Restriction map for
pFN21AE5247
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 97 AAATTT [GA]^AATT[CT]
AlwI 315 GATCC GGATC(4/5)
AocI 340 CCTGAGG CC^T[ACGT]AGG
ApoI 97 AAATTT [GA]^AATT[CT]
Asp700I 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 266 GAGCCC G[GA]GC[CT]^C
BclI 368 TGATCA T^GATCA
BlpI 456 GCTGAGC GC^T[ACGT]AGC
BmyI 266 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
BseRI 502 GAGGAG GAGGAG(10/8)
BsgI 196 GTGCAG GTGCAG(16/14)
BsiYI 53 CCACCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 53 CCACCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 266 GAGCCC G[AGT]GC[ACT]^C
BspMI 569 ACCTGC ACCTGC(4/8)
BsrDI 222 CATTGC GCAATG(2/0)
BsrI 515 CCAGT ACTGG(1/-1)
Bsu36I 340 CCTGAGG CC^T[ACGT]AGG
CelII 456 GCTGAGC GC^T[ACGT]AGC
Cfr13I 546 GGCCC G^G[ACGT]CC
DraII 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 54 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 91 GAAGAG CTCTTC(1/4)
Eco81I 340 CCTGAGG CC^T[ACGT]AGG
EcoO109I 545 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 368 TGATCA T^GATCA
HaeIII 546 GGCC GG^CC
HindIII 358 AAGCTT A^AGCTT
HphI 307 GGTGA GGTGA(8/7)
Ksp632I 91 GAAGAG CTCTTC(1/4)
MaeII 497 ACGT A^CGT
MspA1I 74 CAGCTG C[AC]G^C[GT]G
MwoI 355 GCCAAGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 62 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 53 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 511 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 74 CAGCTG CAG^CTG
Sau96I 546 GGCCC G^G[ACGT]CC
SfaNI 352 GATGC GCATC(5/9)
TaqI 500 TCGA T^CGA
TfiI 560 GAATC G^A[AT]TC
Tsp509I 98 AATT ^AATT
TspRI 578 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 500 TCGA T^CGA
Van91I 53 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 651 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BcnI 56 CCCGG CC^[GC]GG
462 CCGGG
BstF5I 185 GGATG GGATG(2/0)
624 GGATG
BstNI 66 CCTGG CC^[AT]GG
598 CCTGG
Cac8I 226 GCCAGC GC[ACGT]^[ACGT]GC
360 GCTTGC
DdeI 341 CTGAG C^T[ACGT]AG
457 CTGAG
DpnII 315 GATC ^GATC
369 GATC
DsaI 426 CCATGG C^C[GA][CT]GG
620 CCATGG
EcoRII 66 CCTGG ^CC[AT]GG
598 CCTGG
FokI 185 GGATG GGATG(9/13)
624 GGATG
HapII 57 CCGG C^CGG
462 CCGG
HpaII 57 CCGG C^CGG
462 CCGG
MboI 315 GATC ^GATC
369 GATC
MseI 130 TTAA T^TAA
418 TTAA
MspI 57 CCGG C^CGG
462 CCGG
MvaI 66 CCTGG CC^[AT]GG
598 CCTGG
NciI 56 CCCGG CC^[GC]GG
462 CCGGG
NcoI 426 CCATGG C^CATGG
620 CCATGG
NdeII 315 GATC ^GATC
369 GATC
NlaIII 427 CATG CATG^
621 CATG
PleI 511 GACTC GAGTC(4/5)
49 GAGTC
PstI 71 CTGCAG CTGCA^G
110 CTGCAG
Sau3AI 315 GATC ^GATC
369 GATC
Tru9I 130 TTAA T^TAA
418 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BssKI
EcoT14I Fnu4HI HinfI ItaI MboII
MnlI ScrFI SfcI StyI TseI