Restriction map for
pFN21AB5806
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BbrPI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SalI SbfI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 1039 GAATTC [GA]^AATT[CT]
AlwNI 1316 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 493 GGGCCC GGGCC^C
ApoI 1039 GAATTC [GA]^AATT[CT]
AvaI 902 CTCGGG C^[CT]CG[GA]G
BamHI 1078 GGATCC G^GATCC
BbeI 707 GGCGCC GGCGC^C
BbsI 836 GAAGAC GAAGAC(2/6)
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BcgI 894 GCACTTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1142 TGATCA T^GATCA
BpuAI 836 GAAGAC GAAGAC(2/6)
BsiEI 248 CGGCCG CG[GA][CT]^CG
BsmAI 1119 GAGAC GTCTC(1/5)
BsmI 464 GAATGC GAATGC(1/-1)
BsoBI 902 CTCGGG C^[CT]CG[GA]G
BspHI 440 TCATGA T^CATGA
BspMI 344 ACCTGC ACCTGC(4/8)
BsrDI 690 CATTGC GCAATG(2/0)
BsrFI 151 ACCGGC [GA]^CCGG[CT]
BstAPI 1168 GCAATATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 684 GGTGACC G^GT[ACGT]ACC
BstF5I 1113 CATCC GGATG(2/0)
BstPI 684 GGTGACC G^GT[ACGT]ACC
Cfr10I 151 ACCGGC [GA]^CCGG[CT]
DraII 492 AGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 248 CGGCCG C^GGCCG
EclXI 248 CGGCCG C^GGCCG
Eco52I 248 CGGCCG C^GGCCG
EcoO109I 492 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 684 GGTGACC G^GT[ACGT]ACC
EcoRI 1039 GAATTC G^AATTC
FbaI 1142 TGATCA T^GATCA
FokI 1113 CATCC GGATG(9/13)
KasI 707 GGCGCC G^GCGCC
KspI 515 CCGCGG CCGC^GG
MaeII 42 ACGT A^CGT
NarI 707 GGCGCC GG^CGCC
NcoI 447 CCATGG C^CATGG
NdeI 973 CATATG CA^TATG
NspI 1278 ACATGC [GA]CATG^[CT]
RcaI 440 TCATGA T^CATGA
SacII 515 CCGCGG CCGC^GG
SapI 761 GAAGAGC GCTCTTC(1/4)
ScaI 299 AGTACT AGT^ACT
SfoI 707 GGCGCC GGC^GCC
SpeI 21 ACTAGT A^CTAGT
TaqI 410 TCGA T^CGA
TfiI 1264 GATTC G^A[AT]TC
TthHB8I 410 TCGA T^CGA
XbaI 1101 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 356 CGCG CG^CG
516 CGCG
AcyI 262 GACGCC G[GA]^CG[CT]C
707 GGCGCC
AfaI 195 GTAC GT^AC
300 GTAC
BalI 573 TGGCCA TGG^CCA
1269 TGGCCA
BcnI 16 CCGGG CC^[GC]GG
246 CCCGG
BfaI 22 CTAG C^TAG
1102 CTAG
BpmI 1083 CTCCAG CTGGAG(16/14)
88 CTGGAG
BsaHI 262 GACGCC G[GA]^CG[CT]C
707 GGCGCC
BseRI 123 CTCCTC GAGGAG(10/8)
126 CTCCTC
BsgI 346 CTGCAC GTGCAG(16/14)
1175 GTGCAG
BsrBI 202 CCGCTC CCGCTC(-3/-3)
1256 GAGCGG
BstUI 356 CGCG CG^CG
516 CGCG
BstYI 311 AGATCC [GA]^GATC[CT]
1078 GGATCC
Cac8I 357 GCGGGC GC[ACGT]^[ACGT]GC
507 GCTGGC
EarI 593 GAAGAG CTCTTC(1/4)
761 GAAGAG
HaeII 360 GGCGCT [GA]GCGC^[CT]
707 GGCGCC
HgaI 262 GACGC GACGC(5/10)
166 GCGTC
Hin1I 262 GACGCC G[GA]^CG[CT]C
707 GGCGCC
HincII 3 GTCAAC GT[CT]^[GA]AC
613 GTCAAC
HindII 3 GTCAAC GT[CT]^[GA]AC
613 GTCAAC
Ksp632I 593 GAAGAG CTCTTC(1/4)
761 GAAGAG
MaeI 22 CTAG C^TAG
1102 CTAG
MflI 311 AGATCC [GA]^GATC[CT]
1078 GGATCC
MluNI 573 TGGCCA TGG^CCA
1269 TGGCCA
MscI 573 TGGCCA TGG^CCA
1269 TGGCCA
MslI 603 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
719 CACCTGAGTG
MspA1I 515 CCGCGG C[AC]G^C[GT]G
786 CCGCTG
MvnI 356 CGCG CG^CG
516 CGCG
NciI 16 CCGGG CC^[GC]GG
246 CCCGG
RsaI 195 GTAC GT^AC
300 GTAC
SmlI 364 CTCAAG C^T[CT][GA]AG
1053 CTTGAG
XcmI 497 CCAGCCTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
965 CCAGACAACATATGG
XhoII 311 AGATCC [GA]^GATC[CT]
1078 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI BanI BanII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I Tsp509I TspRI