Restriction map for
pFN21AE5593
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
AhdI AlwNI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI Eam1105I
EcoRI EcoRV EcoT14I FbaI FseI
HindIII KpnI MamI MfeI MflI
MluI MunI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tth111I
Van91I XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 848 GTCTAC GT^[AC][GT]AC
AccIII 975 TCCGGA T^CCGGA
AfaI 70 GTAC GT^AC
AflII 720 CTTAAG C^TTAAG
AflIII 752 ACGTGT A^C[GA][CT]GT
Alw44I 620 GTGCAC G^TGCAC
AocI 705 CCTCAGG CC^T[ACGT]AGG
Aor51HI 63 AGCGCT AGC^GCT
ApaI 21 GGGCCC GGGCC^C
ApaLI 620 GTGCAC G^TGCAC
AviII 278 TGCGCA TGC^GCA
BalI 650 TGGCCA TGG^CCA
BfrI 720 CTTAAG C^TTAAG
BglI 604 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 793 CTGGAG CTGGAG(16/14)
BsaI 84 GGTCTC GGTCTC(1/5)
BseAI 975 TCCGGA T^CCGGA
BsgI 943 GTGCAG GTGCAG(16/14)
BsiEI 340 CGGCCG CG[GA][CT]^CG
BsmBI 504 CGTCTC CGTCTC(1/5)
BspEI 975 TCCGGA T^CCGGA
BsrBI 565 GAGCGG CCGCTC(-3/-3)
BsrDI 494 GCAATG GCAATG(2/0)
BstEII 915 GGTGACC G^GT[ACGT]ACC
BstPI 915 GGTGACC G^GT[ACGT]ACC
Bsu36I 705 CCTCAGG CC^T[ACGT]AGG
DraIII 544 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 747 GACCTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 340 CGGCCG C^GGCCG
EarI 286 CTCTTC CTCTTC(1/4)
EclXI 340 CGGCCG C^GGCCG
Eco47III 63 AGCGCT AGC^GCT
Eco52I 340 CGGCCG C^GGCCG
Eco81I 705 CCTCAGG CC^T[ACGT]AGG
EcoNI 387 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 915 GGTGACC G^GT[ACGT]ACC
EcoT22I 1153 ATGCAT ATGCA^T
FspI 278 TGCGCA TGC^GCA
HincII 232 GTTAAC GT[CT]^[GA]AC
HindII 232 GTTAAC GT[CT]^[GA]AC
HpaI 232 GTTAAC GTT^AAC
Ksp632I 286 CTCTTC CTCTTC(1/4)
KspI 676 CCGCGG CCGC^GG
MluNI 650 TGGCCA TGG^CCA
MroI 975 TCCGGA T^CCGGA
MscI 650 TGGCCA TGG^CCA
MslI 591 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1153 ATGCAT ATGCA^T
PvuII 304 CAGCTG CAG^CTG
RsaI 70 GTAC GT^AC
SacII 676 CCGCGG CCGC^GG
SbfI 1172 CCTGCAGG CCTGCA^GG
SmaI 17 CCCGGG CCC^GGG
Sse8387I 1172 CCTGCAGG CCTGCA^GG
Tsp509I 730 AATT ^AATT
XbaI 777 TCTAGA T^CTAGA
XcmI 515 CCAGTCCCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 17 CCCGGG C^CCGGG
XmaI 17 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 534 GATCC GGATC(4/5)
1179 GGATC
AvaI 17 CCCGGG C^[CT]CG[GA]G
486 CTCGGG
BanII 21 GGGCCC G[GA]GC[CT]^C
631 GGGCTC
BbsI 93 GTCTTC GAAGAC(2/6)
1069 GTCTTC
BbvCI 372 CCTCAGC CCTCAGC(-5/-2)
444 CCTCAGC
BfaI 61 CTAG C^TAG
778 CTAG
BpuAI 93 GTCTTC GAAGAC(2/6)
1069 GTCTTC
BsaAI 274 TACGTG [CT]AC^GT[GA]
751 TACGTG
BsaWI 575 ACCGGA [AT]^CCGG[AT]
975 TCCGGA
BsmAI 85 GTCTC GTCTC(1/5)
505 GTCTC
BsoBI 17 CCCGGG C^[CT]CG[GA]G
486 CTCGGG
BspMI 263 ACCTGC ACCTGC(4/8)
671 ACCTGC
BssHII 214 GCGCGC G^CGCGC
399 GCGCGC
DpnII 534 GATC ^GATC
1180 GATC
DsaI 257 CCACGG C^C[GA][CT]GG
676 CCGCGG
HgaI 42 GCGTC GACGC(5/10)
503 GCGTC
HinfI 990 GAATC G^A[ACGT]TC
1038 GATTC
MaeI 61 CTAG C^TAG
778 CTAG
MaeII 275 ACGT A^CGT
752 ACGT
MaeIII 916 GTGAC ^GT[ACGT]AC
1031 GTGAC
MboI 534 GATC ^GATC
1180 GATC
NaeI 322 GCCGGC GCC^GGC
338 GCCGGC
NdeII 534 GATC ^GATC
1180 GATC
NgoMIV 322 GCCGGC G^CCGGC
338 GCCGGC
PpuMI 812 AGGTCCT [GA]G^G[AT]CC[CT]
995 GGGTCCT
PshAI 37 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
466 GACCTCAGTC
PstI 625 CTGCAG CTGCA^G
1173 CTGCAG
Sau3AI 534 GATC ^GATC
1180 GATC
SfcI 625 CTGCAG C^T[GA][CT]AG
1173 CTGCAG
TfiI 990 GAATC G^A[AT]TC
1038 GATTC
Tsp45I 916 GTGAC ^GT[GC]AC
1031 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI SfoI SmlI TaqI
Tru9I TseI TspRI TthHB8I