Restriction map for
pFN21AE6457
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI HphI KasI KspI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 881 GGTACC G^GTACC
AflIII 1179 ACATGT A^C[GA][CT]GT
AhdI 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 477 ATTAAT AT^TAAT
AsnI 477 ATTAAT AT^TAAT
Asp718I 881 GGTACC G^GTACC
AspEI 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1268 GAGCAC G[AT]GC[AT]^C
BanI 881 GGTACC G^G[CT][GA]CC
BbsI 190 GAAGAC GAAGAC(2/6)
BcgI 126 GCAAGGTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 17 AGATCT A^GATCT
BpuAI 190 GAAGAC GAAGAC(2/6)
BsaBI 18 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 7 ACCGGA [AT]^CCGG[AT]
BsgI 417 CTGCAC GTGCAG(16/14)
BsiHKAI 1268 GAGCAC G[AT]GC[AT]^C
BsmFI 1013 GTCCC GGGAC(10/14)
BsmI 231 GAATGC GAATGC(1/-1)
BspDI 865 ATCGAT AT^CGAT
BspLU11I 1179 ACATGT A^CATGT
BsrDI 435 GCAATG GCAATG(2/0)
BstAPI 410 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 865 ATCGAT AT^CGAT
DraI 562 TTTAAA TTT^AAA
DsaI 962 CCATGG C^C[GA][CT]GG
EaeI 794 CGGCCA [CT]^GGCC[GA]
Eam1105I 289 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HapII 8 CCGG C^CGG
HincII 371 GTCAAC GT[CT]^[GA]AC
HindII 371 GTCAAC GT[CT]^[GA]AC
HindIII 1219 AAGCTT A^AGCTT
HpaII 8 CCGG C^CGG
KpnI 881 GGTACC GGTAC^C
MamI 18 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 8 CCGG C^CGG
NcoI 962 CCATGG C^CATGG
PflMI 1304 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 477 ATTAAT AT^TAAT
Van91I 1304 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 740 CCACTGCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 63 GCGG CCGC(-3/-1)
226 GCGG
AfaI 131 GTAC GT^AC
882 GTAC
BciVI 345 GTATCC GTATCC(6/5)
904 GTATCC
BmyI 1254 GGGCAC G[AGT]GC[ACT]^C
1268 GAGCAC
BsiYI 531 CCAATTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1304 CCAATTCCTGG
BslI 531 CCAATTTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1304 CCAATTCCTGG
Bsp1286I 1254 GGGCAC G[AGT]GC[ACT]^C
1268 GAGCAC
BspMI 633 ACCTGC ACCTGC(4/8)
971 GCAGGT
BstYI 17 AGATCT [GA]^GATC[CT]
1040 GGATCT
MaeII 894 ACGT A^CGT
1022 ACGT
MflI 17 AGATCT [GA]^GATC[CT]
1040 GGATCT
NlaIV 881 GGTACC GG[ACGT]^[ACGT]CC
1400 GGGGCC
RsaI 131 GTAC GT^AC
882 GTAC
SfcI 41 CTACAG C^T[GA][CT]AG
168 CTGTAG
SphI 913 GCATGC GCATG^C
996 GCATGC
Tsp45I 211 GTCAC ^GT[GC]AC
260 GTGAC
XhoII 17 AGATCT [GA]^GATC[CT]
1040 GGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BfaI BsaJI BseRI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NspI PleI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I