Restriction map for
pFN21AE9456
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbvCI BcgI
BciVI BfrI BglII BlnI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CfoI
Cfr10I CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XcmI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 654 GAATTT [GA]^AATT[CT]
AocI 1636 CCTGAGG CC^T[ACGT]AGG
ApoI 654 GAATTT [GA]^AATT[CT]
AseI 2067 ATTAAT AT^TAAT
AsnI 2067 ATTAAT AT^TAAT
AspHI 368 GAGCTC G[AT]GC[AT]^C
BamHI 140 GGATCC G^GATCC
BanI 1571 GGTGCC G^G[CT][GA]CC
BbrPI 1318 CACGTG CAC^GTG
BglI 1687 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1224 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1224 GCTCAGC GC^T[ACGT]AGC
BsaAI 1318 CACGTG [CT]AC^GT[GA]
BsiHKAI 368 GAGCTC G[AT]GC[AT]^C
BsmBI 1230 CGTCTC CGTCTC(1/5)
BsmI 2034 GAATGC GAATGC(1/-1)
Bsp1407I 268 TGTACA T^GTACA
BspDI 1182 ATCGAT AT^CGAT
BspMI 1213 ACCTGC ACCTGC(4/8)
BsrGI 268 TGTACA T^GTACA
BstBI 1006 TTCGAA TT^CGAA
BstXI 1041 CCAGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1636 CCTGAGG CC^T[ACGT]AGG
CelII 1224 GCTCAGC GC^T[ACGT]AGC
ClaI 1182 ATCGAT AT^CGAT
DraIII 1272 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1636 CCTGAGG CC^T[ACGT]AGG
EcoT14I 890 CCTTGG C^C[AT][AT]GG
FauI 1025 CCCGC CCCGC(4/6)
HincII 1744 GTCAAC GT[CT]^[GA]AC
HindII 1744 GTCAAC GT[CT]^[GA]AC
HindIII 1242 AAGCTT A^AGCTT
NspV 1006 TTCGAA TT^CGAA
PaeR7I 536 CTCGAG C^TCGAG
PmaCI 1318 CACGTG CAC^GTG
PmlI 1318 CACGTG CAC^GTG
PpuMI 159 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2067 ATTAAT AT^TAAT
PstI 183 CTGCAG CTGCA^G
SacI 368 GAGCTC GAGCT^C
SfuI 1006 TTCGAA TT^CGAA
SmaI 1283 CCCGGG CCC^GGG
SspBI 268 TGTACA T^GTACA
StuI 1640 AGGCCT AGG^CCT
StyI 890 CCTTGG C^C[AT][AT]GG
XbaI 357 TCTAGA T^CTAGA
XhoI 536 CTCGAG C^TCGAG
XmaCI 1283 CCCGGG C^CCGGG
XmaI 1283 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 1026 CCGC CCGC(-3/-1)
1128 CCGC
AfaI 269 GTAC GT^AC
817 GTAC
AvaI 536 CTCGAG C^[CT]CG[GA]G
1283 CCCGGG
AvaII 160 GGACC G^G[AT]CC
1280 GGTCC
BalI 566 TGGCCA TGG^CCA
850 TGGCCA
BbsI 932 GTCTTC GAAGAC(2/6)
2001 GTCTTC
BclI 1259 TGATCA T^GATCA
1580 TGATCA
BfaI 358 CTAG C^TAG
2044 CTAG
BpmI 371 CTCCAG CTGGAG(16/14)
95 CTGGAG
BpuAI 932 GTCTTC GAAGAC(2/6)
2001 GTCTTC
BsaI 376 GAGACC GGTCTC(1/5)
1632 GAGACC
BsoBI 536 CTCGAG C^[CT]CG[GA]G
1283 CCCGGG
DraII 159 AGGACCT [GA]G^G[ACGT]CC[CT]
1756 AGGCCCT
EaeI 566 TGGCCA [CT]^GGCC[GA]
850 TGGCCA
EcoO109I 159 AGGACCT [GA]G^G[ACGT]CC[CT]
1756 AGGCCCT
EcoT22I 857 ATGCAT ATGCA^T
2036 ATGCAT
FbaI 1259 TGATCA T^GATCA
1580 TGATCA
HapII 1284 CCGG C^CGG
1693 CCGG
HgaI 1229 GCGTC GACGC(5/10)
1314 GCGTC
HpaII 1284 CCGG C^CGG
1693 CCGG
MaeI 358 CTAG C^TAG
2044 CTAG
MaeII 1319 ACGT A^CGT
1894 ACGT
MluNI 566 TGGCCA TGG^CCA
850 TGGCCA
MscI 566 TGGCCA TGG^CCA
850 TGGCCA
MspI 1284 CCGG C^CGG
1693 CCGG
NsiI 857 ATGCAT ATGCA^T
2036 ATGCAT
PleI 301 GACTC GAGTC(4/5)
930 GAGTC
PvuII 257 CAGCTG CAG^CTG
2027 CAGCTG
RsaI 269 GTAC GT^AC
817 GTAC
SapI 549 GAAGAGC GCTCTTC(1/4)
1473 GCTCTTC
SmlI 395 CTCAAG C^T[CT][GA]AG
536 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AflIII AluI AlwI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoRV Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII