Restriction map for
pFN21AB5744
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AocI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsiWI
BspDI BspHI BsrBI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI DraI DrdI EagI EclXI
Eco52I Eco81I EcoRI EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
KasI KpnI MamI MluI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 969 CGCG CG^CG
AflIII 405 ACATGT A^C[GA][CT]GT
Alw44I 2140 GTGCAC G^TGCAC
AlwNI 1024 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2120 AGCGCT AGC^GCT
ApaI 866 GGGCCC GGGCC^C
ApaLI 2140 GTGCAC G^TGCAC
Asp700I 1055 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 960 CTCGGG C^[CT]CG[GA]G
BbvCI 1794 CCTCAGC CCTCAGC(-5/-2)
BcgI 2159 GCATGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 513 CCGGG CC^[GC]GG
BfaI 1261 CTAG C^TAG
BseRI 1734 GAGGAG GAGGAG(10/8)
BsiEI 1957 CGGTCG CG[GA][CT]^CG
BsmBI 307 CGTCTC CGTCTC(1/5)
BsoBI 960 CTCGGG C^[CT]CG[GA]G
BspLU11I 405 ACATGT A^CATGT
BsrDI 1106 GCAATG GCAATG(2/0)
BstAPI 1023 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 969 CGCG CG^CG
CfoI 2121 GCGC GCG^C
CpoI 509 CGGACCG CG^G[AT]CCG
DraII 1974 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 216 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 968 CCGCGG C^C[GA][CT]GG
EarI 973 GAAGAG CTCTTC(1/4)
Eco47III 2120 AGCGCT AGC^GCT
EcoO109I 1974 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1783 GATATC GAT^ATC
HaeII 2120 AGCGCT [GA]GCGC^[CT]
HhaI 2121 GCGC GCG^C
HinP1I 2121 GCGC G^CGC
HincII 1404 GTTAAC GT[CT]^[GA]AC
HindII 1404 GTTAAC GT[CT]^[GA]AC
HindIII 1357 AAGCTT A^AGCTT
HpaI 1404 GTTAAC GTT^AAC
Ksp632I 973 GAAGAG CTCTTC(1/4)
KspI 968 CCGCGG CCGC^GG
MaeI 1261 CTAG C^TAG
MvnI 969 CGCG CG^CG
NaeI 931 GCCGGC GCC^GGC
NciI 513 CCGGG CC^[GC]GG
NgoMIV 931 GCCGGC G^CCGGC
PflFI 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 402 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 59 CAGCTG CAG^CTG
RsrII 509 CGGACCG CG^G[AT]CCG
SacI 348 GAGCTC GAGCT^C
SacII 968 CCGCGG CCGC^GG
ScaI 2003 AGTACT AGT^ACT
SexAI 217 ACCAGGT A^CC[AT]GGT
SphI 474 GCATGC GCATG^C
StuI 1842 AGGCCT AGG^CCT
Tth111I 1618 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 402 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 211 CCAAACACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1055 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 1313 TCCGGA T^CCGGA
1550 TCCGGA
BalI 429 TGGCCA TGG^CCA
1996 TGGCCA
BanI 844 GGCACC G^G[CT][GA]CC
928 GGTGCC
BglI 442 GCCTATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1835 GCCCCCAAGGC
BsaWI 1313 TCCGGA [AT]^CCGG[AT]
1550 TCCGGA
BseAI 1313 TCCGGA T^CCGGA
1550 TCCGGA
Bsp1407I 93 TGTACA T^GTACA
767 TGTACA
BspEI 1313 TCCGGA T^CCGGA
1550 TCCGGA
BsrFI 931 GCCGGC [GA]^CCGG[CT]
1497 GCCGGT
BsrGI 93 TGTACA T^GTACA
767 TGTACA
BssSI 463 CACGAG CACGAG(-5/-1)
493 CACGAG
BstEII 397 GGTGACC G^GT[ACGT]ACC
556 GGTTACC
BstPI 397 GGTGACC G^GT[ACGT]ACC
556 GGTTACC
Cfr10I 931 GCCGGC [GA]^CCGG[CT]
1497 GCCGGT
EcoO65I 397 GGTGACC G^GT[ACGT]ACC
556 GGTTACC
MaeII 226 ACGT A^CGT
2060 ACGT
MfeI 771 CAATTG C^AATTG
1158 CAATTG
MluNI 429 TGGCCA TGG^CCA
1996 TGGCCA
MroI 1313 TCCGGA T^CCGGA
1550 TCCGGA
MscI 429 TGGCCA TGG^CCA
1996 TGGCCA
MspA1I 59 CAGCTG C[AC]G^C[GT]G
968 CCGCGG
MunI 771 CAATTG C^AATTG
1158 CAATTG
PstI 146 CTGCAG CTGCA^G
2079 CTGCAG
SfcI 146 CTGCAG C^T[GA][CT]AG
2079 CTGCAG
SspBI 93 TGTACA T^GTACA
767 TGTACA
Tsp45I 398 GTGAC ^GT[GC]AC
2021 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI ApoI AspEI AspHI AvaII
BanII BbvI BmyI BpmI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
Eam1105I EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII