Restriction map for
pFN21AE4122
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HgaI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 358 GACGTC GACGT^C
AcyI 358 GACGTC G[GA]^CG[CT]C
AocI 432 CCTGAGG CC^T[ACGT]AGG
Aor51HI 37 AGCGCT AGC^GCT
ApaI 521 GGGCCC GGGCC^C
AseI 931 ATTAAT AT^TAAT
AsnI 931 ATTAAT AT^TAAT
Asp700I 874 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 74 GAGCAC G[AT]GC[AT]^C
AspI 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 338 GGTCC G^G[AT]CC
BalI 622 TGGCCA TGG^CCA
BanII 521 GGGCCC G[GA]GC[CT]^C
BbsI 961 GTCTTC GAAGAC(2/6)
BciVI 786 GGATAC GTATCC(6/5)
BclI 1346 TGATCA T^GATCA
BlpI 40 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 40 GCTAAGC GC^T[ACGT]AGC
BpuAI 961 GTCTTC GAAGAC(2/6)
BsaAI 1327 TACGTG [CT]AC^GT[GA]
BsaHI 358 GACGTC G[GA]^CG[CT]C
BsaI 1390 GGTCTC GGTCTC(1/5)
BsiHKAI 74 GAGCAC G[AT]GC[AT]^C
BsmI 56 GCATTC GAATGC(1/-1)
Bsp1407I 858 TGTACA T^GTACA
BspHI 177 TCATGA T^CATGA
BspLU11I 1481 ACATGT A^CATGT
BsrGI 858 TGTACA T^GTACA
Bsu36I 432 CCTGAGG CC^T[ACGT]AGG
CelII 40 GCTAAGC GC^T[ACGT]AGC
CfoI 38 GCGC GCG^C
DrdI 1008 GACCAACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 97 CCACGG C^C[GA][CT]GG
Eco47III 37 AGCGCT AGC^GCT
Eco81I 432 CCTGAGG CC^T[ACGT]AGG
EcoRI 878 GAATTC G^AATTC
EcoT14I 817 CCAAGG C^C[AT][AT]GG
FbaI 1346 TGATCA T^GATCA
HaeII 37 AGCGCT [GA]GCGC^[CT]
HhaI 38 GCGC GCG^C
Hin1I 358 GACGTC G[GA]^CG[CT]C
HinP1I 38 GCGC G^CGC
MluNI 622 TGGCCA TGG^CCA
MscI 622 TGGCCA TGG^CCA
PflFI 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 662 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 931 ATTAAT AT^TAAT
PvuII 403 CAGCTG CAG^CTG
RcaI 177 TCATGA T^CATGA
SapI 1141 GAAGAGC GCTCTTC(1/4)
SexAI 938 ACCTGGT A^CC[AT]GGT
SspBI 858 TGTACA T^GTACA
SspI 731 AATATT AAT^ATT
StuI 164 AGGCCT AGG^CCT
StyI 817 CCAAGG C^C[AT][AT]GG
Tth111I 937 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 300 TCTAGA T^CTAGA
XcmI 903 CCACCCTGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 874 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 1328 ACGTGT A^C[GA][CT]GT
1481 ACATGT
AlwI 497 GGATC GGATC(4/5)
504 GGATC
BglII 866 AGATCT A^GATCT
1364 AGATCT
BmyI 74 GAGCAC G[AGT]GC[ACT]^C
521 GGGCCC
BsgI 1263 CTGCAC GTGCAG(16/14)
1334 GTGCAG
Bsp1286I 74 GAGCAC G[AGT]GC[ACT]^C
521 GGGCCC
DraII 520 GGGGCCC [GA]G^G[ACGT]CC[CT]
521 GGGCCCC
EaeI 2 TGGCCG [CT]^GGCC[GA]
622 TGGCCA
EcoO109I 520 GGGGCCC [GA]G^G[ACGT]CC[CT]
521 GGGCCCC
MaeIII 943 GTCAC ^GT[ACGT]AC
1006 GTGAC
MseI 119 TTAA T^TAA
932 TTAA
SmlI 1019 CTCAAG C^T[CT][GA]AG
1474 CTCAAG
TaqI 645 TCGA T^CGA
765 TCGA
Tru9I 119 TTAA T^TAA
932 TTAA
Tsp45I 943 GTCAC ^GT[GC]AC
1006 GTGAC
TthHB8I 645 TCGA T^CGA
765 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BcnI BfaI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp509I
TspRI XhoII