Restriction map for
pFN21AE1647
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HincII HindII
HinfI HpaI KasI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MluNI MscI MseI MspA1I
MunI NaeI NarI NciI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 275 GGTACC G^GTACC
AccII 199 CGCG CG^CG
AccIII 389 TCCGGA T^CCGGA
AcsI 354 GAATTC [GA]^AATT[CT]
ApoI 354 GAATTC [GA]^AATT[CT]
Asp718I 275 GGTACC G^GTACC
AvaII 264 GGACC G^G[AT]CC
BanI 275 GGTACC G^G[CT][GA]CC
BsaAI 212 CACGTA [CT]AC^GT[GA]
BsaI 376 GAGACC GGTCTC(1/5)
BsaWI 389 TCCGGA [AT]^CCGG[AT]
BseAI 389 TCCGGA T^CCGGA
BsmAI 376 GAGAC GTCTC(1/5)
BsmFI 263 GGGAC GGGAC(10/14)
Bsp1407I 291 TGTACA T^GTACA
BspEI 389 TCCGGA T^CCGGA
BspMI 457 ACCTGC ACCTGC(4/8)
BsrBI 228 CCGCTC CCGCTC(-3/-3)
BsrGI 291 TGTACA T^GTACA
BstUI 199 CGCG CG^CG
CfoI 198 GCGC GCG^C
DraII 263 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 478 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 29 CCATGG C^C[GA][CT]GG
EcoNI 145 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 263 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 354 GAATTC G^AATTC
FauI 141 CCCGC CCCGC(4/6)
HapII 390 CCGG C^CGG
HgaI 461 GCGTC GACGC(5/10)
HhaI 198 GCGC GCG^C
HinP1I 198 GCGC G^CGC
HindIII 166 AAGCTT A^AGCTT
HpaII 390 CCGG C^CGG
KpnI 275 GGTACC GGTAC^C
MaeII 213 ACGT A^CGT
MroI 389 TCCGGA T^CCGGA
MslI 345 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 390 CCGG C^CGG
MvnI 199 CGCG CG^CG
MwoI 452 GCTGCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 29 CCATGG C^CATGG
PpuMI 263 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 378 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 38 AGTACT AGT^ACT
SmlI 329 CTCAAG C^T[CT][GA]AG
SspBI 291 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 87 AGCT AG^CT
167 AGCT
BbsI 9 GAAGAC GAAGAC(2/6)
340 GAAGAC
BpuAI 9 GAAGAC GAAGAC(2/6)
340 GAAGAC
BsgI 453 CTGCAC GTGCAG(16/14)
484 GTGCAG
BsrI 300 CCAGT ACTGG(1/-1)
423 CCAGT
BstF5I 366 CATCC GGATG(2/0)
474 CATCC
Cac8I 63 GCTTGC GC[ACGT]^[ACGT]GC
140 GCCCGC
DdeI 73 CTGAG C^T[ACGT]AG
131 CTGAG
EcoT14I 29 CCATGG C^C[AT][AT]GG
402 CCAAGG
FokI 366 CATCC GGATG(9/13)
474 CATCC
HaeIII 22 GGCC GG^CC
297 GGCC
HphI 441 GGTGA GGTGA(8/7)
464 TCACC
MboII 9 GAAGA GAAGA(8/7)
340 GAAGA
SfaNI 179 GCATC GCATC(5/9)
320 GCATC
StyI 29 CCATGG C^C[AT][AT]GG
402 CCAAGG
TaqI 52 TCGA T^CGA
221 TCGA
Tsp509I 247 AATT ^AATT
355 AATT
TthHB8I 52 TCGA T^CGA
221 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AfaI BbvI BsaJI BsiYI
BslI BssKI BstNI Cfr13I EcoRII
Fnu4HI ItaI MaeIII MnlI MvaI
NlaIII NlaIV RsaI Sau96I ScrFI
TseI Tsp45I TspRI