1)
BssKI         780  CCCGG                ^CC[ACGT]GG
BstXI         226  CCACTGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         648  AGATCT               [GA]^GATC[CT]
Cfr13I        497  GGGCC                G^G[ACGT]CC
DraI          402  TTTAAA               TTT^AAA
EarI          284  CTCTTC               CTCTTC(1/4)
EcoRI          44  GAATTC               G^AATTC
EcoT14I       289  CCTTGG               C^C[AT][AT]GG
Fnu4HI        619  GCTGC                GC^[ACGT]GC
HaeIII        498  GGCC                 GG^CC
HapII         781  CCGG                 C^CGG
HpaII         781  CCGG                 C^CGG
ItaI          619  GCTGC                GC^[ACGT]GC
Ksp632I       284  CTCTTC               CTCTTC(1/4)
MaeI          643  CTAG                 C^TAG
MaeII         136  ACGT                 A^CGT
MaeIII        142  GTAAC                ^GT[ACGT]AC
MflI          648  AGATCT               [GA]^GATC[CT]
MslI          227  CACTGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          781  CCGG                 C^CGG
MwoI          358  GCAATTGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          780  CCCGG                CC^[GC]GG
RsaI          304  GTAC                 GT^AC
SapI          283  GCTCTTC              GCTCTTC(1/4)
Sau96I        497  GGGCC                G^G[ACGT]CC
ScrFI         780  CCCGG                CC^[ACGT]GG
StyI          289  CCTTGG               C^C[AT][AT]GG
TaqI           24  TCGA                 T^CGA
TseI          619  GCTGC                G^C[AT]GC
TthHB8I        24  TCGA                 T^CGA
XhoII         648  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwNI         598  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              754  CAGTGGCTG           
HinfI         705  GAATC                G^A[ACGT]TC
              717  GATTC               
MfeI          245  CAATTG               C^AATTG
              359  CAATTG              
MnlI          654  CCTC                 CCTC(7/6)
              748  CCTC                
MseI          311  TTAA                 T^TAA
              403  TTAA                
MunI          245  CAATTG               C^AATTG
              359  CAATTG              
TfiI          705  GAATC                G^A[AT]TC
              717  GATTC               
Tru9I         311  TTAA                 T^TAA
              403  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BsiYI       BslI        BstF5I      DdeI        
DpnII       FokI        MboI        MboII       NdeII       
NlaIII      PstI        Sau3AI      SfaNI       SfcI        
Tsp509I     TspRI