Restriction map for
pFN21AE5446
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 892 AAATTT [GA]^AATT[CT]
AfaI 496 GTAC GT^AC
ApoI 892 AAATTT [GA]^AATT[CT]
Asp700I 803 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 774 GAGCTC G[AT]GC[AT]^C
AvaI 880 CCCGGG C^[CT]CG[GA]G
BalI 660 TGGCCA TGG^CCA
BamHI 757 GGATCC G^GATCC
BfaI 103 CTAG C^TAG
BglI 445 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 774 GAGCTC G[AT]GC[AT]^C
BsoBI 880 CCCGGG C^[CT]CG[GA]G
BstAPI 201 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 883 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 793 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 883 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 805 ATGCAT ATGCA^T
FauI 399 CCCGC CCCGC(4/6)
HaeII 485 GGCGCT [GA]GCGC^[CT]
HgaI 537 GACGC GACGC(5/10)
HphI 231 GGTGA GGTGA(8/7)
MaeI 103 CTAG C^TAG
MaeIII 316 GTCAC ^GT[ACGT]AC
MluNI 660 TGGCCA TGG^CCA
MscI 660 TGGCCA TGG^CCA
NsiI 805 ATGCAT ATGCA^T
PflMI 269 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 537 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 18 CTGCAG CTGCA^G
RsaI 496 GTAC GT^AC
SacI 774 GAGCTC GAGCT^C
SfaNI 395 GCATC GCATC(5/9)
SfcI 18 CTGCAG C^T[GA][CT]AG
SmaI 880 CCCGGG CCC^GGG
SmlI 704 CTCAAG C^T[CT][GA]AG
Tsp45I 316 GTCAC ^GT[GC]AC
Tsp509I 893 AATT ^AATT
Van91I 269 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 717 CCAAACACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 880 CCCGGG C^CCGGG
XmaI 880 CCCGGG C^CCGGG
XmnI 803 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanI 82 GGTGCC G^G[CT][GA]CC
342 GGCACC
BsgI 956 CTGCAC GTGCAG(16/14)
208 GTGCAG
BsmI 803 GAATGC GAATGC(1/-1)
807 GCATTC
BstYI 749 AGATCC [GA]^GATC[CT]
757 GGATCC
CfoI 156 GCGC GCG^C
486 GCGC
HapII 36 CCGG C^CGG
881 CCGG
HhaI 156 GCGC GCG^C
486 GCGC
HinP1I 156 GCGC G^CGC
486 GCGC
HpaII 36 CCGG C^CGG
881 CCGG
MaeII 407 ACGT A^CGT
785 ACGT
MflI 749 AGATCC [GA]^GATC[CT]
757 GGATCC
MseI 404 TTAA T^TAA
671 TTAA
MspA1I 21 CAGCTG C[AC]G^C[GT]G
97 CAGCTG
MspI 36 CCGG C^CGG
881 CCGG
PvuII 21 CAGCTG CAG^CTG
97 CAGCTG
Tru9I 404 TTAA T^TAA
671 TTAA
TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^
187 CAGTG
XhoII 749 AGATCC [GA]^GATC[CT]
757 GGATCC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII ItaI MboI
MboII MnlI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI TseI