Restriction map for
pFN21AE1640
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BmyI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 102 AAATTC [GA]^AATT[CT]
ApoI 102 AAATTC [GA]^AATT[CT]
AviII 365 TGCGCA TGC^GCA
BbsI 12 GAAGAC GAAGAC(2/6)
BcnI 125 CCCGG CC^[GC]GG
BglI 284 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 42 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTGAGC GC^T[ACGT]AGC
BpuAI 12 GAAGAC GAAGAC(2/6)
BsaWI 158 ACCGGA [AT]^CCGG[AT]
BsgI 429 CTGCAC GTGCAG(16/14)
BsmFI 337 GTCCC GGGAC(10/14)
BsrDI 233 GCAATG GCAATG(2/0)
BsrI 414 ACTGG ACTGG(1/-1)
BstYI 353 AGATCC [GA]^GATC[CT]
CelII 42 GCTGAGC GC^T[ACGT]AGC
CfoI 366 GCGC GCG^C
DraIII 454 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 422 CCGTGG C^C[GA][CT]GG
EcoRV 251 GATATC GAT^ATC
EcoT14I 217 CCTTGG C^C[AT][AT]GG
FspI 365 TGCGCA TGC^GCA
HhaI 366 GCGC GCG^C
HinP1I 366 GCGC G^CGC
HincII 139 GTCAAC GT[CT]^[GA]AC
HindII 139 GTCAAC GT[CT]^[GA]AC
MaeII 314 ACGT A^CGT
MflI 353 AGATCC [GA]^GATC[CT]
MslI 229 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 125 CCCGG CC^[GC]GG
PflMI 453 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 3 GACTC GAGTC(4/5)
StyI 217 CCTTGG C^C[AT][AT]GG
Tsp509I 103 AATT ^AATT
Van91I 453 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 353 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 231 CCGC CCGC(-3/-1)
282 CCGC
AlwI 206 GATCC GGATC(4/5)
354 GATCC
BpmI 49 CTGGAG CTGGAG(16/14)
403 CTGGAG
DraII 184 AGGTCCT [GA]G^G[ACGT]CC[CT]
239 AGGACCC
EcoO109I 184 AGGTCCT [GA]G^G[ACGT]CC[CT]
239 AGGACCC
HaeIII 54 GGCC GG^CC
462 GGCC
HphI 214 TCACC GGTGA(8/7)
440 TCACC
MaeIII 27 GTCAC ^GT[ACGT]AC
439 GTCAC
MboII 12 GAAGA GAAGA(8/7)
408 GAAGA
NlaIII 327 CATG CATG^
457 CATG
NlaIV 240 GGACCC GG[ACGT]^[ACGT]CC
372 GGAGCC
PpuMI 184 AGGTCCT [GA]G^G[AT]CC[CT]
239 AGGACCC
SfaNI 95 GATGC GCATC(5/9)
296 GCATC
TfiI 71 GAATC G^A[AT]TC
222 GAATC
Tsp45I 27 GTCAC ^GT[GC]AC
439 GTCAC
TspRI 29 CACTG CA[GC]TG[ACGT][ACGT]^
382 CACTG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AvaII BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HapII
HinfI HpaII ItaI MboI MnlI
MspI MvaI MwoI NdeII Sau3AI
Sau96I ScrFI TseI